Human Gene GPR85 (ENST00000424100.2_7) from GENCODE V47lift37
  Description: G protein-coupled receptor 85, transcript variant 4 (from RefSeq NM_001146267.2)
Gencode Transcript: ENST00000424100.2_7
Gencode Gene: ENSG00000164604.14_16
Transcript (Including UTRs)
   Position: hg19 chr7:112,722,340-112,726,882 Size: 4,543 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr7:112,723,664-112,724,776 Size: 1,113 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:112,722,340-112,726,882)mRNA (may differ from genome)Protein (370 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCHuman Cortex Gene ExpressionMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPR85_HUMAN
DESCRIPTION: RecName: Full=Probable G-protein coupled receptor 85; AltName: Full=Super conserved receptor expressed in brain 2;
FUNCTION: Orphan receptor.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in brain and testis. Lower levels in small intestine, placenta and spleen. In brain regions, detected in all regions tested, but somewhat lower levels in the corpus callosum, medulla and spinal cord.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.29 RPKM in Brain - Caudate (basal ganglia)
Total median expression: 42.20 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -262.60794-0.331 Picture PostScript Text
3' UTR -305.501324-0.231 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P60893
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005515 protein binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC030577 - Homo sapiens G protein-coupled receptor 85, mRNA (cDNA clone MGC:26047 IMAGE:4823671), complete cds.
AF250237 - Homo sapiens orphan G protein-coupled receptor 85 (GPR85) mRNA, complete cds.
AL161959 - Homo sapiens mRNA; cDNA DKFZp761L08121 (from clone DKFZp761L08121).
JD552722 - Sequence 533746 from Patent EP1572962.
JD229377 - Sequence 210401 from Patent EP1572962.
JD435715 - Sequence 416739 from Patent EP1572962.
JD403478 - Sequence 384502 from Patent EP1572962.
AK297464 - Homo sapiens cDNA FLJ50993 complete cds, moderately similar to Probable G-protein coupled receptor 85.
AK302769 - Homo sapiens cDNA FLJ51159 complete cds, moderately similar to Probable G-protein coupled receptor 85.
JD045339 - Sequence 26363 from Patent EP1572962.
AK309392 - Homo sapiens cDNA, FLJ99433.
AB040800 - Homo sapiens mRNA for SREB2, complete cds.
AK312587 - Homo sapiens cDNA, FLJ92962, Homo sapiens G protein-coupled receptor 85 (GPR85), mRNA.
KJ898973 - Synthetic construct Homo sapiens clone ccsbBroadEn_08367 GPR85 gene, encodes complete protein.
AB384980 - Synthetic construct DNA, clone: pF1KB4699, Homo sapiens GPR85 gene for G-protein coupled receptor 85, complete cds, without stop codon, in Flexi system.
AM393528 - Synthetic construct Homo sapiens clone IMAGE:100001880 for hypothetical protein (GPR85 gene).
AM393553 - Synthetic construct Homo sapiens clone IMAGE:100001886 for hypothetical protein (GPR85 gene).
CU688912 - Synthetic construct Homo sapiens gateway clone IMAGE:100019110 5' read GPR85 mRNA.
JD533416 - Sequence 514440 from Patent EP1572962.
JD175055 - Sequence 156079 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000424100.1, GPR85_HUMAN, NM_001146267, P60893, Q9JHI6, Q9NPD1, SREB2, uc319tjg.1, uc319tjg.2
UCSC ID: ENST00000424100.2_7
RefSeq Accession: NM_001146267.2
Protein: P60893 (aka GPR85_HUMAN or GP85_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.