Gene interactions and pathways from curated databases and text-mining
Cell 2000, PMID: 10966108

Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis.

Ikura, T; Ogryzko, V V; Grigoriev, M; Groisman, R; Wang, J; Horikoshi, M; Scully, R; Qin, J; Nakatani, Y

It is well known that histone acetylases are important chromatin modifiers and that they play a central role in chromatin transcription. Here, we present evidence for novel roles of histone acetylases. The TIP60 histone acetylase purifies as a multimeric protein complex. Besides histone acetylase activity on chromatin, the TIP60 complex possesses ATPase, DNA helicase, and structural DNA binding activities. Ectopic expression of mutated TIP60 lacking histone acetylase activity results in cells with defective double-strand DNA break repair. Importantly, the resulting cells lose their apoptotic competence, suggesting a defect in the cells' ability to signal the existence of DNA damage to the apoptotic machinery. These results indicate that the histone acetylase TIP60-containing complex plays a role in DNA repair and apoptosis.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: KAT5 — TRRAP (association, biochemical)
  • IRef Biogrid Interaction: KAT5 — TRRAP (colocalization, biochemical)
  • IRef Biogrid Interaction: KAT2B — TRRAP (association, biochemical)
  • IRef Biogrid Interaction: ACTB — KAT5 (association, biochemical)
  • IRef Biogrid Interaction: KAT5 — ACTL6A (association, biochemical)
  • IRef Biogrid Interaction: RUVBL1 — RUVBL2 (direct interaction, pull down)
  • MIPS CORUM TIP60 histone acetylase complex: TIP60 histone acetylase complex complex (ACTL6A-KAT5-RUVBL1-RUVBL2-TRRAP)
  • IRef Corum Interaction: Complex of 11 proteins (association, cosedimentation through density gradient)
  • Gene Ontology Complexes NuA4 histone acetyltransferase complex: NuA4 histone acetyltransferase complex complex (EPC2-KAT5-TRRAP-EPC1-EP400-DMAP1-ING3)
  • IRef Hprd Interaction: RUVBL1 — RUVBL2 (in vitro)
  • IRef Hprd Interaction: RUVBL1 — RUVBL2 (in vivo)
  • IRef Hprd Interaction: RUVBL1 — RUVBL2 (two hybrid)
  • IRef Intact Interaction: RUVBL2 — RUVBL2 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of RUVBL1-RUVBL2-ACTL6A-TRRAP-ACTB-KAT5 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: RUVBL1 — RUVBL2 (physical association, anti tag coimmunoprecipitation)
In total, 37 gene pairs are associated to this article in curated databases