Human Gene ADGRA2 (ENST00000315215.11_1) from GENCODE V47lift37
  Description: Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628). (from UniProt Q96PE1)
Gencode Transcript: ENST00000315215.11_1
Gencode Gene: ENSG00000020181.18_8
Transcript (Including UTRs)
   Position: hg19 chr8:37,654,424-37,702,414 Size: 47,991 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg19 chr8:37,654,787-37,699,873 Size: 45,087 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:37,654,424-37,702,414)mRNA (may differ from genome)Protein (1121 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: GP124_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled receptor 124; AltName: Full=Tumor endothelial marker 5; Flags: Precursor;
FUNCTION: Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity).
SUBUNIT: Interacts with DLG1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon.
DOMAIN: Binds to DLG1 through its PDZ-binding motif.
SIMILARITY: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
SIMILARITY: Contains 1 GPS domain.
SIMILARITY: Contains 1 Ig-like (immunoglobulin-like) domain.
SIMILARITY: Contains 4 LRR (leucine-rich) repeats.
SIMILARITY: Contains 1 LRRCT domain.
SIMILARITY: Contains 1 LRRNT domain.
CAUTION: It is uncertain whether Met-1 or Met-8 is the initiator.
SEQUENCE CAUTION: Sequence=AAI46775.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAL11992.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAO27354.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA96055.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 37.86 RPKM in Cervix - Endocervix
Total median expression: 405.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -210.70363-0.580 Picture PostScript Text
3' UTR -928.402541-0.365 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000483 - Cys-rich_flank_reg_C
IPR017981 - GPCR_2-like
IPR001879 - GPCR_2_extracellular_dom
IPR000832 - GPCR_2_secretin-like
IPR017983 - GPCR_2_secretin-like_CS
IPR000203 - GPS_dom
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR003599 - Ig_sub
IPR001611 - Leu-rich_rpt
IPR003591 - Leu-rich_rpt_typical-subtyp

Pfam Domains:
PF00002 - 7 transmembrane receptor (Secretin family)
PF00560 - Leucine Rich Repeat
PF13855 - Leucine rich repeat

SCOP Domains:
48726 - Immunoglobulin
52047 - RNI-like
52058 - L domain-like
52075 - Outer arm dynein light chain 1
111418 - Hormone receptor domain

ModBase Predicted Comparative 3D Structure on Q96PE1
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004888 transmembrane signaling receptor activity
GO:0004930 G-protein coupled receptor activity
GO:0005515 protein binding

Biological Process:
GO:0001525 angiogenesis
GO:0002040 sprouting angiogenesis
GO:0007165 signal transduction
GO:0007166 cell surface receptor signaling pathway
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007417 central nervous system development
GO:0010595 positive regulation of endothelial cell migration
GO:0043542 endothelial cell migration
GO:0045765 regulation of angiogenesis
GO:0050920 regulation of chemotaxis
GO:0090210 regulation of establishment of blood-brain barrier
GO:1900747 negative regulation of vascular endothelial growth factor signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030175 filopodium
GO:0042995 cell projection


-  Descriptions from all associated GenBank mRNAs
  AK027296 - Homo sapiens cDNA FLJ14390 fis, clone HEMBA1003071, weakly similar to INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN PRECURSOR (ALS).
MS636124 - Sequence 1 from Patent WO2017040916.
AB040964 - Homo sapiens mRNA for KIAA1531 protein, partial cds.
BC146774 - Homo sapiens G protein-coupled receptor 124, mRNA (cDNA clone MGC:166853 IMAGE:9007223), complete cds.
AY090637 - Homo sapiens tumor endothelial marker 5 precursor (TEM5) mRNA, partial cds.
AF378755 - Homo sapiens tumor endothelial marker 5 precursor (TEM5) mRNA, complete cds.
AB385479 - Synthetic construct DNA, clone: pF1KA1531, Homo sapiens GPR124 gene for G-protein coupled receptor 124, complete cds, without stop codon, in Flexi system.
AY181242 - Homo sapiens G protein-coupled receptor 124 (GPR124) mRNA, complete cds.
AK074099 - Homo sapiens mRNA for FLJ00170 protein.
KU257672 - Homo sapiens tumor endothelial marker 5 precursor (TEM5) mRNA, partial cds.
AL832959 - Homo sapiens mRNA; cDNA DKFZp666M0710 (from clone DKFZp666M0710).
AL832524 - Homo sapiens mRNA; cDNA DKFZp761L0917 (from clone DKFZp761L0917).
AL110244 - Homo sapiens mRNA; cDNA DKFZp434C211 (from clone DKFZp434C211).
JD389945 - Sequence 370969 from Patent EP1572962.
JD374650 - Sequence 355674 from Patent EP1572962.
JD544478 - Sequence 525502 from Patent EP1572962.
JD385809 - Sequence 366833 from Patent EP1572962.
JD382895 - Sequence 363919 from Patent EP1572962.
JD399622 - Sequence 380646 from Patent EP1572962.
AL137610 - Homo sapiens mRNA; cDNA DKFZp434J0911 (from clone DKFZp434J0911).
JD374155 - Sequence 355179 from Patent EP1572962.
JD498942 - Sequence 479966 from Patent EP1572962.
JD096950 - Sequence 77974 from Patent EP1572962.
JD498095 - Sequence 479119 from Patent EP1572962.
JD241462 - Sequence 222486 from Patent EP1572962.
JD224785 - Sequence 205809 from Patent EP1572962.
JD219871 - Sequence 200895 from Patent EP1572962.
JD529967 - Sequence 510991 from Patent EP1572962.
JD435620 - Sequence 416644 from Patent EP1572962.
JD049523 - Sequence 30547 from Patent EP1572962.
JD043625 - Sequence 24649 from Patent EP1572962.
JD314241 - Sequence 295265 from Patent EP1572962.
JD127642 - Sequence 108666 from Patent EP1572962.
JD444151 - Sequence 425175 from Patent EP1572962.
JD511766 - Sequence 492790 from Patent EP1572962.
JD456855 - Sequence 437879 from Patent EP1572962.
JD347078 - Sequence 328102 from Patent EP1572962.
JD276765 - Sequence 257789 from Patent EP1572962.
JD276766 - Sequence 257790 from Patent EP1572962.
JD303742 - Sequence 284766 from Patent EP1572962.
JD239415 - Sequence 220439 from Patent EP1572962.
JD381144 - Sequence 362168 from Patent EP1572962.
JD078802 - Sequence 59826 from Patent EP1572962.
JD499558 - Sequence 480582 from Patent EP1572962.
JD363604 - Sequence 344628 from Patent EP1572962.
JD428606 - Sequence 409630 from Patent EP1572962.
JD509275 - Sequence 490299 from Patent EP1572962.
CU679784 - Synthetic construct Homo sapiens gateway clone IMAGE:100017226 3' read BRF2 mRNA.

-  Other Names for This Gene
  Alternate Gene Symbols: A6H8W3, ADGRA2 , AGRA2_HUMAN, D3DSW4, ENST00000315215.1, ENST00000315215.10, ENST00000315215.2, ENST00000315215.3, ENST00000315215.4, ENST00000315215.5, ENST00000315215.6, ENST00000315215.7, ENST00000315215.8, ENST00000315215.9, GPR124 , KIAA1531 , MS636124, Q8N3R1, Q8TEM3, Q96KB2, Q96PE1, Q9P1Z7, Q9UFY4, TEM5 , uc317ptt.1
UCSC ID: ENST00000315215.11_1
RefSeq Accession: NM_032777.10
Protein: Q96PE1 (aka GP124_HUMAN or G124_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.