ID:BRF2_HUMAN DESCRIPTION: RecName: Full=Transcription factor IIIB 50 kDa subunit; Short=TFIIIB50; Short=hTFIIIB50; AltName: Full=B-related factor 2; Short=BRF-2; AltName: Full=hBRFU; FUNCTION: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SUBUNIT: Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4. SUBCELLULAR LOCATION: Nucleus. INDUCTION: Down-regulated by epigallocatechin gallate (EGCG) treatment. SIMILARITY: Belongs to the TFIIB family. SIMILARITY: Contains 1 TFIIB-type zinc finger. SEQUENCE CAUTION: Sequence=AAG35486.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9HAW0
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
JD019532 - Sequence 556 from Patent EP1572962. JD027720 - Sequence 8744 from Patent EP1572962. JD021211 - Sequence 2235 from Patent EP1572962. JD031609 - Sequence 12633 from Patent EP1572962. JD021198 - Sequence 2222 from Patent EP1572962. AF130058 - Homo sapiens clone FLB5619 PRO1470 mRNA, complete cds. BC010648 - Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, mRNA (cDNA clone MGC:9916 IMAGE:3871326), complete cds. AF206673 - Homo sapiens TFIIIB50 mRNA, complete cds. AK001914 - Homo sapiens cDNA FLJ11052 fis, clone PLACE1004645. JD033972 - Sequence 14996 from Patent EP1572962. JD448036 - Sequence 429060 from Patent EP1572962. JD243962 - Sequence 224986 from Patent EP1572962. JD226354 - Sequence 207378 from Patent EP1572962. JD178252 - Sequence 159276 from Patent EP1572962. JD255036 - Sequence 236060 from Patent EP1572962. JD060226 - Sequence 41250 from Patent EP1572962. JD162178 - Sequence 143202 from Patent EP1572962. JD450442 - Sequence 431466 from Patent EP1572962. JD043902 - Sequence 24926 from Patent EP1572962. JD053631 - Sequence 34655 from Patent EP1572962. AF298153 - Homo sapiens RNA polymerase III transcription initiation factor BRFU mRNA, complete cds. AK294337 - Homo sapiens cDNA FLJ51823 complete cds, highly similar to Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mRNA. AK315420 - Homo sapiens cDNA, FLJ96474, Homo sapiens BRF2, subunit of RNA polymerase III transcriptioninitiation factor, BRF1-like (BRF2), mRNA. KJ894179 - Synthetic construct Homo sapiens clone ccsbBroadEn_03573 BRF2 gene, encodes complete protein. EU446514 - Synthetic construct Homo sapiens clone IMAGE:100069872; IMAGE:100011723; FLH258608.01L BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2) gene, encodes complete protein. AB463449 - Synthetic construct DNA, clone: pF1KB7707, Homo sapiens BRF2 gene for BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, without stop codon, in Flexi system. CU679783 - Synthetic construct Homo sapiens gateway clone IMAGE:100017226 5' read BRF2 mRNA. JD234433 - Sequence 215457 from Patent EP1572962. JD547205 - Sequence 528229 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9HAW0 (Reactome details) participates in the following event(s):
R-HSA-83793 Binding of TFIIIB to SNAPc:Oct-1:Staf:Type 3 Promoter Complex R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation R-HSA-76046 RNA Polymerase III Transcription Initiation R-HSA-74158 RNA Polymerase III Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
