Human Gene FARSB (ENST00000281828.8_8) from GENCODE V47lift37

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Description: phenylalanyl-tRNA synthetase subunit beta, transcript variant 2 (from RefSeq NR_130154.2)
Gencode Transcript: ENST00000281828.8_8
Gencode Gene: ENSG00000116120.11_10
Transcript (Including UTRs)
Position: hg19 chr2:223,431,618-223,520,811 Size: 89,194 Total Exon Count: 17 Strand: -
Coding Region
Position: hg19 chr2:223,436,590-223,520,792 Size: 84,203 Coding Exon Count: 17

Page Index	Sequence and Links	UniProtKB Comments	Primers	MalaCards	CTD
Gene Alleles	RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species
GO Annotations	mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr2:223,431,618-223,520,811)			mRNA (may differ from genome)		Protein (589 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	Malacards	MGI	OMIM	PubMed	Reactome
UniProtKB	Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: SYFB_HUMAN DESCRIPTION: RecName: Full=Phenylalanine--tRNA ligase beta subunit; EC=6.1.1.20; AltName: Full=Phenylalanyl-tRNA synthetase beta subunit; Short=PheRS; CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). SUBUNIT: Tetramer of two alpha and two beta subunits (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). SIMILARITY: Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily. SIMILARITY: Contains 1 B5 domain.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

MalaCards Disease Associations


	MalaCards Gene Search: FARSB Diseases sorted by gene-association score: muscular dystrophy, rigid spine, 1 (1)

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl C016403 2,4-dinitrotoluene C023514 2,6-dinitrotoluene C009505 4,4'-diaminodiphenylmethane C035207 4-amino-2,6-dinitrotoluene C075773 4-phenylbutyric acid D001564 Benzo(a)pyrene D002995 Clofibric Acid D019327 Copper Sulfate D003375 Coumestrol more ... click here to view the complete list

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 25.62 RPKM in Cells - EBV-transformed lymphocytes Total median expression: 565.96 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-0.90	19	-0.047	Picture	PostScript	Text
3' UTR	-1546.80	4972	-0.311	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR005146 - B3/B4_tRNA-bd
IPR009061 - DNA-bd_dom_put
IPR004531 - Phe-tRNA-synth_IIc_bsu_arc
IPR020825 - Phe-tRNA_synthase_B3/B4
IPR005147 - tRNA_synthase_B5-dom

Pfam Domains:
PF03483 - B3/4 domain
PF03484 - tRNA synthetase B5 domain
PF17759 - Phenylalanyl tRNA synthetase beta chain CLM domain
PF18262 - Phe-tRNA synthetase beta subunit B1 domain

SCOP Domains:
46955 - Putative DNA-binding domain
56037 - PheT/TilS domain
51338 - Composite domain of metallo-dependent hydrolases
55681 - Class II aaRS and biotin synthetases

Protein Data Bank (PDB) 3-D Structure
MuPIT help

3L4G - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on Q9NSD9


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	Genome Browser	Genome Browser
Gene Details	Gene Details		Gene Details	Gene Details	Gene Details
Gene Sorter	Gene Sorter		Gene Sorter	Gene Sorter	Gene Sorter
	RGD	Ensembl		WormBase	SGD
				Protein Sequence	Protein Sequence
				Alignment	Alignment

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0000166 nucleotide binding GO:0000287 magnesium ion binding GO:0003723 RNA binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004826 phenylalanine-tRNA ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016874 ligase activity Biological Process: GO:0006412 translation GO:0006432 phenylalanyl-tRNA aminoacylation GO:0051290 protein heterotetramerization Cellular Component: GO:0005737 cytoplasm GO:0005829 cytosol GO:0009328 phenylalanine-tRNA ligase complex GO:0016020 membrane

Descriptions from all associated GenBank mRNAs


	D84430 - Homo sapiens mRNA for phenylalanyl tRNA synthetase, complete cds. AF042346 - Homo sapiens putative phenylalanyl-tRNA synthetase beta-subunit mRNA, complete cds. AK055289 - Homo sapiens cDNA FLJ30727 fis, clone FEBRA2000007, highly similar to Homo sapiens putative phenylalanyl-tRNA synthetase beta-subunit mRNA. AK294158 - Homo sapiens cDNA FLJ57266 complete cds, highly similar to Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20). BC006502 - Homo sapiens phenylalanyl-tRNA synthetase, beta subunit, mRNA (cDNA clone IMAGE:2820530), partial cds. AK291531 - Homo sapiens cDNA FLJ75460 complete cds, highly similar to Homo sapiens phenylalanine-tRNA synthetase-like, beta subunit, mRNA. AF161521 - Homo sapiens HSPC173 mRNA, complete cds. BC008434 - Homo sapiens cDNA clone IMAGE:4078272, containing frame-shift errors. BC017783 - Homo sapiens phenylalanyl-tRNA synthetase, beta subunit, mRNA (cDNA clone MGC:22277 IMAGE:4664949), complete cds. BC022273 - Homo sapiens phenylalanyl-tRNA synthetase, beta subunit, mRNA (cDNA clone IMAGE:4663369), containing frame-shift errors. AK316497 - Homo sapiens cDNA, FLJ79396 complete cds, highly similar to Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20). KJ898147 - Synthetic construct Homo sapiens clone ccsbBroadEn_07541 FARSB gene, encodes complete protein. AK090760 - Homo sapiens cDNA FLJ33441 fis, clone BRACE2021932. JD173762 - Sequence 154786 from Patent EP1572962. JD205861 - Sequence 186885 from Patent EP1572962. JD040943 - Sequence 21967 from Patent EP1572962. JD169028 - Sequence 150052 from Patent EP1572962. JD529043 - Sequence 510067 from Patent EP1572962. JD557277 - Sequence 538301 from Patent EP1572962. JD261663 - Sequence 242687 from Patent EP1572962. JD118445 - Sequence 99469 from Patent EP1572962. JD169027 - Sequence 150051 from Patent EP1572962. JD375190 - Sequence 356214 from Patent EP1572962. JD134806 - Sequence 115830 from Patent EP1572962. JD278185 - Sequence 259209 from Patent EP1572962. JD498504 - Sequence 479528 from Patent EP1572962. JD075224 - Sequence 56248 from Patent EP1572962. JD377675 - Sequence 358699 from Patent EP1572962. JD522508 - Sequence 503532 from Patent EP1572962. JD289033 - Sequence 270057 from Patent EP1572962. JD046713 - Sequence 27737 from Patent EP1572962. JD474488 - Sequence 455512 from Patent EP1572962. JD220312 - Sequence 201336 from Patent EP1572962. JD544985 - Sequence 526009 from Patent EP1572962. JD311028 - Sequence 292052 from Patent EP1572962. JD524248 - Sequence 505272 from Patent EP1572962. JD270030 - Sequence 251054 from Patent EP1572962. JD202212 - Sequence 183236 from Patent EP1572962. JD207731 - Sequence 188755 from Patent EP1572962. JD465818 - Sequence 446842 from Patent EP1572962. JD232838 - Sequence 213862 from Patent EP1572962. JD427825 - Sequence 408849 from Patent EP1572962. JD115123 - Sequence 96147 from Patent EP1572962. JD223552 - Sequence 204576 from Patent EP1572962. CU676064 - Synthetic construct Homo sapiens gateway clone IMAGE:100022300 5' read FARSB mRNA. BC011261 - Homo sapiens phenylalanyl-tRNA synthetase, beta subunit, mRNA (cDNA clone IMAGE:4178758), containing frame-shift errors. AK001025 - Homo sapiens cDNA FLJ10163 fis, clone HEMBA1003568, weakly similar to 52 KD RO PROTEIN.

Biochemical and Signaling Pathways


	BioCyc Knowledge Library TRNA-CHARGING-PWY - tRNA charging Reactome (by CSHL, EBI, and GO) Protein Q9NSD9 (Reactome details) participates in the following event(s): R-HSA-379848 phenylalanine + tRNA(Phe) + ATP => Phe-tRNA(Phe) + AMP + pyrophosphate R-HSA-379716 Cytosolic tRNA aminoacylation R-HSA-379724 tRNA Aminoacylation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins

Other Names for This Gene


	Alternate Gene Symbols: B4DFM0, ENST00000281828.1, ENST00000281828.2, ENST00000281828.3, ENST00000281828.4, ENST00000281828.5, ENST00000281828.6, ENST00000281828.7, FARSLB, FRSB, HSPC173, NR_130154, O95708, Q4ZFX1, Q57ZJ5, Q9NSD9, Q9NZZ6, SYFB_HUMAN, uc317jxj.1, uc317jxj.2 UCSC ID: ENST00000281828.8_8 RefSeq Accession: NM_005687.5 Protein: Q9NSD9 (aka SYFB_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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