J Leukoc Biol 2002,
PMID: 11818442
Park, Heonyong; Park, Sang Gyu; Lee, Joong-Won; Kim, Taeho; Kim, Gyuyoup; Ko, Young-Gyu; Kim, Sunghoon
An aminoacyl-tRNA synthetase-associated factor, p43, was recently shown to be secreted to induce a proinflammatory response. Because a proinflammatory response involves the cell-cell adhesion between endothelial and immune cells, we first examined the mechanism of p43-induced cell-cell adhesion of myelomonocytic leukemia cells. Intercellular adhesion molecule-1 (ICAM-1) was up-regulated by p43 and mediated p43-induced cell-cell adhesion via the interaction with LFA-1 or Mac-1. We also investigated p43-stimulated signaling pathways involved in the homotypic THP-1 cell adhesion. Because the specific inhibitors for PI3-K (phosphatidylinositol 3-kinase), ERK (extracellular signal-regulating kinase), and p38 MAPK (mitogen-activated protein kinase) blocked p43-stimulated ICAM-1 expression and homotypic THP-1 cell adhesion, these kinases were responsible for p43-induced cell-cell adhesion. p43-Dependent activation of ERK was inhibited by PI3-K inhibitors, and the activation of p38 MAPK was not. Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK-dependent up-regulation of ICAM-1.
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Text Mining Data
Intercellular adhesion molecule-1 → p43: "
Intercellular adhesion molecule-1 ( ICAM-1 ) was
up-regulated by
p43 and mediated p43 induced cell-cell adhesion via the interaction with LFA-1 or Mac-1
"
ICAM-1 → p43: "
Because the specific inhibitors for PI3-K ( phosphatidylinositol 3-kinase ), ERK ( extracellular signal regulating kinase ), and p38 MAPK ( mitogen activated protein kinase ) blocked p43 stimulated ICAM-1 expression and homotypic THP-1 cell adhesion, these kinases were responsible for p43 induced cell-cell adhesion
"
p43-Dependent → ERK: "
p43-Dependent activation of ERK was inhibited by PI3-K inhibitors, and the activation of p38 MAPK was not
"
p43-Dependent → PI3-K: "
p43-Dependent activation of ERK was inhibited by PI3-K inhibitors, and the activation of p38 MAPK was not
"
ERK → PI3-K: "
p43-Dependent activation of ERK was inhibited by PI3-K inhibitors, and the activation of p38 MAPK was not
"
p38 → MAPK: "
Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK dependent up-regulation of ICAM-1
"
PI3-K/ERK- → MAPK: "
Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK dependent up-regulation of ICAM-1
"
ICAM-1 → MAPK: "
Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK dependent up-regulation of ICAM-1
"
PI3-K/ERK- → MAPK: "
Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK dependent up-regulation of ICAM-1
"
Manually curated Databases
No curated data.