Human Gene DNAJA4 (ENST00000343789.7_5) from GENCODE V47lift37
  Description: DnaJ heat shock protein family (Hsp40) member A4, transcript variant 8 (from RefSeq NM_001387388.1)
Gencode Transcript: ENST00000343789.7_5
Gencode Gene: ENSG00000140403.13_9
Transcript (Including UTRs)
   Position: hg19 chr15:78,556,904-78,574,538 Size: 17,635 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr15:78,557,106-78,572,802 Size: 15,697 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:78,556,904-78,574,538)mRNA (may differ from genome)Protein (397 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MGIPubMedReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: DNJA4_HUMAN
DESCRIPTION: RecName: Full=DnaJ homolog subfamily A member 4; Flags: Precursor;
SUBCELLULAR LOCATION: Membrane; Lipid-anchor (Potential).
SIMILARITY: Contains 1 CR-type zinc finger.
SIMILARITY: Contains 1 J domain.
SEQUENCE CAUTION: Sequence=AAH21720.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D000082 Acetaminophen
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • D015058 1-Naphthylisothiocyanate
  • C016403 2,4-dinitrotoluene
  • C023514 2,6-dinitrotoluene
  • C532162 2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine
  • C009505 4,4'-diaminodiphenylmethane
  • D016604 Aflatoxin B1
  • D001564 Benzo(a)pyrene
  • D019256 Cadmium Chloride
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 62.06 RPKM in Testis
Total median expression: 905.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -6.4031-0.206 Picture PostScript Text
3' UTR -507.101736-0.292 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012724 - DnaJ
IPR002939 - DnaJ_C
IPR001623 - DnaJ_N
IPR018253 - Heat_shock_DnaJ_CS
IPR008971 - HSP40/DnaJ_pept-bd
IPR003095 - Hsp_DnaJ
IPR001305 - HSP_DnaJ_Cys-rich_dom

Pfam Domains:
PF00226 - DnaJ domain
PF00684 - DnaJ central domain
PF01556 - DnaJ C terminal domain

SCOP Domains:
46565 - Chaperone J-domain
49493 - HSP40/DnaJ peptide-binding domain
57938 - DnaJ/Hsp40 cysteine-rich domain

ModBase Predicted Comparative 3D Structure on Q8WW22
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0031072 heat shock protein binding
GO:0046872 metal ion binding
GO:0051082 unfolded protein binding
GO:0051087 chaperone binding

Biological Process:
GO:0006457 protein folding
GO:0009408 response to heat
GO:0042026 protein refolding
GO:0090084 negative regulation of inclusion body assembly

Cellular Component:
GO:0005829 cytosol
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BC021720 - Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 4, mRNA (cDNA clone MGC:33001 IMAGE:4831235), complete cds.
HZ466378 - JP 2015532642-A/2: MicroRNAs that Suppress Human Melanoma.
LP722490 - Sequence 3 from Patent EP2882496.
AK098079 - Homo sapiens cDNA FLJ40760 fis, clone TRACH2002803, highly similar to Mus musculus mmDNAJA4 mRNA for mmDj4.
BX648710 - Homo sapiens mRNA; cDNA DKFZp686G2074 (from clone DKFZp686G2074).
AK096616 - Homo sapiens cDNA FLJ39297 fis, clone OCBBF2012812, highly similar to Mus musculus mmDNAJA4 mRNA for mmDj4.
HZ466379 - JP 2015532642-A/3: MicroRNAs that Suppress Human Melanoma.
LP722492 - Sequence 5 from Patent EP2882496.
KJ904805 - Synthetic construct Homo sapiens clone ccsbBroadEn_14199 DNAJA4-like gene, encodes complete protein.
BC031044 - Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 4, mRNA (cDNA clone MGC:33002 IMAGE:5277834), complete cds.
HZ466380 - JP 2015532642-A/4: MicroRNAs that Suppress Human Melanoma.
LP722494 - Sequence 7 from Patent EP2882496.
AF116663 - Homo sapiens PRO1472 mRNA, complete cds.
JD475511 - Sequence 456535 from Patent EP1572962.
AK090898 - Homo sapiens cDNA FLJ33579 fis, clone BRAMY2011767, highly similar to Mus musculus mmDNAJA4 mRNA for mmDj4.
AX746667 - Sequence 192 from Patent EP1308459.
KJ906227 - Synthetic construct Homo sapiens clone ccsbBroadEn_15897 DNAJA4 gene, encodes complete protein.
AF173388 - Homo sapiens MSTP104 (MST104) mRNA, complete cds.
AL133096 - Homo sapiens mRNA; cDNA DKFZp434N1728 (from clone DKFZp434N1728).
AL133434 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1639909.
AL133433 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1640784.
AK025069 - Homo sapiens cDNA: FLJ21416 fis, clone COL04034.
AL133431 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 730912.
AL133432 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 193464.
MA622443 - JP 2018140982-A/2: MicroRNAs that Suppress Human Melanoma.
MA622444 - JP 2018140982-A/3: MicroRNAs that Suppress Human Melanoma.
MA622445 - JP 2018140982-A/4: MicroRNAs that Suppress Human Melanoma.
MP557054 - Sequence 3 from Patent EP3626309.
MP557056 - Sequence 5 from Patent EP3626309.
MP557058 - Sequence 7 from Patent EP3626309.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8WW22 (Reactome details) participates in the following event(s):

R-HSA-3371503 STIP1(HOP) binds HSP90 and HSP70:HSP40:nascent protein
R-HSA-3371422 ATP hydrolysis by HSP70
R-HSA-3371590 HSP70 binds to HSP40:nascent protein
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-2262752 Cellular responses to stress
R-HSA-8953897 Cellular responses to external stimuli

-  Other Names for This Gene
  Alternate Gene Symbols: DNJA4_HUMAN, E9PDM9, ENST00000343789.1, ENST00000343789.2, ENST00000343789.3, ENST00000343789.4, ENST00000343789.5, ENST00000343789.6, NM_001387388, Q6AW87, Q8N5Z4, Q8N7P2, Q8WW22, uc317wta.1, uc317wta.2
UCSC ID: ENST00000343789.7_5
RefSeq Accession: NM_001387388.1
Protein: Q8WW22 (aka DNJA4_HUMAN or DJA4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.