Human Gene CNOT8 (ENST00000285896.11_4) from GENCODE V47lift37

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Description: CCR4-NOT transcription complex subunit 8, transcript variant 1 (from RefSeq NM_004779.6)
Gencode Transcript: ENST00000285896.11_4
Gencode Gene: ENSG00000155508.14_11
Transcript (Including UTRs)
Position: hg19 chr5:154,238,187-154,256,352 Size: 18,166 Total Exon Count: 7 Strand: +
Coding Region
Position: hg19 chr5:154,242,839-154,254,999 Size: 12,161 Coding Exon Count: 6

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr5:154,238,187-154,256,352)			mRNA (may differ from genome)		Protein (292 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	Reactome	UniProtKB
Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: CNOT8_HUMAN DESCRIPTION: RecName: Full=CCR4-NOT transcription complex subunit 8; AltName: Full=CAF1-like protein; Short=CALIFp; AltName: Full=CAF2; AltName: Full=CCR4-associated factor 8; FUNCTION: Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex. SUBUNIT: Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds BTG2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. SIMILARITY: Belongs to the CAF1 family.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C006780 bisphenol A D002794 Choline D019327 Copper Sulfate D003993 Dibutyl Phthalate D004147 Dioxins D005492 Folic Acid D008715 Methionine D011794 Quercetin D012643 Selenium C018021 cobaltous chloride more ... click here to view the complete list

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 35.47 RPKM in Adipose - Subcutaneous Total median expression: 952.07 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-82.70	214	-0.386	Picture	PostScript	Text
3' UTR	-345.80	1353	-0.256	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR006941 - RNase_CAF1
IPR012337 - RNaseH-like_dom

Pfam Domains:
PF04857 - CAF1 family ribonuclease

SCOP Domains:
53098 - Ribonuclease H-like

ModBase Predicted Comparative 3D Structure on Q9UFF9


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	Genome Browser
Gene Details	Gene Details				Gene Details
Gene Sorter	Gene Sorter				Gene Sorter
	RGD	Ensembl			SGD
					Protein Sequence
					Alignment

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0000175 3'-5'-exoribonuclease activity GO:0003676 nucleic acid binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0003723 RNA binding GO:0004518 nuclease activity GO:0004527 exonuclease activity GO:0004535 poly(A)-specific ribonuclease activity GO:0005515 protein binding GO:0016787 hydrolase activity GO:0046872 metal ion binding Biological Process: GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006417 regulation of translation GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0008284 positive regulation of cell proliferation GO:0008285 negative regulation of cell proliferation GO:0031047 gene silencing by RNA GO:0035195 gene silencing by miRNA GO:0043928 exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0061014 positive regulation of mRNA catabolic process GO:0090305 nucleic acid phosphodiester bond hydrolysis GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic Cellular Component: GO:0000932 P-body GO:0005622 intracellular GO:0005634 nucleus GO:0005737 cytoplasm GO:0005829 cytosol GO:0030014 CCR4-NOT complex GO:0030015 CCR4-NOT core complex

Descriptions from all associated GenBank mRNAs


	AK316269 - Homo sapiens cDNA, FLJ79168 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. AK316221 - Homo sapiens cDNA, FLJ79120 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. AK314317 - Homo sapiens cDNA, FLJ95075, highly similar to Homo sapiens CCR4-NOT transcription complex, subunit 8 (CNOT8), mRNA. AK316055 - Homo sapiens cDNA, FLJ78954 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. BC093025 - Homo sapiens cDNA clone IMAGE:30344920, containing frame-shift errors. AK299589 - Homo sapiens cDNA FLJ52273 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. AK025358 - Homo sapiens cDNA: FLJ21705 fis, clone COL09940. AK315864 - Homo sapiens cDNA, FLJ79513 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. AK309359 - Homo sapiens cDNA, FLJ99400. AL122045 - Homo sapiens mRNA; cDNA DKFZp434M1515 (from clone DKFZp434M1515). JD396015 - Sequence 377039 from Patent EP1572962. AF053318 - Homo sapiens CCR4-associated factor 1 (POP2) mRNA, complete cds. AK303775 - Homo sapiens cDNA FLJ52044 complete cds, moderately similar to CCR4-NOT transcription complex subunit 8. AK297381 - Homo sapiens cDNA FLJ50580 complete cds, highly similar to CCR4-NOT transcription complex subunit 8. BC017366 - Homo sapiens CCR4-NOT transcription complex, subunit 8, mRNA (cDNA clone MGC:1191 IMAGE:3506054), complete cds. JD021164 - Sequence 2188 from Patent EP1572962. JD033198 - Sequence 14222 from Patent EP1572962. AF180476 - Homo sapiens CALIFp (CALIF) mRNA, complete cds. JD103163 - Sequence 84187 from Patent EP1572962. AF087844 - Homo sapiens CAF2 mRNA, complete cds. JD368119 - Sequence 349143 from Patent EP1572962. JD528662 - Sequence 509686 from Patent EP1572962. AB463473 - Synthetic construct DNA, clone: pF1KB8321, Homo sapiens CNOT8 gene for CCR4-NOT transcription complex, subunit 8, without stop codon, in Flexi system. AM393646 - Synthetic construct Homo sapiens clone IMAGE:100001801 for hypothetical protein (CNOT8 gene). BT006857 - Homo sapiens CCR4-NOT transcription complex, subunit 8 mRNA, complete cds. KJ892749 - Synthetic construct Homo sapiens clone ccsbBroadEn_02143 CNOT8 gene, encodes complete protein. BX647124 - Homo sapiens mRNA; cDNA DKFZp686M1099 (from clone DKFZp686M1099). JD082827 - Sequence 63851 from Patent EP1572962. JD138603 - Sequence 119627 from Patent EP1572962. JD119650 - Sequence 100674 from Patent EP1572962. JD080347 - Sequence 61371 from Patent EP1572962. JD436592 - Sequence 417616 from Patent EP1572962. JD419218 - Sequence 400242 from Patent EP1572962. JD059163 - Sequence 40187 from Patent EP1572962. JD419849 - Sequence 400873 from Patent EP1572962. JD308003 - Sequence 289027 from Patent EP1572962. JD500202 - Sequence 481226 from Patent EP1572962. JD288133 - Sequence 269157 from Patent EP1572962. JD290255 - Sequence 271279 from Patent EP1572962. JD113212 - Sequence 94236 from Patent EP1572962. JD058261 - Sequence 39285 from Patent EP1572962. JD176167 - Sequence 157191 from Patent EP1572962. JD318695 - Sequence 299719 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein Q9UFF9 (Reactome details) participates in the following event(s): R-HSA-6798044 BTG2 binds CCR4-NOT complex R-HSA-429955 CCR4-NOT complex deadenylates mRNA R-HSA-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain R-HSA-6791312 TP53 Regulates Transcription of Cell Cycle Genes R-HSA-429947 Deadenylation of mRNA R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-429914 Deadenylation-dependent mRNA decay R-HSA-212436 Generic Transcription Pathway R-HSA-8953854 Metabolism of RNA R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)

Other Names for This Gene


	Alternate Gene Symbols: B0AZS3, B2RAR8, B7Z8R1, CALIF, CNOT8_HUMAN, D3DQI8, ENST00000285896.1, ENST00000285896.10, ENST00000285896.2, ENST00000285896.3, ENST00000285896.4, ENST00000285896.5, ENST00000285896.6, ENST00000285896.7, ENST00000285896.8, ENST00000285896.9, NM_004779, O95709, POP2, Q7Z521, Q9H6Y1, Q9UFF9, uc317kio.1, uc317kio.2 UCSC ID: ENST00000285896.11_4 RefSeq Accession: NM_001301073.2 Protein: Q9UFF9 (aka CNOT8_HUMAN or CNT8_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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