Gene interactions and pathways from curated databases and text-mining
Zhongguo Yao Li Xue Bao 1999, PMID: 10452106

Inhibitory effects of nitric oxide and interleukin-10 on production of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6 in mouse alveolar macrophages.

Qiu, H B; Chen, D C; Pan, J Q; Liu, D W; Ma, S

OBJECTIVE

To observe the effects of nitric oxide and interleukin-10 (IL-10) on inflammatory reaction in mouse alveolar macrophages (AM).

METHODS

AM from mice were stimulated by lipopolysaccharides (LPS) 10 mg.L-1 and nitric-oxide synthase inhibitor, S-methylisothiorea sulfate (SMT) or nitric-oxide donor, S-nitroso-N-acetyl-D, L-penicillamine (SNAP). The production of tumor necrosis factor alpha (TNF alpha), IL-1 beta, IL-6, and IL-10 by AM were measured by ELISA.

RESULTS

After LPS-stimulation, TNF alpha, IL-1 beta, and IL-6 peaked at 6, 12, and 24 h, respectively by AM. SMT inhibited LPS-induced nitric oxide release and increased IL-1 beta and IL-6 secretions in AM, but the TNF alpha levels remained unchanged. SNAP had inhibitory effects on IL-1 beta and IL-6 secretions in a concentration-dependent manner, but exerted no effect on TNF alpha release. TNF alpha, IL-1 beta, and IL-6 secretions were inhibited by recombinant IL-10, but the cytokines release was upregulated by anti-IL-10 monoclonal antibody.

CONCLUSIONS

Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS-induced TNF alpha, IL-1 beta, and IL-6 secretions in mouse AM.

Document information provided by NCBI PubMed

Text Mining Data

interleukin-1 — interleukin-10: " Inhibitory effects of nitric oxide and interleukin-10 on production of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6 in mouse alveolar macrophages "

interleukin-1 beta — interleukin-10: " Inhibitory effects of nitric oxide and interleukin-10 on production of tumor necrosis factor alpha, interleukin-1 beta , and interleukin-6 in mouse alveolar macrophages "

IL-1 beta → TNF alpha: " SMT inhibited LPS induced nitric oxide release and increased IL-1 beta and IL-6 secretions in AM, but the TNF alpha levels remained unchanged "

IL-6 → TNF alpha: " SMT inhibited LPS induced nitric oxide release and increased IL-1 beta and IL-6 secretions in AM, but the TNF alpha levels remained unchanged "

TNF alpha ⊣ IL-10: " TNF alpha , IL-1 beta, and IL-6 secretions were inhibited by recombinant IL-10 , but the cytokines release was upregulated by anti-IL-10 monoclonal antibody "

IL-1 beta ⊣ IL-10: " TNF alpha, IL-1 beta , and IL-6 secretions were inhibited by recombinant IL-10 , but the cytokines release was upregulated by anti-IL-10 monoclonal antibody "

IL-6 ⊣ IL-10: " TNF alpha, IL-1 beta, and IL-6 secretions were inhibited by recombinant IL-10 , but the cytokines release was upregulated by anti-IL-10 monoclonal antibody "

TNF alpha → IL-10: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha , IL-1 beta, and IL-6 secretions in mouse AM "

TNF alpha → LPS: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha , IL-1 beta, and IL-6 secretions in mouse AM "

IL-1 beta → IL-10: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha, IL-1 beta , and IL-6 secretions in mouse AM "

IL-1 beta → LPS: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha, IL-1 beta , and IL-6 secretions in mouse AM "

IL-6 → IL-10: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha, IL-1 beta, and IL-6 secretions in mouse AM "

IL-6 → LPS: " Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS induced TNF alpha, IL-1 beta, and IL-6 secretions in mouse AM "

Manually curated Databases

No curated data.