ID:MPIP2_HUMAN DESCRIPTION: RecName: Full=M-phase inducer phosphatase 2; EC=3.1.3.48; AltName: Full=Dual specificity phosphatase Cdc25B; FUNCTION: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. ENZYME REGULATION: Stimulated by B-type cyclins. SUBUNIT: Interacts with MAPK14 and 14-3-3 proteins. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. PTM: Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. SIMILARITY: Belongs to the MPI phosphatase family. SIMILARITY: Contains 1 rhodanese domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc25b/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P30305
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK295573 - Homo sapiens cDNA FLJ53333 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK299192 - Homo sapiens cDNA FLJ56140 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK299028 - Homo sapiens cDNA FLJ55479 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK092713 - Homo sapiens cDNA FLJ35394 fis, clone SKNSH2002768, highly similar to M-PHASE INDUCER PHOSPHATASE 2 (EC 3.1.3.48). FR695900 - Homo sapiens mRNA for isoform CDC25B6 (CDC25B gene). FR695901 - Homo sapiens mRNA for isoform CDC25B7 (CDC25B gene). BX647988 - Homo sapiens mRNA; cDNA DKFZp686N1752 (from clone DKFZp686N1752). BC051711 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:44614 IMAGE:5301205), complete cds. JD065989 - Sequence 47013 from Patent EP1572962. S78187 - CDC25Hu2=cdc25+ homolog [human, mRNA, 3118 nt]. JD389850 - Sequence 370874 from Patent EP1572962. JD194242 - Sequence 175266 from Patent EP1572962. BC009953 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:14269 IMAGE:4130804), complete cds. Z68092 - H.sapiens mRNA for tyrosine phosphatase. DL492376 - Novel nucleic acids. JD456935 - Sequence 437959 from Patent EP1572962. JD456936 - Sequence 437960 from Patent EP1572962. BC006395 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:12797 IMAGE:4135465), complete cds. DL490864 - Novel nucleic acids. M81934 - Human cdc25B mRNA, complete cds. JD405826 - Sequence 386850 from Patent EP1572962. JD405870 - Sequence 386894 from Patent EP1572962. JD125348 - Sequence 106372 from Patent EP1572962. JD391573 - Sequence 372597 from Patent EP1572962. JD132313 - Sequence 113337 from Patent EP1572962. KJ890879 - Synthetic construct Homo sapiens clone ccsbBroadEn_00273 CDC25B gene, encodes complete protein. BX640836 - Homo sapiens mRNA; cDNA DKFZp686G14213 (from clone DKFZp686G14213). AK124284 - Homo sapiens cDNA FLJ42290 fis, clone TLIVE2007132. JD133729 - Sequence 114753 from Patent EP1572962. JD395818 - Sequence 376842 from Patent EP1572962. JD182891 - Sequence 163915 from Patent EP1572962. JD223839 - Sequence 204863 from Patent EP1572962. JD232565 - Sequence 213589 from Patent EP1572962. JD399819 - Sequence 380843 from Patent EP1572962. JD561720 - Sequence 542744 from Patent EP1572962. JD234260 - Sequence 215284 from Patent EP1572962. JD149248 - Sequence 130272 from Patent EP1572962. JD510182 - Sequence 491206 from Patent EP1572962. JD510419 - Sequence 491443 from Patent EP1572962. JD465732 - Sequence 446756 from Patent EP1572962. JD424793 - Sequence 405817 from Patent EP1572962. JD387464 - Sequence 368488 from Patent EP1572962. JD130488 - Sequence 111512 from Patent EP1572962. JD449626 - Sequence 430650 from Patent EP1572962. JD354421 - Sequence 335445 from Patent EP1572962. JD357467 - Sequence 338491 from Patent EP1572962. JD419842 - Sequence 400866 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_cdc25Pathway - cdc25 and chk1 Regulatory Pathway in response to DNA damage h_rbPathway - RB Tumor Suppressor/Checkpoint Signaling in response to DNA damage h_g2Pathway - Cell Cycle: G2/M Checkpoint h_ptc1Pathway - Sonic Hedgehog (SHH) Receptor Ptc1 Regulates cell cycle h_srcRPTPPathway - Activation of Src by Protein-tyrosine phosphatase alpha
Reactome (by CSHL, EBI, and GO)
Protein P30305 (Reactome details) participates in the following event(s):
R-HSA-170120 Translocation of Cdc25B to the cytoplasm R-HSA-8863007 p25-bound CDK5 phosphorylates CDC25B R-HSA-170159 Translocation of Cdc25 to the nucleus R-HSA-170161 Dephosphorylation of cytoplasmic Cyclin B1/B2:phospho-Cdc2 (Thr 14, Tyr 15) complexes by CDC25B R-HSA-170153 Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases R-HSA-174110 Cdc25A mediated dephosphorylation of Cyclin A:phospho-Cdk2 R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-8862803 Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models R-HSA-69275 G2/M Transition R-HSA-8863678 Neurodegenerative Diseases R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry R-HSA-453274 Mitotic G2-G2/M phases R-HSA-1643685 Disease R-HSA-69242 S Phase R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
