ID:GORS1_HUMAN DESCRIPTION: RecName: Full=Golgi reassembly-stacking protein 1; AltName: Full=Golgi peripheral membrane protein p65; AltName: Full=Golgi phosphoprotein 5; Short=GOLPH5; AltName: Full=Golgi reassembly-stacking protein of 65 kDa; Short=GRASP65; FUNCTION: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. SUBUNIT: Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115. Interacts with TMED2 and TMED3 (By similarity). SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Note=Undergoes rapid exchange with the cytosol. PTM: Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking. PTM: Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity). SIMILARITY: Belongs to the GORASP family. SIMILARITY: Contains 1 PDZ (DHR) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9BQQ3
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006487 protein N-linked glycosylation GO:0006888 ER to Golgi vesicle-mediated transport GO:0007030 Golgi organization GO:0015031 protein transport GO:0048208 COPII vesicle coating GO:0050774 negative regulation of dendrite morphogenesis GO:1904668 positive regulation of ubiquitin protein ligase activity
LF208665 - JP 2014500723-A/16168: Polycomb-Associated Non-Coding RNAs. JD289734 - Sequence 270758 from Patent EP1572962. AK027096 - Homo sapiens cDNA: FLJ23443 fis, clone HSI01206. AK001574 - Homo sapiens cDNA FLJ10712 fis, clone NT2RP3000919, highly similar to Rattus norvegicus golgi peripheral membrane protein p65 mRNA. AJ409349 - Homo sapiens mRNA for golgi perepheral membrane protein p65 (GRASP65 gene). AK124755 - Homo sapiens cDNA FLJ42765 fis, clone BRAWH3002853, highly similar to Golgi reassembly-stacking protein 1. AK091168 - Homo sapiens cDNA FLJ33849 fis, clone CTONG2005585, moderately similar to Rattus norvegicus golgi peripheral membrane protein p65 (GRASP65) mRNA. AX746824 - Sequence 349 from Patent EP1308459. BC064624 - Homo sapiens golgi reassembly stacking protein 1, 65kDa, mRNA (cDNA clone IMAGE:5743844), partial cds. BC008928 - Homo sapiens golgi reassembly stacking protein 1, 65kDa, mRNA (cDNA clone IMAGE:3840718), complete cds. AK057043 - Homo sapiens cDNA FLJ32481 fis, clone SKNMC2001113, moderately similar to Golgi reassembly-stacking protein 1. BC075854 - Homo sapiens golgi reassembly stacking protein 1, 65kDa, mRNA (cDNA clone MGC:90260 IMAGE:5503263), complete cds. JD198691 - Sequence 179715 from Patent EP1572962. JD339387 - Sequence 320411 from Patent EP1572962. JD448422 - Sequence 429446 from Patent EP1572962. BC103826 - Homo sapiens golgi reassembly stacking protein 1, 65kDa, mRNA (cDNA clone MGC:118894 IMAGE:40001584), complete cds. BC103827 - Homo sapiens golgi reassembly stacking protein 1, 65kDa, mRNA (cDNA clone MGC:118897 IMAGE:40001588), complete cds. JD067027 - Sequence 48051 from Patent EP1572962. JD355238 - Sequence 336262 from Patent EP1572962. JD075796 - Sequence 56820 from Patent EP1572962. JD241853 - Sequence 222877 from Patent EP1572962. JD552128 - Sequence 533152 from Patent EP1572962. JD499320 - Sequence 480344 from Patent EP1572962. JD344518 - Sequence 325542 from Patent EP1572962. JD242444 - Sequence 223468 from Patent EP1572962. JD384634 - Sequence 365658 from Patent EP1572962. JD073838 - Sequence 54862 from Patent EP1572962. JD177737 - Sequence 158761 from Patent EP1572962. JD471185 - Sequence 452209 from Patent EP1572962. JD284605 - Sequence 265629 from Patent EP1572962. JD485288 - Sequence 466312 from Patent EP1572962. JD121067 - Sequence 102091 from Patent EP1572962. JD349664 - Sequence 330688 from Patent EP1572962. DQ594777 - Homo sapiens piRNA piR-60889, complete sequence. JD382211 - Sequence 363235 from Patent EP1572962. JD114663 - Sequence 95687 from Patent EP1572962. LF378867 - JP 2014500723-A/186370: Polycomb-Associated Non-Coding RNAs. JD098484 - Sequence 79508 from Patent EP1572962. JD346205 - Sequence 327229 from Patent EP1572962. JD226655 - Sequence 207679 from Patent EP1572962. JD133283 - Sequence 114307 from Patent EP1572962. JD342965 - Sequence 323989 from Patent EP1572962. JD254993 - Sequence 236017 from Patent EP1572962. JD446634 - Sequence 427658 from Patent EP1572962. JD436538 - Sequence 417562 from Patent EP1572962. JD495020 - Sequence 476044 from Patent EP1572962. JD368064 - Sequence 349088 from Patent EP1572962. JD493469 - Sequence 474493 from Patent EP1572962. JD374002 - Sequence 355026 from Patent EP1572962. AK303847 - Homo sapiens cDNA FLJ58222 complete cds, highly similar to Golgi reassembly-stacking protein 1. JD206654 - Sequence 187678 from Patent EP1572962. JD214986 - Sequence 196010 from Patent EP1572962. JD249435 - Sequence 230459 from Patent EP1572962. JD075616 - Sequence 56640 from Patent EP1572962. HQ447446 - Synthetic construct Homo sapiens clone IMAGE:100070774; CCSB014345_01 golgi reassembly stacking protein 1, 65kDa (GORASP1) gene, encodes complete protein. KJ894565 - Synthetic construct Homo sapiens clone ccsbBroadEn_03959 GORASP1 gene, encodes complete protein. AB527597 - Synthetic construct DNA, clone: pF1KB6632, Homo sapiens GORASP1 gene for golgi reassembly stacking protein 1, 65kDa, without stop codon, in Flexi system. CU687304 - Synthetic construct Homo sapiens gateway clone IMAGE:100022846 5' read GORASP1 mRNA. AK300271 - Homo sapiens cDNA FLJ58423 complete cds, highly similar to Golgi reassembly-stacking protein 1. KJ906299 - Synthetic construct Homo sapiens clone ccsbBroadEn_15969 GORASP1 gene, encodes complete protein. JD225594 - Sequence 206618 from Patent EP1572962. MA444242 - JP 2018138019-A/16168: Polycomb-Associated Non-Coding RNAs. MA614444 - JP 2018138019-A/186370: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9BQQ3 (Reactome details) participates in the following event(s):
R-HSA-2172183 Phosphorylation of GORASP1, GOLGA2 and RAB1A by CDK1:CCNB R-HSA-5694418 RAB1:GTP binds USO1 and GORASP1:GOLGA2 R-HSA-5694446 BET1:GOSR2:STX5 bind v-SNARES on tethered vesicle R-HSA-2172194 Recruitment of PLK1 to phosphorylated GORASP1 (GRASP65) R-HSA-2214351 PLK1 phosphorylates GORASP1 R-HSA-6809006 Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex R-HSA-5694441 CSNK1D phosphorylates SEC23 R-HSA-6809011 cis-Golgi t-SNAREs bind YKT6 on tethered vesicle R-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization R-HSA-204005 COPII-mediated vesicle transport R-HSA-6807878 COPI-mediated anterograde transport R-HSA-68875 Mitotic Prophase R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-68886 M Phase R-HSA-199991 Membrane Trafficking R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-69278 Cell Cycle (Mitotic) R-HSA-5653656 Vesicle-mediated transport R-HSA-446203 Asparagine N-linked glycosylation R-HSA-1640170 Cell Cycle R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
