Gene interactions and pathways from curated databases and text-mining
J Cell Biol 1993, PMID: 8245132

Sequence and tissue distribution of the integrin alpha 9 subunit, a novel partner of beta 1 that is widely distributed in epithelia and muscle.

Palmer, E L; Rüegg, C; Ferrando, R; Pytela, R; Sheppard, D

The integrin family of adhesion receptors consists of several heterodimeric glycoproteins, each composed of one alpha and one beta subunit. A novel integrin alpha subunit partial cDNA isolated from TGF-beta stimulated guinea pig airway epithelial cells has previously been reported (Erle, D.J., D. Sheppard, J. Bruess, C. Rüegg, and R. Pytela. 1991. Am. J. Respir. Cell Mol. Biol. 5:170-177). We have now determined cDNA and amino acid sequence for the human homolog of this subunit, named alpha 9, from a human lung cDNA library, a human small intestine cDNA library, and cDNA from the cell lines U937, HL-60 and Tera-2. This sequence is predicted to encode a 1006-amino acid mature protein that shares 39% identity with the previously identified integrin subunit alpha 4. By Northern blot analysis, alpha 9 mRNA was detected in the human carcinoma cell lines Tera-2 and Caco-2. Anti-peptide antibodies against the predicted COOH-terminal sequence of alpha 9 immunoprecipitated a heterodimer (140 kD/115 kD nonreduced; 150 kD/130 kD reduced) from Tera-2 lysates. Immunodepletion of beta 1-containing integrins with Tera-2 lysates removed alpha 9 immunoreactivity, suggesting that beta 1 is the principal beta subunit partner for alpha 9 in these cells. alpha 9 was detected by immunohistochemistry in airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes.

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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: ITGB1 — ITGA9 (physical association, affinity chromatography technology)
  • Gene Ontology Complexes integrin complex: integrin complex complex (ITGAX-ITGB1-ITGB2-ITGB5-ITGB7-ITGB6-ITGA9-ITGA2B-ITGA2-ITGA3-ITGA4-ITGAV-ITGA10-ITGA8-ITGA6-ITGB8-ITGA1-ITGA11-ITGAD-ITGAE)
  • IRef Hprd Interaction: ITGB1 — ITGA9 (in vitro)
  • IRef Hprd Interaction: ITGB1 — ITGA9 (in vivo)
  • IRef Ophid Interaction: ITGB1 — ITGA9 (aggregation, confirmational text mining)
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/VEGFD (dimer) complex (ITGA9-ITGB1-FIGF) → VEGFD (dimer) complex (FIGF) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/VEGFD (dimer) complex (ITGA9-ITGB1-FIGF) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: VEGFD (dimer) complex (FIGF) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin/vWF complex (ITGA9-ITGB1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: VCAM1 (VCAM1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: VCAM1 (VCAM1) → alpha9/beta1 Integrin/VCAM1 complex (VCAM1-ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → alpha9/beta1 Integrin/VCAM1 complex (VCAM1-ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: VEGFA (dimer) complex (VEGFA) → alpha9/beta1 Integrin/VEGFA (dimer) complex (ITGA9-ITGB1-VEGFA) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: VEGFA (dimer) complex (VEGFA) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/VEGFA (dimer) complex (ITGA9-ITGB1-VEGFA) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/Osteopontin complex (SPP1-ITGA9-ITGB1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/Osteopontin complex (SPP1-ITGA9-ITGB1) → Osteopontin (SPP1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → Osteopontin (SPP1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin/Osteopontin complex (SPP1-ITGA9-ITGB1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin/Osteopontin complex (SPP1-ITGA9-ITGB1) → Osteopontin (SPP1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → Osteopontin (SPP1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin/TGM2 complex (TGM2-ITGA9-ITGB1) → TGM2 (TGM2) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin/TGM2 complex (TGM2-ITGA9-ITGB1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: TGM2 (TGM2) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Tenascin C complex (TNC) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Tenascin C complex (TNC) → alpha9/beta1 Integrin/Tenascin C complex (ITGA9-ITGB1-TNC) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → alpha9/beta1 Integrin/Tenascin C complex (ITGA9-ITGB1-TNC) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Factor XIII complex (F13A1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Factor XIII complex (F13A1) → alpha9/beta1 Integrin/Factor XIII complex (ITGA9-ITGB1-F13A1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → alpha9/beta1 Integrin/Factor XIII complex (ITGA9-ITGB1-F13A1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: Tenascin C complex (TNC) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: Tenascin C complex (TNC) → alpha9/beta1 Integrin/Tenascin C complex (ITGA9-ITGB1-TNC) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → alpha9/beta1 Integrin/Tenascin C complex (ITGA9-ITGB1-TNC) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Fibronectin complex (FN1) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: Fibronectin complex (FN1) → alpha9/beta1 Integrin/Fibronectin complex (ITGA9-ITGB1-FN1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Beta1 integrin cell surface interactions: alpha9/beta1 Integrin complex (ITGA9-ITGB1) → alpha9/beta1 Integrin/Fibronectin complex (ITGA9-ITGB1-FN1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/VEGFC (dimer) complex (ITGA9-ITGB1-VEGFC) → VEGFC (dimer) complex (VEGFC) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: alpha9/beta1 Integrin/VEGFC (dimer) complex (ITGA9-ITGB1-VEGFC) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Alpha9 beta1 integrin signaling events: VEGFC (dimer) complex (VEGFC) → alpha9/beta1 Integrin complex (ITGA9-ITGB1) (modification, collaborate)
    Evidence: physical interaction
In total, 219 gene pairs are associated to this article in curated databases