Human Gene CNKSR1 (ENST00000361530.11_8) from GENCODE V47lift37

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Description: connector enhancer of kinase suppressor of Ras 1, transcript variant 1 (from RefSeq NM_006314.3)
Gencode Transcript: ENST00000361530.11_8
Gencode Gene: ENSG00000142675.18_12
Transcript (Including UTRs)
Position: hg19 chr1:26,503,982-26,516,375 Size: 12,394 Total Exon Count: 21 Strand: +
Coding Region
Position: hg19 chr1:26,504,039-26,516,039 Size: 12,001 Coding Exon Count: 21

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr1:26,503,982-26,516,375)			mRNA (may differ from genome)		Protein (713 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	Reactome	UniProtKB
Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: CNKR1_HUMAN DESCRIPTION: RecName: Full=Connector enhancer of kinase suppressor of ras 1; Short=Connector enhancer of KSR 1; AltName: Full=CNK homolog protein 1; Short=CNK1; Short=hCNK1; AltName: Full=Connector enhancer of KSR-like; FUNCTION: May function as an adapter protein or regulator of Ras signaling pathways. SUBUNIT: Interacts with RHO and RALGDS. INTERACTION: P61586:RHOA; NbExp=4; IntAct=EBI-741671, EBI-446668; SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). PTM: Phosphorylated on tyrosine. SIMILARITY: Belongs to the CNKSR family. SIMILARITY: Contains 1 CRIC domain. SIMILARITY: Contains 1 PDZ (DHR) domain. SIMILARITY: Contains 1 PH domain. SIMILARITY: Contains 1 SAM (sterile alpha motif) domain.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D013739 Testosterone D013749 Tetrachlorodibenzodioxin C547126 AZM551248 D017638 Asbestos, Crocidolite D001564 Benzo(a)pyrene D016572 Cyclosporine D003907 Dexamethasone D004147 Dioxins D007545 Isoproterenol D014212 Tretinoin more ... click here to view the complete list

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 18.18 RPKM in Muscle - Skeletal Total median expression: 233.38 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-13.60	57	-0.239	Picture	PostScript	Text
3' UTR	-83.00	336	-0.247	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR017874 - CRIC_domain
IPR019555 - CRIC_domain_Chordata
IPR001478 - PDZ
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology
IPR001660 - SAM
IPR013761 - SAM/pointed
IPR021129 - SAM_type1

Pfam Domains:
PF00169 - PH domain
PF00536 - SAM domain (Sterile alpha motif)
PF10534 - Connector enhancer of kinase suppressor of ras

SCOP Domains:
47769 - SAM/Pointed domain
50156 - PDZ domain-like
50729 - PH domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1WWV - NMR MuPIT

ModBase Predicted Comparative 3D Structure on Q969H4


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD	Ensembl

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0005515 protein binding GO:0030674 protein binding, bridging Biological Process: GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway GO:0007265 Ras protein signal transduction GO:0007266 Rho protein signal transduction Cellular Component: GO:0005737 cytoplasm GO:0005886 plasma membrane GO:0005911 cell-cell junction GO:0005938 cell cortex GO:0016020 membrane

Descriptions from all associated GenBank mRNAs


	AK296814 - Homo sapiens cDNA FLJ60656 complete cds, highly similar to Connector enhancer of kinase suppressor ofras 1. AB209335 - Homo sapiens mRNA for Connector enhancer of kinase suppressor of ras 1 variant protein. AK222904 - Homo sapiens mRNA for connector enhancer of kinase suppressor of Ras 1 variant, clone: HRC04910. BC011604 - Homo sapiens connector enhancer of kinase suppressor of Ras 1, mRNA (cDNA clone MGC:1887 IMAGE:3139227), complete cds. BC012797 - Homo sapiens connector enhancer of kinase suppressor of Ras 1, mRNA (cDNA clone MGC:1888 IMAGE:3505293), complete cds. AK302056 - Homo sapiens cDNA FLJ59272 complete cds, highly similar to Connector enhancer of kinase suppressor of ras 1. AK292646 - Homo sapiens cDNA FLJ78632 complete cds, highly similar to Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), mRNA. AK001900 - Homo sapiens cDNA FLJ11038 fis, clone PLACE1004358, highly similar to Homo sapiens connector enhancer of KSR-like protein CNK1 mRNA. AF100153 - Homo sapiens connector enhancer of KSR-like protein CNK1 mRNA, complete cds. AK222817 - Homo sapiens mRNA for connector enhancer of kinase suppressor of Ras 1 variant, clone: HEP06524. JD373100 - Sequence 354124 from Patent EP1572962. AB590388 - Synthetic construct DNA, clone: pFN21AB5712, Homo sapiens CNKSR1 gene for connector enhancer of kinase suppressor of Ras 1, without stop codon, in Flexi system. DQ892241 - Synthetic construct clone IMAGE:100004871; FLH184429.01X; RZPDo839F11144D connector enhancer of kinase suppressor of Ras 1 (CNKSR1) gene, encodes complete protein. DQ895441 - Synthetic construct Homo sapiens clone IMAGE:100009901; FLH184425.01L; RZPDo839F11143D connector enhancer of kinase suppressor of Ras 1 (CNKSR1) gene, encodes complete protein. CU679980 - Synthetic construct Homo sapiens gateway clone IMAGE:100020411 5' read CNKSR1 mRNA. KJ902081 - Synthetic construct Homo sapiens clone ccsbBroadEn_11475 CNKSR1 gene, encodes complete protein. BT006900 - Homo sapiens connector enhancer of KSR-like (Drosophila kinase suppressor of ras) mRNA, complete cds. JD105122 - Sequence 86146 from Patent EP1572962. JD228203 - Sequence 209227 from Patent EP1572962. JD214105 - Sequence 195129 from Patent EP1572962. JD202342 - Sequence 183366 from Patent EP1572962. JD322303 - Sequence 303327 from Patent EP1572962. JD189152 - Sequence 170176 from Patent EP1572962. JD399557 - Sequence 380581 from Patent EP1572962. JD132400 - Sequence 113424 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein Q969H4 (Reactome details) participates in the following event(s): R-HSA-5672972 MAP2Ks and MAPKs bind to the activated RAF complex R-HSA-6802912 High kinase activity BRAF mutants bind MAP2Ks and MAPKs R-HSA-6802914 RAS:GTP:moderate kinase activity p-RAF complexes bind MAP2Ks and MAPKs R-HSA-6802925 Mutant RAS:p-RAF complexes bind MAP2Ks and MAPKs R-HSA-6802934 p-BRAF and RAF fusion dimers bind MAP2Ks and MAPKs R-HSA-6802942 RAS:GTP:p-RAF complexes paradoxically bind MAP2Ks and MAPKs R-HSA-5672980 Dissociation of RAS:RAF complex R-HSA-6802932 Dissociation of BRAF/RAF fusion complex R-HSA-6803227 Dissociation of high activity BRAF complexes R-HSA-6803230 Dissociation of moderate activity BRAF complexes R-HSA-6803233 Dissociation of oncogenic RAS:RAF complex R-HSA-6803234 Dissociation of paradoxically activated RAS:BRAF complexes R-HSA-5672978 RAF phosphorylates MAP2K dimer R-HSA-5672973 MAP2Ks phosphorylate MAPKs R-HSA-6802918 Activated MAP2Ks phosphorylate MAPKs downstream of inactive BRAF mutants R-HSA-6802943 RAS:GTP:inactive p-RAF complexes phosphorylate MAP2Ks R-HSA-6802919 RAS:GTP:moderate kinase activity p-RAF complexes phosphorylate MAP2Ks R-HSA-6802921 Activated MAP2Ks phosphorylate MAPKs downstream of moderate kinase activity BRAF mutants R-HSA-6802911 High kinase activity BRAF complexes phosphorylate MAP2Ks R-HSA-6802910 Activated MAP2Ks phosphorylate MAPKs downstream of high kinase activity BRAF mutants R-HSA-6802926 Mutant RAS:p-RAF complexes phosphorylate MAP2Ks R-HSA-6802922 Activated MAP2Ks phosphorylate MAPKs downstream of oncogenic RAS R-HSA-6802933 p-BRAF and RAF fusion dimers phosphorylate MAP2Ks R-HSA-6802935 MAPKs are phosphorylated downstream of BRAF and RAF fusion dimers R-HSA-5674135 MAP2K and MAPK activation R-HSA-6802948 Signaling by high-kinase activity BRAF mutants R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants R-HSA-6802949 Signaling by RAS mutants R-HSA-6802952 Signaling by BRAF and RAF fusions R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-5673001 RAF/MAP kinase cascade R-HSA-6802957 Oncogenic MAPK signaling R-HSA-5684996 MAPK1/MAPK3 signaling R-HSA-5663202 Diseases of signal transduction R-HSA-5683057 MAPK family signaling cascades R-HSA-1643685 Disease R-HSA-162582 Signal Transduction

Other Names for This Gene


	Alternate Gene Symbols: B1AMW9, CNK1, CNKR1_HUMAN, ENST00000361530.1, ENST00000361530.10, ENST00000361530.2, ENST00000361530.3, ENST00000361530.4, ENST00000361530.5, ENST00000361530.6, ENST00000361530.7, ENST00000361530.8, ENST00000361530.9, NM_006314, O95381, Q969H4, uc318cdk.1, uc318cdk.2 UCSC ID: ENST00000361530.11_8 RefSeq Accession: NM_006314.3 Protein: Q969H4 (aka CNKR1_HUMAN or CNR1_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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