Gene interactions and pathways from curated databases and text-mining
Hum Mol Genet 2001, PMID: 11726552

Fanconi anemia protein, FANCA, associates with BRG1, a component of the human SWI/SNF complex.

Otsuki, T; Furukawa, Y; Ikeda, K; Endo, H; Yamashita, T; Shinohara, A; Iwamatsu, A; Ozawa, K; Liu, J M

Fanconi anemia (FA) is a genetic disorder that predisposes to hematopoietic failure, birth defects and cancer. We identified an interaction between the FA protein, FANCA and brm-related gene 1 (BRG1) product. BRG1 is a subunit of the SWI/SNF complex, which remodels chromatin structure through a DNA-dependent ATPase activity. FANCA was demonstrated to associate with the endogenous SWI/SNF complex. We also found a significant increase in the molecular chaperone, glucose-regulated protein 94 (GRP94) among BRG1-associated factors isolated from a FANCA-mutant cell line, which was not seen in either a normal control cell line or the mutant line complemented by wild-type FANCA. Despite this specific difference, FANCA did not appear to be absolutely required for in vitro chromatin remodeling. Finally, we demonstrated co-localization in the nucleus between transfected FANCA and BRG1. The physiological action of FANCA on the SWI/SNF complex remains to be clarified, but our work suggests that FANCA may recruit the SWI/SNF complex to target genes, thereby enabling coupled nuclear functions such as transcription and DNA repair.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: SMARCA4 — SMARCB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCC1 — SMARCA4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSP90B1 — SMARCA4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCA4 — SMARCC2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCC1 — HSP90B1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCA4 — FANCA (direct interaction, two hybrid)
  • IRef Biogrid Interaction: SMARCA4 — FANCA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSP90B1 — SMARCC2 (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: SMARCD3 — FANCA (in vivo)
  • IRef Hprd Interaction: SMARCC1 — FANCA (in vivo)
  • IRef Hprd Interaction: SMARCA4 — FANCA (two hybrid)
  • IRef Hprd Interaction: SMARCA4 — FANCA (in vitro)
  • IRef Hprd Interaction: SMARCA4 — FANCA (in vivo)
  • IRef Intact Interaction: SMARCA4 — FANCA (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: SMARCA4 — FANCA (physical association, two hybrid)
  • IRef Intact Interaction: SMARCA4 — FANCA (colocalization, fluorescence microscopy)
  • IRef Ophid Interaction: SMARCD3 — FANCA (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SMARCC1 — FANCA (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SMARCA4 — FANCA (aggregation, confirmational text mining)
In total, 9 gene pairs are associated to this article in curated databases