Human Gene FAM32A (ENST00000263384.12_4) from GENCODE V47lift37
  Description: family with sequence similarity 32 member A (from RefSeq NM_014077.4)
Gencode Transcript: ENST00000263384.12_4
Gencode Gene: ENSG00000105058.12_8
Transcript (Including UTRs)
   Position: hg19 chr19:16,296,221-16,302,857 Size: 6,637 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr19:16,296,254-16,301,766 Size: 5,513 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:16,296,221-16,302,857)mRNA (may differ from genome)Protein (112 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FA32A_HUMAN
DESCRIPTION: RecName: Full=Protein FAM32A; AltName: Full=Ovarian tumor-associated gene 12; Short=OTAG-12;
FUNCTION: Isoform 1, but not isoform 2 or isoform 3, may induce G2 arrest and apoptosis. May also increase cell sensitivity to apoptotic stimuli.
SUBCELLULAR LOCATION: Isoform 1: Nucleus.
SUBCELLULAR LOCATION: Isoform 2: Nucleus.
TISSUE SPECIFICITY: Expressed in ovary, with isoform 1 being predominant.
INDUCTION: By etoposide, puromycin or carboplatin.
SIMILARITY: Belongs to the FAM32 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 42.03 RPKM in Spleen
Total median expression: 1565.20 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.8033-0.297 Picture PostScript Text
3' UTR -350.601091-0.321 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013865 - DUF1754_euk

Pfam Domains:
PF08555 - FAM32A

ModBase Predicted Comparative 3D Structure on Q9Y421
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding

Biological Process:
GO:0006915 apoptotic process
GO:0007049 cell cycle
GO:0008150 biological_process

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus


-  Descriptions from all associated GenBank mRNAs
  AK312058 - Homo sapiens cDNA, FLJ92339.
BC000639 - Homo sapiens family with sequence similarity 32, member A, mRNA (cDNA clone MGC:1450 IMAGE:3345441), complete cds.
AL050157 - Homo sapiens mRNA; cDNA DKFZp586O0120 (from clone DKFZp586O0120).
BC017286 - Homo sapiens family with sequence similarity 32, member A, mRNA (cDNA clone MGC:29653 IMAGE:4892079), complete cds.
AM393549 - Synthetic construct Homo sapiens clone IMAGE:100001828 for hypothetical protein (FAM32A gene).
AM393669 - Synthetic construct Homo sapiens clone IMAGE:100001837 for hypothetical protein (FAM32A gene).
KJ893510 - Synthetic construct Homo sapiens clone ccsbBroadEn_02904 FAM32A gene, encodes complete protein.
AF151902 - Homo sapiens CGI-144 protein mRNA, complete cds.
JD462180 - Sequence 443204 from Patent EP1572962.
BC004447 - Homo sapiens family with sequence similarity 32, member A, mRNA (cDNA clone IMAGE:2819208), partial cds.
JD350411 - Sequence 331435 from Patent EP1572962.
JD099038 - Sequence 80062 from Patent EP1572962.
JD403063 - Sequence 384087 from Patent EP1572962.
JD316831 - Sequence 297855 from Patent EP1572962.
JD563554 - Sequence 544578 from Patent EP1572962.
JD033278 - Sequence 14302 from Patent EP1572962.
JD120190 - Sequence 101214 from Patent EP1572962.
JD077759 - Sequence 58783 from Patent EP1572962.
JD287662 - Sequence 268686 from Patent EP1572962.
JD189724 - Sequence 170748 from Patent EP1572962.
JD219193 - Sequence 200217 from Patent EP1572962.
JD307506 - Sequence 288530 from Patent EP1572962.
JD203284 - Sequence 184308 from Patent EP1572962.
JD141463 - Sequence 122487 from Patent EP1572962.
JD077654 - Sequence 58678 from Patent EP1572962.
JD317879 - Sequence 298903 from Patent EP1572962.
JD186307 - Sequence 167331 from Patent EP1572962.
JD063632 - Sequence 44656 from Patent EP1572962.
JD234543 - Sequence 215567 from Patent EP1572962.
JD245676 - Sequence 226700 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: CGI-144, ENST00000263384.1, ENST00000263384.10, ENST00000263384.11, ENST00000263384.2, ENST00000263384.3, ENST00000263384.4, ENST00000263384.5, ENST00000263384.6, ENST00000263384.7, ENST00000263384.8, ENST00000263384.9, FA32A_HUMAN, NM_014077, OTAG12, Q9BT02, Q9Y421, uc317hjl.1, uc317hjl.2
UCSC ID: ENST00000263384.12_4
RefSeq Accession: NM_014077.4
Protein: Q9Y421 (aka FA32A_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.