Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2012, PMID: 22569250

Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.

Niu, Zhaoyang; Jin, Wenxing; Zhang, Libo; Li, Xialu

RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of RBM5 is indispensable for its ability to interact with DHX15. Strikingly, we find that RBM5 stimulates the helicase activity of DHX15 in a G patch domain-dependent manner in vitro. Helicase activities play critical roles in modulating pre-mRNA splicing. Our findings thus suggest a new mechanism underlying the regulatory roles of RBM5 in pre-mRNA splicing.

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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: RBM5 — U2AF2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRPF19 — RBM5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRNP200 — RBM5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM5 — DHX15 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRPF8 — RBM5 (physical association, affinity chromatography technology)
  • IRef Intact Interaction: U2AF2 — RBM5 (direct interaction, pull down)
  • IRef Intact Interaction: PRPF19 — RBM5 (direct interaction, pull down)
  • IRef Intact Interaction: PRPF19 — RBM5 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: RBM5 — DHX15 (physical association, two hybrid)
  • IRef Intact Interaction: RBM5 — DHX15 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: RBM5 — DHX15 (direct interaction, pull down)
  • IRef Intact Interaction: Complex of U2AF2-RBM5-PRPF19-DHX15 (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PRPF8-DHX15-PRPF19-RBM5 (association, anti tag coimmunoprecipitation)
In total, 10 gene pairs are associated to this article in curated databases