Human Gene AUP1 (ENST00000377526.4_7) from GENCODE V47lift37
  Description: AUP1 lipid droplet regulating VLDL assembly factor, transcript variant 2 (from RefSeq NM_181575.5)
Gencode Transcript: ENST00000377526.4_7
Gencode Gene: ENSG00000115307.17_9
Transcript (Including UTRs)
   Position: hg19 chr2:74,753,779-74,756,833 Size: 3,055 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr2:74,753,927-74,756,756 Size: 2,830 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:74,753,779-74,756,833)mRNA (may differ from genome)Protein (410 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AUP1_HUMAN
DESCRIPTION: RecName: Full=Ancient ubiquitous protein 1;
FUNCTION: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
SUBUNIT: Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM.
INTERACTION: O94817:ATG12; NbExp=1; IntAct=EBI-1058701, EBI-746742; Q13418:ILK; NbExp=1; IntAct=EBI-1058701, EBI-747644; Q99608:NDN; NbExp=1; IntAct=EBI-1058701, EBI-718177; P60604:UBE2G2; NbExp=1; IntAct=EBI-1058701, EBI-1051028;
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side.
TISSUE SPECIFICITY: Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.
SIMILARITY: Belongs to the AUP1 family.
SIMILARITY: Contains 1 CUE domain.
SEQUENCE CAUTION: Sequence=AAH33646.2; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 144.02 RPKM in Spleen
Total median expression: 4004.04 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.1077-0.391 Picture PostScript Text
3' UTR -40.70148-0.275 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003892 - CUE

Pfam Domains:
PF02845 - CUE domain

SCOP Domains:
69593 - Glycerol-3-phosphate (1)-acyltransferase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2EKF - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9Y679
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0043130 ubiquitin binding
GO:0097027 ubiquitin-protein transferase activator activity

Biological Process:
GO:0030433 ER-associated ubiquitin-dependent protein catabolic process
GO:0030970 retrograde protein transport, ER to cytosol
GO:0050790 regulation of catalytic activity

Cellular Component:
GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030176 integral component of endoplasmic reticulum membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF207216 - JP 2014500723-A/14719: Polycomb-Associated Non-Coding RNAs.
BC001658 - Homo sapiens ancient ubiquitous protein 1, mRNA (cDNA clone MGC:1122 IMAGE:3050204), complete cds.
AK055566 - Homo sapiens cDNA FLJ31004 fis, clone HLUNG2000063, highly similar to Homo sapiens ancient ubiquitous 46 kDa protein AUP1 mRNA.
AF100746 - Homo sapiens AUP1 homolog mRNA, complete cds.
CR749661 - Homo sapiens mRNA; cDNA DKFZp686P12272 (from clone DKFZp686P12272).
BC033646 - Homo sapiens ancient ubiquitous protein 1, mRNA (cDNA clone IMAGE:5578910), partial cds.
AF100753 - Homo sapiens ancient ubiquitous 46 kDa protein AUP1 mRNA, complete cds.
AF100754 - Homo sapiens ancient ubiquitous protein AUP1 isoform mRNA, complete cds.
AF165515 - Homo sapiens ancient ubiquitous protein 1 precursor (AUP1) mRNA, complete cds.
AK023983 - Homo sapiens cDNA FLJ13921 fis, clone Y79AA1000469, highly similar to Mus musculus ancient ubiquitous 46 kDa protein AUP1 precursor (Aup1) mRNA.
AK293121 - Homo sapiens cDNA FLJ76490 complete cds, highly similar to Homo sapiens ancient ubiquitous protein 1 (AUP1), transcript variant 2, mRNA.
AK304886 - Homo sapiens cDNA FLJ57204 complete cds, highly similar to Homo sapiens ancient ubiquitous protein 1 (AUP1), transcript variant 2, mRNA.
JD207371 - Sequence 188395 from Patent EP1572962.
JD412613 - Sequence 393637 from Patent EP1572962.
JD095565 - Sequence 76589 from Patent EP1572962.
JD120781 - Sequence 101805 from Patent EP1572962.
JD204855 - Sequence 185879 from Patent EP1572962.
JD103984 - Sequence 85008 from Patent EP1572962.
LF363225 - JP 2014500723-A/170728: Polycomb-Associated Non-Coding RNAs.
JD420654 - Sequence 401678 from Patent EP1572962.
JD479415 - Sequence 460439 from Patent EP1572962.
JD098055 - Sequence 79079 from Patent EP1572962.
EU831790 - Synthetic construct Homo sapiens clone HAIB:100066819; DKFZo008D1021 ancient ubiquitous protein 1 protein (AUP1) gene, encodes complete protein.
EU831868 - Synthetic construct Homo sapiens clone HAIB:100066897; DKFZo004D1022 ancient ubiquitous protein 1 protein (AUP1) gene, encodes complete protein.
KJ896480 - Synthetic construct Homo sapiens clone ccsbBroadEn_05874 AUP1 gene, encodes complete protein.
AB527869 - Synthetic construct DNA, clone: pF1KB0421, Homo sapiens AUP1 gene for ancient ubiquitous protein 1, without stop codon, in Flexi system.
JD414273 - Sequence 395297 from Patent EP1572962.
LF363224 - JP 2014500723-A/170727: Polycomb-Associated Non-Coding RNAs.
JD221423 - Sequence 202447 from Patent EP1572962.
AK310159 - Homo sapiens cDNA, FLJ17201.
AK308231 - Homo sapiens cDNA, FLJ98179.
KJ901299 - Synthetic construct Homo sapiens clone ccsbBroadEn_10693 AUP1 gene, encodes complete protein.
LF363223 - JP 2014500723-A/170726: Polycomb-Associated Non-Coding RNAs.
LF363222 - JP 2014500723-A/170725: Polycomb-Associated Non-Coding RNAs.
CU689682 - Synthetic construct Homo sapiens gateway clone IMAGE:100023281 5' read AUP1 mRNA.
LF363221 - JP 2014500723-A/170724: Polycomb-Associated Non-Coding RNAs.
CU674818 - Synthetic construct Homo sapiens gateway clone IMAGE:100023090 5' read AUP1 mRNA.
LF363220 - JP 2014500723-A/170723: Polycomb-Associated Non-Coding RNAs.
AK301398 - Homo sapiens cDNA FLJ58836 complete cds, highly similar to Ancient ubiquitous protein 1 precursor.
LF207214 - JP 2014500723-A/14717: Polycomb-Associated Non-Coding RNAs.
JD464743 - Sequence 445767 from Patent EP1572962.
JD395718 - Sequence 376742 from Patent EP1572962.
MA598802 - JP 2018138019-A/170728: Polycomb-Associated Non-Coding RNAs.
MA598801 - JP 2018138019-A/170727: Polycomb-Associated Non-Coding RNAs.
MA598800 - JP 2018138019-A/170726: Polycomb-Associated Non-Coding RNAs.
MA598799 - JP 2018138019-A/170725: Polycomb-Associated Non-Coding RNAs.
MA598798 - JP 2018138019-A/170724: Polycomb-Associated Non-Coding RNAs.
MA598797 - JP 2018138019-A/170723: Polycomb-Associated Non-Coding RNAs.
MA442793 - JP 2018138019-A/14719: Polycomb-Associated Non-Coding RNAs.
MA442791 - JP 2018138019-A/14717: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: AUP1 , AUP1_HUMAN, C0H5W8, ENST00000377526.1, ENST00000377526.2, ENST00000377526.3, NM_181575, Q9H866, Q9UNQ6, Q9Y679, Q9Y685, uc318nrj.1, uc318nrj.2
UCSC ID: ENST00000377526.4_7
RefSeq Accession: NM_181575.5
Protein: Q9Y679 (aka AUP1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.