Human Gene GPER1 (ENST00000397088.4_6) from GENCODE V47lift37
  Description: G protein-coupled estrogen receptor 1, transcript variant 4 (from RefSeq NM_001098201.3)
Gencode Transcript: ENST00000397088.4_6
Gencode Gene: ENSG00000164850.16_11
Transcript (Including UTRs)
   Position: hg19 chr7:1,127,749-1,133,446 Size: 5,698 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr7:1,131,365-1,132,492 Size: 1,128 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:1,127,749-1,133,446)mRNA (may differ from genome)Protein (375 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPER_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled estrogen receptor 1; AltName: Full=Chemoattractant receptor-like 2; AltName: Full=Flow-induced endothelial G-protein coupled receptor 1; Short=FEG-1; AltName: Full=G-protein coupled receptor 30; AltName: Full=GPCR-BR; AltName: Full=IL8-related receptor DRY12; AltName: Full=Lymphocyte-derived G-protein coupled receptor; Short=LYGPR; AltName: Full=Membrane estrogen receptor; Short=mER;
FUNCTION: Receptor for estrogen.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi-pass membrane protein (By similarity). Note=Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
TISSUE SPECIFICITY: Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GPER1
Diseases sorted by gene-association score: breast cancer (2)

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.93 RPKM in Stomach
Total median expression: 217.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -215.10531-0.405 Picture PostScript Text
3' UTR -379.10954-0.397 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q99527
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003682 chromatin binding
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005496 steroid binding
GO:0005515 protein binding
GO:0017082 mineralocorticoid receptor activity
GO:0030284 estrogen receptor activity
GO:1990239 steroid hormone binding

Biological Process:
GO:0001934 positive regulation of protein phosphorylation
GO:0001956 positive regulation of neurotransmitter secretion
GO:0002376 immune system process
GO:0002695 negative regulation of leukocyte activation
GO:0006915 apoptotic process
GO:0006954 inflammatory response
GO:0007049 cell cycle
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0007399 nervous system development
GO:0008284 positive regulation of cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010629 negative regulation of gene expression
GO:0010948 negative regulation of cell cycle process
GO:0014068 positive regulation of phosphatidylinositol 3-kinase signaling
GO:0019228 neuronal action potential
GO:0030154 cell differentiation
GO:0030263 apoptotic chromosome condensation
GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
GO:0030335 positive regulation of cell migration
GO:0030518 intracellular steroid hormone receptor signaling pathway
GO:0030819 positive regulation of cAMP biosynthetic process
GO:0031959 mineralocorticoid receptor signaling pathway
GO:0032024 positive regulation of insulin secretion
GO:0032962 positive regulation of inositol trisphosphate biosynthetic process
GO:0043065 positive regulation of apoptotic process
GO:0043280 positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043401 steroid hormone mediated signaling pathway
GO:0043410 positive regulation of MAPK cascade
GO:0045087 innate immune response
GO:0045599 negative regulation of fat cell differentiation
GO:0045742 positive regulation of epidermal growth factor receptor signaling pathway
GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050728 negative regulation of inflammatory response
GO:0050769 positive regulation of neurogenesis
GO:0051053 negative regulation of DNA metabolic process
GO:0051055 negative regulation of lipid biosynthetic process
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
GO:0051480 regulation of cytosolic calcium ion concentration
GO:0051898 negative regulation of protein kinase B signaling
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0070474 positive regulation of uterine smooth muscle contraction
GO:0071157 negative regulation of cell cycle arrest
GO:0071333 cellular response to glucose stimulus
GO:0071356 cellular response to tumor necrosis factor
GO:0071375 cellular response to peptide hormone stimulus
GO:0071389 cellular response to mineralocorticoid stimulus
GO:0071392 cellular response to estradiol stimulus
GO:0090200 positive regulation of release of cytochrome c from mitochondria
GO:1903078 positive regulation of protein localization to plasma membrane
GO:1904706 negative regulation of vascular smooth muscle cell proliferation
GO:2000353 positive regulation of endothelial cell apoptotic process
GO:2000724 positive regulation of cardiac vascular smooth muscle cell differentiation
GO:2001238 positive regulation of extrinsic apoptotic signaling pathway

Cellular Component:
GO:0000139 Golgi membrane
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005768 endosome
GO:0005769 early endosome
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0005802 trans-Golgi network
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0014069 postsynaptic density
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030054 cell junction
GO:0030424 axon
GO:0030425 dendrite
GO:0030659 cytoplasmic vesicle membrane
GO:0031410 cytoplasmic vesicle
GO:0031966 mitochondrial membrane
GO:0032591 dendritic spine membrane
GO:0042734 presynaptic membrane
GO:0042995 cell projection
GO:0043198 dendritic shaft
GO:0043679 axon terminus
GO:0044327 dendritic spine head
GO:0045095 keratin filament
GO:0045202 synapse
GO:0048471 perinuclear region of cytoplasm
GO:0048786 presynaptic active zone
GO:0055037 recycling endosome


-  Descriptions from all associated GenBank mRNAs
  AK291590 - Homo sapiens cDNA FLJ78022 complete cds, highly similar to Homo sapiens flow-induced endothelial G protein-coupled receptor (FEG-1) mRNA.
AF015257 - Homo sapiens flow-induced endothelial G protein-coupled receptor (FEG-1) mRNA, complete cds.
AK289588 - Homo sapiens cDNA FLJ75485 complete cds, highly similar to Homo sapiens G protein-coupled receptor 30 (GPR30), transcript variant 2, mRNA.
U63917 - Human G protein coupled receptor (GPCR-Br) mRNA, complete cds.
BC067849 - Homo sapiens G protein-coupled estrogen receptor 1, mRNA (cDNA clone IMAGE:5302009), with apparent retained intron.
JD175411 - Sequence 156435 from Patent EP1572962.
JD151826 - Sequence 132850 from Patent EP1572962.
JD365056 - Sequence 346080 from Patent EP1572962.
X98510 - H.sapiens mRNA for G protein-coupled receptor.
JD320573 - Sequence 301597 from Patent EP1572962.
JD041111 - Sequence 22135 from Patent EP1572962.
JD255898 - Sequence 236922 from Patent EP1572962.
BC082766 - Homo sapiens G protein-coupled estrogen receptor 1, mRNA (cDNA clone MGC:99678 IMAGE:5419168), complete cds.
BC011634 - Homo sapiens G protein-coupled estrogen receptor 1, mRNA (cDNA clone MGC:13268 IMAGE:4079700), complete cds.
JD065281 - Sequence 46305 from Patent EP1572962.
AB385528 - Synthetic construct DNA, clone: pF1KB3698, Homo sapiens GPER gene for chemokine receptor-like 2, complete cds, without stop codon, in Flexi system.
DQ895088 - Synthetic construct Homo sapiens clone IMAGE:100009548; FLH181260.01L; RZPDo839B07135D G protein-coupled receptor 30 (GPR30) gene, encodes complete protein.
KJ896924 - Synthetic construct Homo sapiens clone ccsbBroadEn_06318 GPER gene, encodes complete protein.
DQ891902 - Synthetic construct clone IMAGE:100004532; FLH181264.01X; RZPDo839B07136D G protein-coupled receptor 30 (GPR30) gene, encodes complete protein.
AB451427 - Homo sapiens GPER mRNA for G protein-coupled receptor 30, partial cds, clone: FLJ08141AAAF.
CR541655 - Homo sapiens full open reading frame cDNA clone RZPDo834D0827D for gene GPR30, G protein-coupled receptor 30; complete cds, without stopcodon.
U58828 - Human IL8-related receptor (DRY12) mRNA, complete cds.
CR541741 - Homo sapiens full open reading frame cDNA clone RZPDo834H0429D for gene GPR30, G protein-coupled receptor 30; complete cds, incl. stopcodon.
Y08162 - H.sapiens mRNA for heptahelix receptor.
JD223632 - Sequence 204656 from Patent EP1572962.
JD142160 - Sequence 123184 from Patent EP1572962.
AY265807 - Homo sapiens LERGU mRNA, complete cds.
AY265808 - Homo sapiens LERGU1 mRNA, complete cds.
AY265809 - Homo sapiens LERGU2 mRNA, complete cds.
JD487350 - Sequence 468374 from Patent EP1572962.
JD150374 - Sequence 131398 from Patent EP1572962.
JD405029 - Sequence 386053 from Patent EP1572962.
JD380198 - Sequence 361222 from Patent EP1572962.
JD095176 - Sequence 76200 from Patent EP1572962.
JD422794 - Sequence 403818 from Patent EP1572962.
JD155715 - Sequence 136739 from Patent EP1572962.
JD531307 - Sequence 512331 from Patent EP1572962.
JD434868 - Sequence 415892 from Patent EP1572962.
JD451935 - Sequence 432959 from Patent EP1572962.
JD251558 - Sequence 232582 from Patent EP1572962.
JD377115 - Sequence 358139 from Patent EP1572962.
JD210480 - Sequence 191504 from Patent EP1572962.
JD527929 - Sequence 508953 from Patent EP1572962.
JD383003 - Sequence 364027 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q99527 (Reactome details) participates in the following event(s):

R-HSA-374207 G protein-coupled estrogen receptor 1 binds estrogen non-classically
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K6C5, B5BUJ1, CEPR, CMKRL2, DRY12, ENST00000397088.1, ENST00000397088.2, ENST00000397088.3, GPER, GPER1 , GPER1_HUMAN, GPR30 , NM_001098201, O00143, O43494, Q13631, Q6FHL1, Q96F42, Q99527, Q99981, uc318yss.1, uc318yss.2
UCSC ID: ENST00000397088.4_6
RefSeq Accession: NM_001098201.3
Protein: Q99527 (aka GPER_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.