| Treatment | Description | Label | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| None | No special treatment or protocol applies | No treatment or prot | ||||||||
| androgen | 12 hrs with 1 nM Methyltrienolone (R1881) (Crawford) | Methyltrienolone | ||||||||
| estrogen | 45 min with 100 nM Estradiol (Crawford) | Estradiol 100 nM | ||||||||
| serum_starved_media | Grown with normal serum levels (10%), then switched to media containing low level of FBS (0.05%) for 72 hours | serum-starved media | ||||||||
| serum_stimulated_media | Grown with normal serum levels (10%), then switched to media that contains no FBS for 36 hours, then switched back to normal serum levels (10% FBS) for 3 hours. | serum-stimulated media | ||||||||
| vehicle | Charcoal stripped hormone-free FBS for 72 hours (Crawford) | Vehicle | ||||||||