Human Gene ADH5 (ENST00000296412.14_7) from GENCODE V47lift37
  Description: alcohol dehydrogenase 5 (class III), chi polypeptide (from RefSeq NM_000671.4)
Gencode Transcript: ENST00000296412.14_7
Gencode Gene: ENSG00000197894.12_14
Transcript (Including UTRs)
   Position: hg19 chr4:99,992,129-100,009,939 Size: 17,811 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr4:99,993,568-100,009,851 Size: 16,284 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:99,992,129-100,009,939)mRNA (may differ from genome)Protein (374 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: Q6IRT1_HUMAN
DESCRIPTION: SubName: Full=Alcohol dehydrogenase 5 (Class III), chi polypeptide; SubName: Full=Alcohol dehydrogenase 5 (Class III), chi polypeptide, isoform CRA_a; SubName: Full=cDNA, FLJ92689, highly similar to Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide(ADH5), mRNA;
COFACTOR: Zinc (By similarity).

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ADH5
Diseases sorted by gene-association score: methanol poisoning (18), pharyngitis (7), alcohol dependence (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 104.47 RPKM in Esophagus - Muscularis
Total median expression: 2023.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -22.1088-0.251 Picture PostScript Text
3' UTR -335.901439-0.233 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR014183 - ADH_3
IPR013149 - ADH_C
IPR013154 - ADH_GroES-like
IPR002085 - ADH_SF_Zn-type
IPR002328 - ADH_Zn_CS
IPR011032 - GroES-like
IPR016040 - NAD(P)-bd_dom

Pfam Domains:
PF00107 - Zinc-binding dehydrogenase
PF08240 - Alcohol dehydrogenase GroES-like domain

SCOP Domains:
50129 - GroES-like
51735 - NAD(P)-binding Rossmann-fold domains
53335 - S-adenosyl-L-methionine-dependent methyltransferases

ModBase Predicted Comparative 3D Structure on Q6IRT1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004022 alcohol dehydrogenase (NAD) activity
GO:0008270 zinc ion binding
GO:0016491 oxidoreductase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity

Biological Process:
GO:0001523 retinoid metabolic process
GO:0003016 respiratory system process
GO:0006069 ethanol oxidation
GO:0018119 peptidyl-cysteine S-nitrosylation
GO:0032496 response to lipopolysaccharide
GO:0045777 positive regulation of blood pressure
GO:0046294 formaldehyde catabolic process
GO:0051409 response to nitrosative stress
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005739 mitochondrion


-  Descriptions from all associated GenBank mRNAs
  M30471 - Human class III alcohol dehydrogenase (ADH5) chi subunit mRNA, complete cds.
M29872 - Human alcohol dehydrogenase class III (ADH5) mRNA, complete cds.
BC014665 - Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide, mRNA (cDNA clone MGC:23126 IMAGE:4853366), complete cds.
GQ472236 - Homo sapiens epididymis secretory sperm binding protein Li 60p (HEL-S-60p) mRNA, complete cds.
BC070491 - Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide, mRNA (cDNA clone MGC:87925 IMAGE:6164452), complete cds.
AK312367 - Homo sapiens cDNA, FLJ92689, highly similar to Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide(ADH5), mRNA.
KJ890634 - Synthetic construct Homo sapiens clone ccsbBroadEn_00028 ADH5 gene, encodes complete protein.
AB462942 - Synthetic construct DNA, clone: pF1KB5582, Homo sapiens ADH5 gene for alcohol dehydrogenase 5 (class III), chi polypeptide, without stop codon, in Flexi system.
AK129570 - Homo sapiens cDNA FLJ26059 fis, clone PRS03891, highly similar to Alcohol dehydrogenase class III chi chain (EC 1.1.1.1).
AK226177 - Homo sapiens mRNA for class III alcohol dehydrogenase 5 chi subunit variant, clone: ef06333.
JD062961 - Sequence 43985 from Patent EP1572962.
JD504311 - Sequence 485335 from Patent EP1572962.
JD377351 - Sequence 358375 from Patent EP1572962.
JD092215 - Sequence 73239 from Patent EP1572962.
JD092214 - Sequence 73238 from Patent EP1572962.
JD433446 - Sequence 414470 from Patent EP1572962.
JD433445 - Sequence 414469 from Patent EP1572962.
JD080679 - Sequence 61703 from Patent EP1572962.
JD234997 - Sequence 216021 from Patent EP1572962.
JD114243 - Sequence 95267 from Patent EP1572962.
JD094206 - Sequence 75230 from Patent EP1572962.
JD214266 - Sequence 195290 from Patent EP1572962.
JD155564 - Sequence 136588 from Patent EP1572962.
JD051993 - Sequence 33017 from Patent EP1572962.
JD051992 - Sequence 33016 from Patent EP1572962.
JD304589 - Sequence 285613 from Patent EP1572962.
JD337210 - Sequence 318234 from Patent EP1572962.
JD406276 - Sequence 387300 from Patent EP1572962.
JD183080 - Sequence 164104 from Patent EP1572962.
AK124994 - Homo sapiens cDNA FLJ43004 fis, clone BRTHA2013540.
CR536491 - Homo sapiens full open reading frame cDNA clone RZPDo834D0420D for gene ADH5, alcohol dehydrogenase 5 (class III), chi polypeptide; complete cds, incl. stopcodon.
BT019832 - Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide mRNA, complete cds.
BT019833 - Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide mRNA, complete cds.
CR541689 - Homo sapiens full open reading frame cDNA clone RZPDo834D0928D for gene ADH5, alcohol dehydrogenase 5 (class III), chi polypeptide; complete cds, without stopcodon.
JD022236 - Sequence 3260 from Patent EP1572962.
JD028091 - Sequence 9115 from Patent EP1572962.
JD227762 - Sequence 208786 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-1801 - formaldehyde oxidation II (glutathione-dependent)
PWY-7798 - protein S-nitrosylation and denitrosylation

-  Other Names for This Gene
  Alternate Gene Symbols: A0A024RDI2, ADH5 , ENST00000296412.1, ENST00000296412.10, ENST00000296412.11, ENST00000296412.12, ENST00000296412.13, ENST00000296412.2, ENST00000296412.3, ENST00000296412.4, ENST00000296412.5, ENST00000296412.6, ENST00000296412.7, ENST00000296412.8, ENST00000296412.9, hCG_41483 , HEL-S-60p , NM_000671, Q6IRT1, Q6IRT1_HUMAN, uc317ltg.1, uc317ltg.2
UCSC ID: ENST00000296412.14_7
RefSeq Accession: NM_000671.4
Protein: Q6IRT1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.