ID:CAF1A_HUMAN DESCRIPTION: RecName: Full=Chromatin assembly factor 1 subunit A; Short=CAF-1 subunit A; AltName: Full=Chromatin assembly factor I p150 subunit; Short=CAF-I 150 kDa subunit; Short=CAF-I p150; Short=hp150; FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. SUBUNIT: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. INTERACTION: P45973:CBX5; NbExp=3; IntAct=EBI-1020839, EBI-78219; Q99684:GFI1; NbExp=4; IntAct=EBI-1020839, EBI-949368; P16333:NCK1; NbExp=2; IntAct=EBI-1020839, EBI-389883; P12004:PCNA; NbExp=2; IntAct=EBI-1020839, EBI-358311; SUBCELLULAR LOCATION: Nucleus. Note=DNA replication foci. DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases. DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the CHAF1A family. SEQUENCE CAUTION: Sequence=AAA76736.1; Type=Erroneous initiation; Sequence=AAF04291.1; Type=Erroneous initiation; Sequence=AAH52620.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH52620.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence starting in position 426;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13111
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC067093 - Homo sapiens chromatin assembly factor 1, subunit A (p150), mRNA (cDNA clone MGC:71229 IMAGE:6339027), complete cds. BC052620 - Homo sapiens chromatin assembly factor 1, subunit A (p150), mRNA (cDNA clone IMAGE:6579095), partial cds. BC041606 - Homo sapiens, Similar to chromatin assembly factor 1, subunit A (p150), clone IMAGE:5483594, mRNA. AB527824 - Synthetic construct DNA, clone: pF1KB8521, Homo sapiens CHAF1A gene for chromatin assembly factor 1, subunit A, without stop codon, in Flexi system. U20979 - Human chromatin assembly factor-I p150 subunit mRNA, complete cds. BC005813 - Homo sapiens chromatin assembly factor 1, subunit A (p150), mRNA (cDNA clone IMAGE:3842956), partial cds. BC011560 - Homo sapiens chromatin assembly factor 1, subunit A (p150), mRNA (cDNA clone IMAGE:4653034), with apparent retained intron. LF341705 - JP 2014500723-A/149208: Polycomb-Associated Non-Coding RNAs. JD429563 - Sequence 410587 from Patent EP1572962. JD169916 - Sequence 150940 from Patent EP1572962. JD490533 - Sequence 471557 from Patent EP1572962. JD314963 - Sequence 295987 from Patent EP1572962. JD530865 - Sequence 511889 from Patent EP1572962. MA577282 - JP 2018138019-A/149208: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_btg2Pathway - BTG family proteins and cell cycle regulation h_vdrPathway - Control of Gene Expression by Vitamin D Receptor
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
