Human Gene AMOT (ENST00000371959.9_12) from GENCODE V47lift37
  Description: angiomotin, transcript variant 4 (from RefSeq NM_001386999.1)
Gencode Transcript: ENST00000371959.9_12
Gencode Gene: ENSG00000126016.17_15
Transcript (Including UTRs)
   Position: hg19 chrX:112,018,105-112,084,059 Size: 65,955 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chrX:112,021,795-112,066,354 Size: 44,560 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:112,018,105-112,084,059)mRNA (may differ from genome)Protein (1084 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MalacardsMGIPubMedReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AMOT_HUMAN
DESCRIPTION: RecName: Full=Angiomotin;
FUNCTION: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
SUBUNIT: Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.
INTERACTION: Q68EM7:ARHGAP17; NbExp=4; IntAct=EBI-3891843, EBI-1642807; P35240:NF2; NbExp=2; IntAct=EBI-3903812, EBI-1014472;
SUBCELLULAR LOCATION: Cell junction, tight junction. Note=Localized on the cell surface. May act as a transmembrane protein.
TISSUE SPECIFICITY: Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
DOMAIN: The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
DOMAIN: The angiostatin binding domain (871-1005) allows the binding to angiostatin.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
PTM: Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.
MISCELLANEOUS: 'Motus' means 'motility' in Latin.
SIMILARITY: Belongs to the angiomotin family.
SEQUENCE CAUTION: Sequence=AAH94712.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: AMOT
Diseases sorted by gene-association score: papilloma (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.62 RPKM in Brain - Caudate (basal ganglia)
Total median expression: 176.41 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -234.50668-0.351 Picture PostScript Text
3' UTR -1027.703690-0.279 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009114 - Angiomotin
IPR024646 - Angiomotin_C

Pfam Domains:
PF12240 - Angiomotin C terminal

ModBase Predicted Comparative 3D Structure on Q4VCS5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0038023 signaling receptor activity
GO:0042802 identical protein binding
GO:0043532 angiostatin binding

Biological Process:
GO:0001570 vasculogenesis
GO:0001701 in utero embryonic development
GO:0001702 gastrulation with mouth forming second
GO:0003365 establishment of cell polarity involved in ameboidal cell migration
GO:0006935 chemotaxis
GO:0007043 cell-cell junction assembly
GO:0016525 negative regulation of angiogenesis
GO:0030036 actin cytoskeleton organization
GO:0030334 regulation of cell migration
GO:0034260 negative regulation of GTPase activity
GO:0034613 cellular protein localization
GO:0035329 hippo signaling
GO:0040019 positive regulation of embryonic development
GO:0042074 cell migration involved in gastrulation
GO:0043116 negative regulation of vascular permeability
GO:0043534 blood vessel endothelial cell migration
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0045793 positive regulation of cell size
GO:0048514 blood vessel morphogenesis
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051496 positive regulation of stress fiber assembly

Cellular Component:
GO:0001725 stress fiber
GO:0001726 ruffle
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005884 actin filament
GO:0005923 bicellular tight junction
GO:0009897 external side of plasma membrane
GO:0009986 cell surface
GO:0016021 integral component of membrane
GO:0030027 lamellipodium
GO:0030054 cell junction
GO:0030139 endocytic vesicle
GO:0031410 cytoplasmic vesicle
GO:0008180 COP9 signalosome


-  Descriptions from all associated GenBank mRNAs
  AF286598 - Homo sapiens angiomotin mRNA, complete cds.
AK301573 - Homo sapiens cDNA FLJ60926 complete cds, highly similar to Angiomotin.
BC094712 - Homo sapiens angiomotin, mRNA (cDNA clone IMAGE:30718677), partial cds.
BC039408 - Homo sapiens angiomotin, mRNA (cDNA clone IMAGE:5301410).
JD327774 - Sequence 308798 from Patent EP1572962.
BC050581 - Homo sapiens, clone IMAGE:6043566, mRNA.
AB028994 - Homo sapiens KIAA1071 mRNA for KIAA1071 protein.
AK024344 - Homo sapiens cDNA FLJ14282 fis, clone PLACE1005802.
JD548915 - Sequence 529939 from Patent EP1572962.
JD296255 - Sequence 277279 from Patent EP1572962.
JD122894 - Sequence 103918 from Patent EP1572962.
JD355313 - Sequence 336337 from Patent EP1572962.
JD313908 - Sequence 294932 from Patent EP1572962.
JD548552 - Sequence 529576 from Patent EP1572962.
JD211950 - Sequence 192974 from Patent EP1572962.
JD278382 - Sequence 259406 from Patent EP1572962.
JD314548 - Sequence 295572 from Patent EP1572962.
JD196911 - Sequence 177935 from Patent EP1572962.
JD268408 - Sequence 249432 from Patent EP1572962.
JD308581 - Sequence 289605 from Patent EP1572962.
JD510606 - Sequence 491630 from Patent EP1572962.
JD068542 - Sequence 49566 from Patent EP1572962.
JD438087 - Sequence 419111 from Patent EP1572962.
JD360721 - Sequence 341745 from Patent EP1572962.
JD037569 - Sequence 18593 from Patent EP1572962.
JD321567 - Sequence 302591 from Patent EP1572962.
JD099439 - Sequence 80463 from Patent EP1572962.
JD539977 - Sequence 521001 from Patent EP1572962.
JD298609 - Sequence 279633 from Patent EP1572962.
JD233154 - Sequence 214178 from Patent EP1572962.
JD412588 - Sequence 393612 from Patent EP1572962.
JD297012 - Sequence 278036 from Patent EP1572962.
JD501586 - Sequence 482610 from Patent EP1572962.
JD479256 - Sequence 460280 from Patent EP1572962.
JD349441 - Sequence 330465 from Patent EP1572962.
JD109453 - Sequence 90477 from Patent EP1572962.
JD280564 - Sequence 261588 from Patent EP1572962.
JD549066 - Sequence 530090 from Patent EP1572962.
JD089247 - Sequence 70271 from Patent EP1572962.
JD098928 - Sequence 79952 from Patent EP1572962.
JD260513 - Sequence 241537 from Patent EP1572962.
JD562552 - Sequence 543576 from Patent EP1572962.
JD378946 - Sequence 359970 from Patent EP1572962.
JD492854 - Sequence 473878 from Patent EP1572962.
JD267825 - Sequence 248849 from Patent EP1572962.
JD322012 - Sequence 303036 from Patent EP1572962.
JD069083 - Sequence 50107 from Patent EP1572962.
JD422588 - Sequence 403612 from Patent EP1572962.
JD076928 - Sequence 57952 from Patent EP1572962.
JD473356 - Sequence 454380 from Patent EP1572962.
JD436683 - Sequence 417707 from Patent EP1572962.
JD468683 - Sequence 449707 from Patent EP1572962.
JD485342 - Sequence 466366 from Patent EP1572962.
JD345308 - Sequence 326332 from Patent EP1572962.
JD067525 - Sequence 48549 from Patent EP1572962.
JD258232 - Sequence 239256 from Patent EP1572962.
JD168646 - Sequence 149670 from Patent EP1572962.
JD081047 - Sequence 62071 from Patent EP1572962.
JD183739 - Sequence 164763 from Patent EP1572962.
JD123888 - Sequence 104912 from Patent EP1572962.
BC130294 - Homo sapiens angiomotin, mRNA (cDNA clone MGC:163166 IMAGE:40146325), complete cds.
DQ571267 - Homo sapiens piRNA piR-31379, complete sequence.
JD566050 - Sequence 547074 from Patent EP1572962.
JD553614 - Sequence 534638 from Patent EP1572962.
JD562737 - Sequence 543761 from Patent EP1572962.
JD300663 - Sequence 281687 from Patent EP1572962.
KJ903901 - Synthetic construct Homo sapiens clone ccsbBroadEn_13295 AMOT gene, encodes complete protein.
AY987378 - Homo sapiens angiomotin p130 isoform (AMOT) mRNA, complete cds, alternatively spliced.
HQ258584 - Synthetic construct Homo sapiens clone IMAGE:100073116 Unknown protein gene, encodes complete protein.
AB384557 - Synthetic construct DNA, clone: pF1KA1071, Homo sapiens AMOT gene for angiomotin, complete cds, without stop codon, in Flexi system.
JD186820 - Sequence 167844 from Patent EP1572962.
JD351166 - Sequence 332190 from Patent EP1572962.
JD410919 - Sequence 391943 from Patent EP1572962.
JD422516 - Sequence 403540 from Patent EP1572962.
JD320472 - Sequence 301496 from Patent EP1572962.
JD411116 - Sequence 392140 from Patent EP1572962.
JD472941 - Sequence 453965 from Patent EP1572962.
JD203618 - Sequence 184642 from Patent EP1572962.
JD115378 - Sequence 96402 from Patent EP1572962.
JD204989 - Sequence 186013 from Patent EP1572962.
JD210699 - Sequence 191723 from Patent EP1572962.
JD152628 - Sequence 133652 from Patent EP1572962.
JD394399 - Sequence 375423 from Patent EP1572962.
JD406330 - Sequence 387354 from Patent EP1572962.
JD390360 - Sequence 371384 from Patent EP1572962.
JD153792 - Sequence 134816 from Patent EP1572962.
JD254472 - Sequence 235496 from Patent EP1572962.
JD259953 - Sequence 240977 from Patent EP1572962.
JD164243 - Sequence 145267 from Patent EP1572962.
JD402543 - Sequence 383567 from Patent EP1572962.
JD555219 - Sequence 536243 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q4VCS5 (Reactome details) participates in the following event(s):

R-HSA-2028724 AMOT proteins bind YAP1
R-HSA-2028735 AMOT proteins bind WWTR1 (TAZ)
R-HSA-2028269 Signaling by Hippo
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: AMOT_HUMAN, ENST00000371959.1, ENST00000371959.2, ENST00000371959.3, ENST00000371959.4, ENST00000371959.5, ENST00000371959.6, ENST00000371959.7, ENST00000371959.8, KIAA1071, NM_001386999, Q4VCS5, Q504X5, Q9HD27, Q9UPT1, uc318jns.1, uc318jns.2
UCSC ID: ENST00000371959.9_12
RefSeq Accession: NM_001113490.2
Protein: Q4VCS5 (aka AMOT_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.