ID:CPSF3_HUMAN DESCRIPTION: RecName: Full=Cleavage and polyadenylation specificity factor subunit 3; EC=3.1.27.-; AltName: Full=Cleavage and polyadenylation specificity factor 73 kDa subunit; Short=CPSF 73 kDa subunit; AltName: Full=mRNA 3'-end-processing endonuclease CPSF-73; FUNCTION: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. COFACTOR: Binds 2 zinc ions per subunit. SUBUNIT: Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF2, CSTF2 and SYMPK. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of pre-mRNAs. Interacts with WDR33. SUBCELLULAR LOCATION: Nucleus. PTM: Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UKF6
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC030988 - Homo sapiens cleavage and polyadenylation specific factor 3, 73kDa, mRNA (cDNA clone IMAGE:4806442), partial cds. AK298918 - Homo sapiens cDNA FLJ57562 complete cds, highly similar to Cleavage and polyadenylation specificityfactor 73 kDa subunit. BC011654 - Homo sapiens cleavage and polyadenylation specific factor 3, 73kDa, mRNA (cDNA clone MGC:12899 IMAGE:4304135), complete cds. AK223487 - Homo sapiens mRNA for cleavage and polyadenylation specific factor 3, 73kDa variant, clone: FCC119G02. BC020211 - Homo sapiens cleavage and polyadenylation specific factor 3, 73kDa, mRNA (cDNA clone MGC:31848 IMAGE:4890593), complete cds. AF171877 - Homo sapiens cleavage and polyadenylation specificity factor 73 kDa subunit mRNA, complete cds. EU176402 - Synthetic construct Homo sapiens clone IMAGE:100006608; FLH264002.01X; RZPDo839B10245D cleavage and polyadenylation specific factor 3, 73kDa (CPSF3) gene, encodes complete protein. BC014106 - Homo sapiens, Similar to cleavage and polyadenylation specificity factor 3, clone IMAGE:4130680, mRNA. BC043432 - Homo sapiens cleavage and polyadenylation specific factor 3, 73kDa, mRNA (cDNA clone IMAGE:5295012), partial cds. AK025440 - Homo sapiens cDNA: FLJ21787 fis, clone HEP00329, highly similar to AF171877 Homo sapiens cleavage and polyadenylation specificity factor 73 kDa subunit mRNA. AF017269 - Homo sapiens 73 kDA subunit of cleavage and polyadenylation specificity factor mRNA, partial cds. JD025256 - Sequence 6280 from Patent EP1572962. EF036508 - Homo sapiens cleavage and polyadenylation specific factor 3 mRNA, partial cds. JD138866 - Sequence 119890 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_cpsfPathway - Polyadenylation of mRNA
Reactome (by CSHL, EBI, and GO)
Protein Q9UKF6 (Reactome details) participates in the following event(s):
R-HSA-72231 Cleavage and Polyadenylation R-HSA-77589 Recognition of AAUAAA sequence by CPSF R-HSA-72185 mRNA polyadenylation R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-77590 Recruitment of CstF to the CPSF Bound Pre-mRNA R-HSA-77592 Cleavage of Intronless Pre-mRNA at 3'-end R-HSA-77593 Cleavage and polyadenylation of Intronless Pre-mRNA R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-156661 Formation of Exon Junction Complex R-HSA-77591 Binding of Cleavage factors and Poly(A)Polymerase to the CstF:CPSF:Pre-mRNA Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-77595 Processing of Intronless Pre-mRNAs R-HSA-72187 mRNA 3'-end processing R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-72172 mRNA Splicing R-HSA-75067 Processing of Capped Intronless Pre-mRNA R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-8953854 Metabolism of RNA R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
