Human Gene GATAD2A (ENST00000683918.1_2) from GENCODE V47lift37
  Description: GATA zinc finger domain containing 2A, transcript variant 33 (from RefSeq NM_001384541.1)
Gencode Transcript: ENST00000683918.1_2
Gencode Gene: ENSG00000167491.18_16
Transcript (Including UTRs)
   Position: hg19 chr19:19,516,484-19,619,741 Size: 103,258 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr19:19,576,155-19,616,283 Size: 40,129 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:19,516,484-19,619,741)mRNA (may differ from genome)Protein (634 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: P66A_HUMAN
DESCRIPTION: RecName: Full=Transcriptional repressor p66-alpha; Short=Hp66alpha; AltName: Full=GATA zinc finger domain-containing protein 2A;
FUNCTION: Transcriptional repressor.
SUBUNIT: Binds MBD2 and MBD3. Part of a complex containing MBD2, HDAC1 and/or HDAC2.
SUBCELLULAR LOCATION: Nucleus speckle.
TISSUE SPECIFICITY: Ubiquitous, both in fetal and adult tissues.
SIMILARITY: Contains 1 GATA-type zinc finger.
SEQUENCE CAUTION: Sequence=AAH11684.1; Type=Erroneous initiation; Sequence=BAA90939.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -216.50351-0.617 Picture PostScript Text
3' UTR -1240.903458-0.359 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000679 - Znf_GATA
IPR013088 - Znf_NHR/GATA

Pfam Domains:
PF00320 - GATA zinc finger
PF16563 - Coiled-coil and interaction region of P66A and P66B with MBD2

SCOP Domains:
57716 - Glucocorticoid receptor-like (DNA-binding domain)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2L2L - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q86YP4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0030674 protein binding, bridging
GO:0043565 sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006306 DNA methylation
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0045892 negative regulation of transcription, DNA-templated

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016581 NuRD complex
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  AK303482 - Homo sapiens cDNA FLJ60209 complete cds, highly similar to Transcriptional repressor p66 alpha.
AK125974 - Homo sapiens cDNA FLJ43986 fis, clone TESTI4019140, moderately similar to Xenopus laevis Mi-2 histone deacetylase complex protein 66.
BC011684 - Homo sapiens GATA zinc finger domain containing 2A, mRNA (cDNA clone MGC:19816 IMAGE:3953055), complete cds.
AB463677 - Synthetic construct DNA, clone: pF1KB6140, Homo sapiens GATAD2A gene for GATA zinc finger domain containing 2A, without stop codon, in Flexi system.
AY186731 - Homo sapiens p66 alpha mRNA, complete cds.
JD339234 - Sequence 320258 from Patent EP1572962.
AK315416 - Homo sapiens cDNA, FLJ96467.
JD173522 - Sequence 154546 from Patent EP1572962.
AK296781 - Homo sapiens cDNA FLJ55704 complete cds, highly similar to Transcriptional repressor p66 alpha.
JD208247 - Sequence 189271 from Patent EP1572962.
JD189627 - Sequence 170651 from Patent EP1572962.
JD456156 - Sequence 437180 from Patent EP1572962.
JD355538 - Sequence 336562 from Patent EP1572962.
JD276029 - Sequence 257053 from Patent EP1572962.
EU446496 - Synthetic construct Homo sapiens clone IMAGE:100070115; IMAGE:100011705; FLH258155.01L GATA zinc finger domain containing 2A (GATAD2A) gene, encodes complete protein.
KJ902694 - Synthetic construct Homo sapiens clone ccsbBroadEn_12088 GATAD2A gene, encodes complete protein.
BC012902 - Homo sapiens GATA zinc finger domain containing 2A, mRNA (cDNA clone IMAGE:3864801), partial cds.
AK000092 - Homo sapiens cDNA FLJ20085 fis, clone COL03604.
AK225198 - Homo sapiens mRNA for GATA zinc finger domain containing 2A variant, clone: COL03604.
AL390164 - Homo sapiens mRNA; cDNA DKFZp547D147 (from clone DKFZp547D147).
DQ573509 - Homo sapiens piRNA piR-41621, complete sequence.
JD339133 - Sequence 320157 from Patent EP1572962.
JD259430 - Sequence 240454 from Patent EP1572962.
JD137610 - Sequence 118634 from Patent EP1572962.
JD067991 - Sequence 49015 from Patent EP1572962.
JD102243 - Sequence 83267 from Patent EP1572962.
JD279633 - Sequence 260657 from Patent EP1572962.
JD057863 - Sequence 38887 from Patent EP1572962.
JD241965 - Sequence 222989 from Patent EP1572962.
JD060107 - Sequence 41131 from Patent EP1572962.
JD229499 - Sequence 210523 from Patent EP1572962.
JD535072 - Sequence 516096 from Patent EP1572962.
JD136755 - Sequence 117779 from Patent EP1572962.
JD223151 - Sequence 204175 from Patent EP1572962.
JD278301 - Sequence 259325 from Patent EP1572962.
JD546337 - Sequence 527361 from Patent EP1572962.
DQ592779 - Homo sapiens piRNA piR-59891, complete sequence.
JD147229 - Sequence 128253 from Patent EP1572962.
JD289106 - Sequence 270130 from Patent EP1572962.
JD449649 - Sequence 430673 from Patent EP1572962.
JD271762 - Sequence 252786 from Patent EP1572962.
JD373976 - Sequence 355000 from Patent EP1572962.
JD443494 - Sequence 424518 from Patent EP1572962.
JD182800 - Sequence 163824 from Patent EP1572962.
JD041799 - Sequence 22823 from Patent EP1572962.
JD261815 - Sequence 242839 from Patent EP1572962.
JD114551 - Sequence 95575 from Patent EP1572962.
JD429860 - Sequence 410884 from Patent EP1572962.
JD423392 - Sequence 404416 from Patent EP1572962.
AK024670 - Homo sapiens cDNA: FLJ21017 fis, clone CAE05907.
BC009034 - Homo sapiens cDNA clone IMAGE:4177549, partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q86YP4 (Reactome details) participates in the following event(s):

R-HSA-427404 Recruitment of ERCC6 (CSB), EHMT2 (G9a), and NuRD to the promoter of rRNA gene
R-HSA-8943780 SALL4 recruits NuRD to PTEN gene
R-HSA-6805650 MTA2-NuRD complex deacetylates TP53
R-HSA-427336 TTF1:rRNA promoter:ERCC6:EHMT2 complex dimethylates histone H3 at lysine-9.
R-HSA-3769447 HDAC1/2 containing-complexes deacetylate histones
R-HSA-427389 ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression
R-HSA-8943724 Regulation of PTEN gene transcription
R-HSA-5250913 Positive epigenetic regulation of rRNA expression
R-HSA-6807070 PTEN Regulation
R-HSA-6804758 Regulation of TP53 Activity through Acetylation
R-HSA-3214815 HDACs deacetylate histones
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-5633007 Regulation of TP53 Activity
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-74160 Gene expression (Transcription)
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-4839726 Chromatin organization
R-HSA-162582 Signal Transduction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription

-  Other Names for This Gene
  Alternate Gene Symbols: B5MC40, NM_001384541, P66A_HUMAN, Q7L3J2, Q86YP4, Q96F28, Q9NPU2, Q9NXS1, uc330mck.1, uc330mck.2
UCSC ID: ENST00000683918.1_2
RefSeq Accession: NM_001384528.1
Protein: Q86YP4 (aka P66A_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
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