Human Gene CD86 (ENST00000330540.7_10) from GENCODE V47lift37
  Description: CD86 molecule, transcript variant 1 (from RefSeq NM_175862.5)
Gencode Transcript: ENST00000330540.7_10
Gencode Gene: ENSG00000114013.16_18
Transcript (Including UTRs)
   Position: hg19 chr3:121,774,209-121,839,983 Size: 65,775 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr3:121,774,337-121,838,381 Size: 64,045 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:121,774,209-121,839,983)mRNA (may differ from genome)Protein (329 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CD86_HUMAN
DESCRIPTION: RecName: Full=T-lymphocyte activation antigen CD86; AltName: Full=Activation B7-2 antigen; AltName: Full=B70; AltName: Full=BU63; AltName: Full=CTLA-4 counter-receptor B7.2; AltName: Full=FUN-1; AltName: CD_antigen=CD86; Flags: Precursor;
FUNCTION: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
SUBUNIT: Homodimer. Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.
INTERACTION: P16410:CTLA4; NbExp=2; IntAct=EBI-1030956, EBI-1030991;
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed by activated B-lymphocytes and monocytes.
PTM: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
SIMILARITY: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
WEB RESOURCE: Name=Wikipedia; Note=CD86 entry; URL="http://en.wikipedia.org/wiki/CD86";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CD86
Diseases sorted by gene-association score: gallbladder squamous cell carcinoma (14), myocarditis (13), plague (12), lymphohistiocytoid mesothelioma (11), skin carcinoma in situ (10), epididymis cancer (10), epididymis adenocarcinoma (10), acute myocarditis (9), penicilliosis (7), bartholin's gland benign neoplasm (7), paracoccidioidomycosis (7), acute cervicitis (6), autoimmune disease of central nervous system (6), vulval paget's disease (6), vulva adenocarcinoma (5), malignant epithelial mesothelioma (5), breast squamous cell carcinoma (5), systemic lupus erythematosus (5), myositis fibrosa (5), diabetes mellitus, insulin-dependent, 13 (5), diabetes mellitus, insulin-dependent, 6 (4), cataract 14, multiple types (4), exanthema subitum (4), rheumatoid arthritis (3), common variable immunodeficiency (3), multiple sclerosis, disease progression, modifier of (1), differentiated thyroid carcinoma (1), leukemia, acute myeloid (0)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.59 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 85.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.40128-0.237 Picture PostScript Text
3' UTR -459.001602-0.287 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR013106 - Ig_V-set
IPR003596 - Ig_V-set_subgr

Pfam Domains:
PF07686 - Immunoglobulin V-set domain
PF13895 - Immunoglobulin domain

SCOP Domains:
48726 - Immunoglobulin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1I85 - X-ray MuPIT 1NCN - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P42081
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001618 virus receptor activity
GO:0005515 protein binding
GO:0015026 coreceptor activity
GO:0038023 signaling receptor activity
GO:0046934 phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Biological Process:
GO:0002250 adaptive immune response
GO:0002376 immune system process
GO:0006955 immune response
GO:0008284 positive regulation of cell proliferation
GO:0016032 viral process
GO:0019221 cytokine-mediated signaling pathway
GO:0031295 T cell costimulation
GO:0043017 positive regulation of lymphotoxin A biosynthetic process
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045404 positive regulation of interleukin-4 biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
GO:0045893 positive regulation of transcription, DNA-templated
GO:0046718 viral entry into host cell
GO:0046854 phosphatidylinositol phosphorylation
GO:0051897 positive regulation of protein kinase B signaling

Cellular Component:
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LQ740457 - Sequence 11 from Patent WO2018083189.
AK301237 - Homo sapiens cDNA FLJ58631 complete cds, highly similar to T-lymphocyte activation antigen CD86 precursor.
AK316203 - Homo sapiens cDNA, FLJ79102 complete cds, highly similar to T-lymphocyte activation antigen CD86 precursor.
AK294663 - Homo sapiens cDNA FLJ50144 complete cds, highly similar to T-lymphocyte activation antigen CD86 precursor.
BC040261 - Homo sapiens CD86 molecule, mRNA (cDNA clone MGC:34413 IMAGE:5173789), complete cds.
L25259 - Human CTLA4 counter-receptor (B7-2) mRNA, complete cds.
JD203853 - Sequence 184877 from Patent EP1572962.
AK291497 - Homo sapiens cDNA FLJ76858 complete cds, highly similar to Homo sapiens CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) (CD86), transcript variant 1, mRNA.
JD297431 - Sequence 278455 from Patent EP1572962.
AK313103 - Homo sapiens cDNA, FLJ93588, highly similar to Homo sapiens CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) (CD86), mRNA.
U04343 - Human CD86 antigen mRNA, complete cds.
FW340041 - Screening.
DQ893787 - Synthetic construct Homo sapiens clone IMAGE:100008247; FLH164390.01L; RZPDo839F10161D CD86 molecule (CD86) gene, encodes complete protein.
DQ890617 - Synthetic construct Homo sapiens clone IMAGE:100003247; FLH263530.01X; RZPDo839F09162D CD86 molecule (CD86) gene, encodes complete protein.
KJ896564 - Synthetic construct Homo sapiens clone ccsbBroadEn_05958 CD86 gene, encodes complete protein.
CR541844 - Homo sapiens full open reading frame cDNA clone RZPDo834D1232D for gene CD86, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); complete cds, without stopcodon.
KU284848 - Homo sapiens CD86 antigen (CD86) mRNA, complete cds.
JD298162 - Sequence 279186 from Patent EP1572962.
JD566912 - Sequence 547936 from Patent EP1572962.
JD102757 - Sequence 83781 from Patent EP1572962.
JD324820 - Sequence 305844 from Patent EP1572962.
JD097923 - Sequence 78947 from Patent EP1572962.
JD298605 - Sequence 279629 from Patent EP1572962.
JD538558 - Sequence 519582 from Patent EP1572962.
JD348331 - Sequence 329355 from Patent EP1572962.
JD294321 - Sequence 275345 from Patent EP1572962.
JD261372 - Sequence 242396 from Patent EP1572962.
JD444605 - Sequence 425629 from Patent EP1572962.
JD437420 - Sequence 418444 from Patent EP1572962.
JD339424 - Sequence 320448 from Patent EP1572962.
JD151163 - Sequence 132187 from Patent EP1572962.
JD058668 - Sequence 39692 from Patent EP1572962.
JD566414 - Sequence 547438 from Patent EP1572962.
JD067578 - Sequence 48602 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_ctla4Pathway - The Co-Stimulatory Signal During T-cell Activation
h_th1th2Pathway - Th1/Th2 Differentiation

Reactome (by CSHL, EBI, and GO)

Protein P42081 (Reactome details) participates in the following event(s):

R-HSA-388808 CD28 homodimer binds B7-2 monomer
R-HSA-388809 CTLA-4 binds B7-1/B7-2
R-HSA-388831 Phosphorylation of CD28
R-HSA-388814 Grb2 binds CD28
R-HSA-388832 PI3K binds CD28
R-HSA-389381 Gads binds CD28
R-HSA-388833 Phosphorylation of CTLA-4
R-HSA-389352 Translocation of Vav1 to CD28
R-HSA-388829 SHP2 phosphatase binds CTLA-4
R-HSA-389158 CD28 bound PI3K phosphorylates PIP2 to PIP3
R-HSA-389354 Activation of Vav1
R-HSA-2400009 PI3K inhibitors block PI3K catalytic activity
R-HSA-2316434 PI3K phosphorylates PIP2 to PIP3
R-HSA-389348 Activation of Rac1 by pVav1
R-HSA-389350 Activation of Cdc42 by pVav1
R-HSA-389356 CD28 co-stimulation
R-HSA-389513 CTLA4 inhibitory signaling
R-HSA-6783783 Interleukin-10 signaling
R-HSA-388841 Costimulation by the CD28 family
R-HSA-389359 CD28 dependent Vav1 pathway
R-HSA-389357 CD28 dependent PI3K/Akt signaling
R-HSA-449147 Signaling by Interleukins
R-HSA-1280218 Adaptive Immune System
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System
R-HSA-2219530 Constitutive Signaling by Aberrant PI3K in Cancer
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-2219528 PI3K/AKT Signaling in Cancer
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-199418 Negative regulation of the PI3K/AKT network
R-HSA-5663202 Diseases of signal transduction
R-HSA-162582 Signal Transduction
R-HSA-1643685 Disease

-  Other Names for This Gene
  Alternate Gene Symbols: A0N0P0, B7Z2F3, B7Z702, CD28LG2, CD86_HUMAN, E7ETN5, E9PC27, ENST00000330540.1, ENST00000330540.2, ENST00000330540.3, ENST00000330540.4, ENST00000330540.5, ENST00000330540.6, NM_175862, P42081, Q13655, Q6FHB1, Q6GTS4, Q7M4L5, uc317szp.1, uc317szp.2
UCSC ID: ENST00000330540.7_10
RefSeq Accession: NM_175862.5
Protein: P42081 (aka CD86_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.