Human Gene CXCL1 (ENST00000395761.4_4) from GENCODE V47lift37
  Description: C-X-C motif chemokine ligand 1, transcript variant 2 (from RefSeq NR_046035.2)
Gencode Transcript: ENST00000395761.4_4
Gencode Gene: ENSG00000163739.5_7
Transcript (Including UTRs)
   Position: hg19 chr4:74,735,110-74,737,025 Size: 1,916 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr4:74,735,188-74,736,253 Size: 1,066 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:74,735,110-74,737,025)mRNA (may differ from genome)Protein (107 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GROA_HUMAN
DESCRIPTION: RecName: Full=Growth-regulated alpha protein; AltName: Full=C-X-C motif chemokine 1; AltName: Full=GRO-alpha(1-73); AltName: Full=Melanoma growth stimulatory activity; Short=MGSA; AltName: Full=Neutrophil-activating protein 3; Short=NAP-3; Contains: RecName: Full=GRO-alpha(4-73); Contains: RecName: Full=GRO-alpha(5-73); Contains: RecName: Full=GRO-alpha(6-73); Flags: Precursor;
FUNCTION: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
SUBCELLULAR LOCATION: Secreted.
PTM: N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
WEB RESOURCE: Name=Wikipedia; Note=CXCL1 entry; URL="http://en.wikipedia.org/wiki/CXCL1";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CXCL1
Diseases sorted by gene-association score: kaposi sarcoma (6), melanoma (5), rheumatoid arthritis (4), gastritis (4), meningitis (4), keratitis (3), asthma (3), acute cystitis (3), pneumonia (3), c6 deficiency (2), retinitis pigmentosa 73 (2), acute cervicitis (1), la crosse encephalitis (1), griscelli syndrome, type 1 (1), cervix small cell carcinoma (1), pulmonary fibrosis, idiopathic (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 99.13 RPKM in Spleen
Total median expression: 233.17 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.1078-0.271 Picture PostScript Text
3' UTR -195.10772-0.253 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001089 - Chemokine_CXC
IPR018048 - Chemokine_CXC_CS
IPR002473 - Chemokine_CXCL8/IL8
IPR001811 - Chemokine_IL8-like_dom

Pfam Domains:
PF00048 - Small cytokines (intecrine/chemokine), interleukin-8 like

SCOP Domains:
54117 - Interleukin 8-like chemokines

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1MGS - NMR MuPIT 1MSG - NMR MuPIT 1MSH - NMR MuPIT 1ROD - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P09341
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005125 cytokine activity
GO:0008009 chemokine activity
GO:0008047 enzyme activator activity
GO:0008083 growth factor activity
GO:0045236 CXCR chemokine receptor binding

Biological Process:
GO:0006935 chemotaxis
GO:0006952 defense response
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007399 nervous system development
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010469 regulation of receptor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0030036 actin cytoskeleton organization
GO:0031640 killing of cells of other organism
GO:0032496 response to lipopolysaccharide
GO:0035556 intracellular signal transduction
GO:0043085 positive regulation of catalytic activity
GO:0043312 neutrophil degranulation
GO:0060326 cell chemotaxis
GO:0070098 chemokine-mediated signaling pathway
GO:0090023 positive regulation of neutrophil chemotaxis

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0035580 specific granule lumen
GO:1904724 tertiary granule lumen


-  Descriptions from all associated GenBank mRNAs
  JD098071 - Sequence 79095 from Patent EP1572962.
LQ882872 - Sequence 21 from Patent WO2018160841.
LF206882 - JP 2014500723-A/14385: Polycomb-Associated Non-Coding RNAs.
LF213721 - JP 2014500723-A/21224: Polycomb-Associated Non-Coding RNAs.
BC011976 - Homo sapiens chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), mRNA (cDNA clone MGC:9049 IMAGE:3856841), complete cds.
J03561 - Human gro (growth regulated) gene.
FW340090 - Screening.
X12510 - Human mRNA for melanoma growth stimulatory activity (MGSA).
AB590217 - Synthetic construct DNA, clone: pFN21AE1416, Homo sapiens CXCL1 gene for chemokine (C-X-C motif) ligand 1, without stop codon, in Flexi system.
DQ893591 - Synthetic construct clone IMAGE:100006221; FLH179653.01X; RZPDo839A12132D chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) gene, encodes complete protein.
DQ894941 - Synthetic construct Homo sapiens clone IMAGE:100009401; FLH179649.01L; RZPDo839A12131D chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) gene, encodes complete protein.
KJ891301 - Synthetic construct Homo sapiens clone ccsbBroadEn_00695 CXCL1 gene, encodes complete protein.
BT006880 - Homo sapiens chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) mRNA, complete cds.
LF358957 - JP 2014500723-A/166460: Polycomb-Associated Non-Coding RNAs.
LF358958 - JP 2014500723-A/166461: Polycomb-Associated Non-Coding RNAs.
JD247653 - Sequence 228677 from Patent EP1572962.
JD553627 - Sequence 534651 from Patent EP1572962.
JD073547 - Sequence 54571 from Patent EP1572962.
JD411666 - Sequence 392690 from Patent EP1572962.
JD070876 - Sequence 51900 from Patent EP1572962.
MA442459 - JP 2018138019-A/14385: Polycomb-Associated Non-Coding RNAs.
MA449298 - JP 2018138019-A/21224: Polycomb-Associated Non-Coding RNAs.
MA594534 - JP 2018138019-A/166460: Polycomb-Associated Non-Coding RNAs.
MA594535 - JP 2018138019-A/166461: Polycomb-Associated Non-Coding RNAs.
MP584522 - Sequence 21 from Patent WO2020081767.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P09341 (Reactome details) participates in the following event(s):

R-HSA-373813 Receptor CXCR2 binds ligands CXCL1 to 7
R-HSA-6798749 Exocytosis of specific granule lumen proteins
R-HSA-6798745 Exocytosis of tertiary granule lumen proteins
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-6783783 Interleukin-10 signaling
R-HSA-6798695 Neutrophil degranulation
R-HSA-418594 G alpha (i) signalling events
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-449147 Signaling by Interleukins
R-HSA-168249 Innate Immune System
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000395761.1, ENST00000395761.2, ENST00000395761.3, GRO, GRO1, GROA, GROA_HUMAN, MGSA, NR_046035, P09341, Q9UCR7, SCYB1, uc318xrr.1, uc318xrr.2
UCSC ID: ENST00000395761.4_4
RefSeq Accession: NM_001511.4
Protein: P09341 (aka GROA_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.