Gene interactions and pathways from curated databases and text-mining
J Dent Res 2011, PMID: 21933938

Role of calcium and PKC in salivary mucous cell exocrine secretion.

Culp, D J; Zhang, Z; Evans, R L

Fluid and exocrine secretion of mucins by salivary mucous glands is regulated predominantly by parasympathetic activation of muscarinic receptors. A direct role for subsequent putative signaling steps, phospholipase C (PLC), increased intracellular calcium ([Ca(2+)](i)), and isoforms of protein kinase C (PKC) in mediating muscarinic exocrine secretion has not been elucidated, and these are potential therapeutic targets to enhance mucin secretion in hyposalivary patients. We found that muscarinic-induced mucin secretion by rat sublingual tubulo-acini was dependent upon PLC activation and the subsequent increase in [Ca(2+)](i), and further identified a transient PKC-independent component of secretion dependent upon Ca(2+) release from intracellular stores, whereas sustained secretion required entry of extracellular Ca(2+). Interactions among carbachol, PKC inhibitors, phorbol 12-myristate 13-acetate, and thapsigargin to modulate [Ca(2+)](i) implicated conventional PKC isoforms in mediating sustained secretion. With increasing times during carbachol perfusion of glands, in situ, PKC-α redistributed across glandular membrane compartments and underwent a rapid and persistent accumulation near the luminal borders of mucous cells. PKC-β1 displayed transient localization near luminal borders, whereas the novel PKCs, PKC-δ or PKC-ε, displayed little or no redistribution in mucous cells. Collective results implicate synergistic interactions between diacylglycerol (DAG) and increasing [Ca(2+)](i) levels to activate cPKCs in mediating sustained muscarinic-induced secretion.

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Text Mining Data

mucin → PLC: " We found that muscarinic induced mucin secretion by rat sublingual tubulo-acini was dependent upon PLC activation and the subsequent increase in [ Ca ( 2+ ) ] ( i ), and further identified a transient PKC independent component of secretion dependent upon Ca ( 2+ ) release from intracellular stores, whereas sustained secretion required entry of extracellular Ca ( 2+ ) "

Manually curated Databases

No curated data.