ID:AKAP9_HUMAN DESCRIPTION: RecName: Full=A-kinase anchor protein 9; Short=AKAP-9; AltName: Full=A-kinase anchor protein 350 kDa; Short=AKAP 350; Short=hgAKAP 350; AltName: Full=A-kinase anchor protein 450 kDa; Short=AKAP 450; AltName: Full=AKAP 120-like protein; AltName: Full=Centrosome- and Golgi-localized PKN-associated protein; Short=CG-NAP; AltName: Full=Protein hyperion; AltName: Full=Protein kinase A-anchoring protein 9; Short=PRKA9; AltName: Full=Protein yotiao; FUNCTION: Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. SUBUNIT: Interacts with the regulatory region of protein kinase N (PKN), protein phosphatase 2A (PP2A), protein phosphatase 1 (PP1) and the immature non-phosphorylated form of PKC epsilon. Interacts with CIP4 and FNBP1. Interacts with chloride intracellular channel proteins CLIC1, CLIC4 and CLIC5. CSNK1D binding promotes its centrosomal subcellular location. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Golgi apparatus. Note=Cytoplasmic in parietal cells. TISSUE SPECIFICITY: Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas. DOMAIN: RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. SEQUENCE CAUTION: Sequence=AAB86384.1; Type=Frameshift; Positions=1637; Sequence=AAC60380.1; Type=Erroneous gene model prediction; WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/AKAP9ID42999ch7q21.html";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF10495 - Pericentrin-AKAP-450 domain of centrosomal targeting protein
ModBase Predicted Comparative 3D Structure on Q99996
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AJ131693 - Homo sapiens mRNA for AKAP450 protein. AF026245 - Homo sapiens yotiao mRNA, complete cds. BC015533 - Homo sapiens A kinase (PRKA) anchor protein (yotiao) 9, mRNA (cDNA clone IMAGE:3914749), complete cds. AB019691 - Homo sapiens mRNA for Centrosome- and Golgi-localized PKN-associated protein (CG-NAP), complete cds. AY803272 - Homo sapiens AKAP9-BRAF fusion protein mRNA, complete cds. KJ902063 - Synthetic construct Homo sapiens clone ccsbBroadEn_11457 AKAP9 gene, encodes complete protein. BC172915 - Synthetic construct Homo sapiens clone IMAGE:9094491 A-kinase anchor protein 9 isoform 3 (AKAP9) gene, partial cds. CU677341 - Synthetic construct Homo sapiens gateway clone IMAGE:100022663 5' read AKAP9 mRNA. AF247727 - Homo sapiens AKAP350C mRNA, complete cds, alternatively spliced. JD200882 - Sequence 181906 from Patent EP1572962. JD407779 - Sequence 388803 from Patent EP1572962. JD095143 - Sequence 76167 from Patent EP1572962. JD501388 - Sequence 482412 from Patent EP1572962. AF083037 - Homo sapiens A-kinase anchoring protein AKAP350 mRNA, partial cds. AY528715 - Homo sapiens antigen MU-RMS-40.16A mRNA, partial cds. BC062448 - Homo sapiens A kinase (PRKA) anchor protein (yotiao) 9, mRNA (cDNA clone IMAGE:4249102). AL117418 - Homo sapiens mRNA; cDNA DKFZp564G2263 (from clone DKFZp564G2263). BC172916 - Synthetic construct Homo sapiens clone IMAGE:9094492 A-kinase anchor protein 9 isoform 3 (AKAP9) gene, partial cds. AF091711 - Homo sapiens splice variant AKAP350 mRNA, partial cds. AB018346 - Homo sapiens mRNA for KIAA0803 protein, partial cds. AK074869 - Homo sapiens cDNA FLJ90388 fis, clone NT2RP2005425, highly similar to A-kinase anchor protein 9. AK000270 - Homo sapiens cDNA FLJ20263 fis, clone COLF7804, highly similar to AJ131693 Homo sapiens mRNA for AKAP450 protein. AK057546 - Homo sapiens cDNA FLJ32984 fis, clone THYMU1000017, highly similar to Homo sapiens splice variant AKAP350 mRNA. AK026444 - Homo sapiens cDNA: FLJ22791 fis, clone KAIA2181, highly similar to HSA131693 Homo sapiens mRNA for AKAP450 protein. BC027455 - Homo sapiens A kinase (PRKA) anchor protein (yotiao) 9, mRNA (cDNA clone IMAGE:4510491), with apparent retained intron. AK025160 - Homo sapiens cDNA: FLJ21507 fis, clone COL05722, highly similar to AF091711 Homo sapiens splice variant AKAP350 mRNA. BC040932 - Homo sapiens, clone IMAGE:4540494, mRNA, partial cds. JD315551 - Sequence 296575 from Patent EP1572962. JD297278 - Sequence 278302 from Patent EP1572962. JD184509 - Sequence 165533 from Patent EP1572962. JD548831 - Sequence 529855 from Patent EP1572962. JD086197 - Sequence 67221 from Patent EP1572962. JD304283 - Sequence 285307 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_akapCentrosomePathway - Protein Kinase A at the Centrosome
Reactome (by CSHL, EBI, and GO)
Protein Q99996 (Reactome details) participates in the following event(s):
R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-432172 Activation of NMDA receptor R-HSA-432162 Unblocking of NMDA receptor R-HSA-5577050 AKAP9:KCNQ1 tetramer:KCNE dimer transports K+ from cytosol to extracellular region R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-6802927 BRAF and RAF fusion mutant dimers are phosphorylated R-HSA-6802934 p-BRAF and RAF fusion dimers bind MAP2Ks and MAPKs R-HSA-6802932 Dissociation of BRAF/RAF fusion complex R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-442760 Activation of RasGRF R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-6802933 p-BRAF and RAF fusion dimers phosphorylate MAP2Ks R-HSA-6802935 MAPKs are phosphorylated downstream of BRAF and RAF fusion dimers R-HSA-432164 Ca2+ influx into the post-synaptic cell R-HSA-445367 CaMKII enters cytoplasm R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-442732 Ras activation R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-5672965 RAS GEFs promote RAS nucleotide exchange R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-438066 Unblocking of NMDA receptor, glutamate binding and activation R-HSA-5576890 Phase 3 - rapid repolarisation R-HSA-5576893 Phase 2 - plateau phase R-HSA-5617833 Cilium Assembly R-HSA-6802952 Signaling by BRAF and RAF fusions R-HSA-68877 Mitotic Prometaphase R-HSA-69275 G2/M Transition R-HSA-442982 Ras activation upon Ca2+ influx through NMDA receptor R-HSA-442755 Activation of NMDA receptor and postsynaptic events R-HSA-5576891 Cardiac conduction R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-6802957 Oncogenic MAPK signaling R-HSA-68886 M Phase R-HSA-453274 Mitotic G2-G2/M phases R-HSA-442742 CREB phosphorylation through the activation of Ras R-HSA-442729 CREB phosphorylation through the activation of CaMKII R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission R-HSA-397014 Muscle contraction R-HSA-5663202 Diseases of signal transduction R-HSA-69278 Cell Cycle (Mitotic) R-HSA-438064 Post NMDA receptor activation events R-HSA-112315 Transmission across Chemical Synapses R-HSA-1643685 Disease R-HSA-1640170 Cell Cycle R-HSA-5673001 RAF/MAP kinase cascade R-HSA-112316 Neuronal System R-HSA-5684996 MAPK1/MAPK3 signaling R-HSA-5683057 MAPK family signaling cascades R-HSA-162582 Signal Transduction
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Common Gene Haplotype Alleles 
