ID:CENPU_HUMAN DESCRIPTION: RecName: Full=Centromere protein U; Short=CENP-U; AltName: Full=Interphase centromere complex protein 24; AltName: Full=KSHV latent nuclear antigen-interacting protein 1; AltName: Full=MLF1-interacting protein; AltName: Full=Polo-box-interacting protein 1; AltName: Full=centromere protein of 50 kDa; Short=CENP-50; FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. SUBUNIT: Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma- associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. TISSUE SPECIFICITY: Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines. PTM: Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q71F23
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF516710 - Homo sapiens MLF1 interacting protein (MLF1IP) mRNA, complete cds. BC131556 - Homo sapiens MLF1 interacting protein, mRNA (cDNA clone MGC:148077 IMAGE:40108113), complete cds. BC107744 - Homo sapiens MLF1 interacting protein, mRNA (cDNA clone IMAGE:6618811), partial cds. BC031520 - Homo sapiens MLF1 interacting protein, mRNA (cDNA clone IMAGE:4345397), complete cds. AK027121 - Homo sapiens cDNA: FLJ23468 fis, clone HSI11603. BC067802 - Homo sapiens MLF1 interacting protein, mRNA (cDNA clone IMAGE:5298168). BC141854 - Homo sapiens MLF1 interacting protein, mRNA (cDNA clone MGC:167037 IMAGE:8860370), complete cds. AF469667 - Homo sapiens KSHV latent nuclear antigen interacting protein 1 (KLIP1) mRNA, complete cds. JD305666 - Sequence 286690 from Patent EP1572962. AK292297 - Homo sapiens cDNA FLJ77371 complete cds, highly similar to Homo sapiens MLF1 interacting protein (MLF1IP), mRNA. KJ903200 - Synthetic construct Homo sapiens clone ccsbBroadEn_12594 MLF1IP gene, encodes complete protein. KJ894713 - Synthetic construct Homo sapiens clone ccsbBroadEn_04107 MLF1IP gene, encodes complete protein. CR457376 - Homo sapiens full open reading frame cDNA clone RZPDo834H0110D for gene KLIP1, KSHV latent nuclear antigen interacting protein 1; complete cds, incl. stopcodon. DQ907911 - Homo sapiens MLF1 interacting protein isoform 2 (MLF1IP) mRNA, complete cds, alternatively spliced. DQ907910 - Homo sapiens MLF1 interacting protein isoform 1 (MLF1IP) mRNA, complete cds, alternatively spliced. JD295086 - Sequence 276110 from Patent EP1572962. JD243576 - Sequence 224600 from Patent EP1572962. JD248179 - Sequence 229203 from Patent EP1572962. JD098361 - Sequence 79385 from Patent EP1572962. JD552464 - Sequence 533488 from Patent EP1572962. JD113155 - Sequence 94179 from Patent EP1572962. JD302209 - Sequence 283233 from Patent EP1572962. JD042807 - Sequence 23831 from Patent EP1572962. JD436064 - Sequence 417088 from Patent EP1572962. JD564777 - Sequence 545801 from Patent EP1572962. JD092673 - Sequence 73697 from Patent EP1572962. JD188346 - Sequence 169370 from Patent EP1572962. JD309885 - Sequence 290909 from Patent EP1572962. JD083073 - Sequence 64097 from Patent EP1572962. JD522220 - Sequence 503244 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q71F23 (Reactome details) participates in the following event(s):
R-HSA-606349 Mis18 complex binds the centromere R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-606326 HJURP:CENPA complex localizes to the centromere R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-774815 Nucleosome assembly R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-73886 Chromosome Maintenance R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-1640170 Cell Cycle R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
