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UCSC Genome Browser Gene Interaction Graph
Gene interactions and pathways from curated databases and text-mining
J Cell Biochem 2003, PMID: 12938160

Identification of an IQGAP1/AKAP79 complex in beta-cells.

Nauert, J Brian; Rigas, Johanna D; Lester, Linda B

IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, beta-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca(++)/CaM) and the small molecular weight guanine nucleotide triphosphatases (GTPases), Cdc42, and Rac1. Here we identify a novel IQGAP1 scaffolding function by isolating the cyclic AMP dependent kinase (PKA) with IQGAP1. IQGAP1 was co-purified with PKA using 5'-cyclic AMP (cAMP) affinity chromatography and PKA activity was co-immunoprecipitated with IQGAP1 using an anti-IQGAP1 antibody. The association of IQGAP1 with PKA was shown to occur through a direct interaction between A kinase anchoring protein 79 (AKAP79) and the carboxyl-terminal domain of IQGAP1. This suggests that cAMP/PKA may be coupled with Ca(++)/CaM and GTPases through an IQGAP1/AKAP79 complex.

Document information provided by NCBI PubMed

Text Mining Data

IQGAP1 → calcium/calmodulin: " The binding of IQGAP1 to its partners is regulated by calcium/calmodulin ( Ca ( ++ ) /CaM ) and the small molecular weight guanine nucleotide triphosphatases ( GTPases ), Cdc42, and Rac1 "

Manually curated Databases

  • IRef Biogrid Interaction: IQGAP1 — PRKACA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IQGAP1 — PRKACA (association, biochemical)
  • IRef Biogrid Interaction: IQGAP1 — AKAP5 (direct interaction, pull down)
  • IRef Hprd Interaction: IQGAP1 — PRKACA (in vitro)
  • IRef Hprd Interaction: IQGAP1 — PRKACA (in vivo)
  • IRef Innatedb Interaction: IQGAP1 — AKAP5 (unknown, -)
  • Reactome Reaction: PRKAR2A → IQGAP1 (indirect_complex)
  • Reactome Reaction: PRKAR2B → PRKACB (reaction)
  • Reactome Reaction: PRKAR1A → PRKACA (reaction)
  • Reactome Reaction: PRKAR1A → PRKACG (reaction)
  • Reactome Reaction: PRKAR1A → IQGAP1 (indirect_complex)
  • Reactome Reaction: PRKAR1A → AKAP5 (indirect_complex)
  • Reactome Reaction: PRKAR1B → IQGAP1 (indirect_complex)
  • Reactome Reaction: PRKACB → AKAP5 (reaction)
  • Reactome Reaction: PRKAR1B → PRKACA (reaction)
  • Reactome Reaction: PRKAR2B → PRKACA (reaction)
  • Reactome Reaction: PRKAR2A → PRKACA (reaction)
  • Reactome Reaction: PRKAR1B → PRKACB (reaction)
  • Reactome Reaction: PRKACG → IQGAP1 (reaction)
  • Reactome Reaction: PRKAR2A → AKAP5 (indirect_complex)
  • Reactome Reaction: PRKACG → AKAP5 (reaction)
  • Reactome Reaction: PRKAR1B → PRKACG (reaction)
  • Reactome Reaction: PRKACA → IQGAP1 (reaction)
  • Reactome Reaction: PRKAR1B → AKAP5 (indirect_complex)
  • Reactome Reaction: PRKAR2B → AKAP5 (indirect_complex)
  • Reactome Reaction: PRKAR2A → PRKACB (reaction)
  • Reactome Reaction: PRKAR2B → IQGAP1 (indirect_complex)
  • Reactome Reaction: PRKAR2A → PRKACG (reaction)
  • Reactome Reaction: AKAP5 → IQGAP1 (direct_complex)
  • Reactome Reaction: PRKACB → IQGAP1 (reaction)
  • Reactome Reaction: PRKACA → AKAP5 (reaction)
  • Reactome Reaction: PRKAR2B → PRKACG (reaction)
  • Reactome Reaction: PRKAR1A → PRKACB (reaction)
In total, 27 gene pairs are associated to this article in curated databases