Human Gene TTK (ENST00000369798.7_7) from GENCODE V47lift37
  Description: TTK protein kinase, transcript variant 1 (from RefSeq NM_003318.5)
Gencode Transcript: ENST00000369798.7_7
Gencode Gene: ENSG00000112742.10_13
Transcript (Including UTRs)
   Position: hg19 chr6:80,714,366-80,752,244 Size: 37,879 Total Exon Count: 22 Strand: +
Coding Region
   Position: hg19 chr6:80,715,561-80,751,919 Size: 36,359 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:80,714,366-80,752,244)mRNA (may differ from genome)Protein (857 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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MalacardsMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TTK_HUMAN
DESCRIPTION: RecName: Full=Dual specificity protein kinase TTK; EC=2.7.12.1; AltName: Full=Phosphotyrosine picked threonine-protein kinase; Short=PYT;
FUNCTION: Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Inhibited by the ATP-competitive kinase inhibitor, SP600125.
TISSUE SPECIFICITY: Present in rapidly proliferating cell lines.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAA61239.1; Type=Erroneous initiation; Sequence=CAB87580.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TTK
Diseases sorted by gene-association score: chronic polyneuropathy (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.59 RPKM in Testis
Total median expression: 35.42 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.2067-0.272 Picture PostScript Text
3' UTR -52.40325-0.161 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS
IPR011990 - TPR-like_helical

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF14531 - Kinase-like

SCOP Domains:
81901 - HCP-like
48452 - TPR-like
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2X9E - X-ray MuPIT 2ZMC - X-ray MuPIT 2ZMD - X-ray MuPIT 3CEK - X-ray MuPIT 3DBQ - X-ray MuPIT 3GFW - X-ray MuPIT 3H9F - X-ray MuPIT 3HMN - X-ray MuPIT 3HMO - X-ray MuPIT 3HMP - X-ray MuPIT 3VQU - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P33981
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004712 protein serine/threonine/tyrosine kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0007051 spindle organization
GO:0007052 mitotic spindle organization
GO:0007093 mitotic cell cycle checkpoint
GO:0007094 mitotic spindle assembly checkpoint
GO:0008284 positive regulation of cell proliferation
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0016310 phosphorylation
GO:0016321 female meiosis chromosome segregation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0033316 meiotic spindle assembly checkpoint
GO:0034501 protein localization to kinetochore
GO:0034502 protein localization to chromosome
GO:0046777 protein autophosphorylation
GO:0051304 chromosome separation
GO:1903096 protein localization to meiotic spindle midzone

Cellular Component:
GO:0000776 kinetochore
GO:0005737 cytoplasm
GO:0005819 spindle
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AK095599 - Homo sapiens cDNA FLJ38280 fis, clone FCBBF3005497, highly similar to DUAL SPECIFICITY PROTEIN KINASE TTK (EC 2.7.1.-).
BC032858 - Homo sapiens TTK protein kinase, mRNA (cDNA clone MGC:33286 IMAGE:5270012), complete cds.
AX775733 - Sequence 3 from Patent WO03048202.
LF208566 - JP 2014500723-A/16069: Polycomb-Associated Non-Coding RNAs.
BC000633 - Homo sapiens TTK protein kinase, mRNA (cDNA clone MGC:865 IMAGE:3343925), complete cds.
AK292460 - Homo sapiens cDNA FLJ77795 complete cds, highly similar to Homo sapiens TTK protein kinase, mRNA.
M86699 - Human kinase (TTK) mRNA, complete cds.
AK315696 - Homo sapiens cDNA, FLJ96794, Homo sapiens TTK protein kinase (TTK), mRNA.
DQ891960 - Synthetic construct clone IMAGE:100004590; FLH181845.01X; RZPDo839H09136D TTK protein kinase (TTK) gene, encodes complete protein.
DQ895150 - Synthetic construct Homo sapiens clone IMAGE:100009610; FLH181841.01L; RZPDo839H09135D TTK protein kinase (TTK) gene, encodes complete protein.
KJ892329 - Synthetic construct Homo sapiens clone ccsbBroadEn_01723 TTK gene, encodes complete protein.
KJ897713 - Synthetic construct Homo sapiens clone ccsbBroadEn_07107 TTK gene, encodes complete protein.
KJ905332 - Synthetic construct Homo sapiens clone ccsbBroadEn_14868 TTK gene, encodes complete protein.
MA444143 - JP 2018138019-A/16069: Polycomb-Associated Non-Coding RNAs.
LF377964 - JP 2014500723-A/185467: Polycomb-Associated Non-Coding RNAs.
CU674312 - Synthetic construct Homo sapiens gateway clone IMAGE:100020490 5' read TTK mRNA.
LF377965 - JP 2014500723-A/185468: Polycomb-Associated Non-Coding RNAs.
LF377967 - JP 2014500723-A/185470: Polycomb-Associated Non-Coding RNAs.
LF377968 - JP 2014500723-A/185471: Polycomb-Associated Non-Coding RNAs.
LF377971 - JP 2014500723-A/185474: Polycomb-Associated Non-Coding RNAs.
MA613541 - JP 2018138019-A/185467: Polycomb-Associated Non-Coding RNAs.
MA613542 - JP 2018138019-A/185468: Polycomb-Associated Non-Coding RNAs.
MA613544 - JP 2018138019-A/185470: Polycomb-Associated Non-Coding RNAs.
MA613545 - JP 2018138019-A/185471: Polycomb-Associated Non-Coding RNAs.
MA613548 - JP 2018138019-A/185474: Polycomb-Associated Non-Coding RNAs.
X70500 - H.sapiens mRNA for phosphotyrosine picked threonine kinase (PYT).
LF377981 - JP 2014500723-A/185484: Polycomb-Associated Non-Coding RNAs.
LF377982 - JP 2014500723-A/185485: Polycomb-Associated Non-Coding RNAs.
JD530097 - Sequence 511121 from Patent EP1572962.
MA613558 - JP 2018138019-A/185484: Polycomb-Associated Non-Coding RNAs.
MA613559 - JP 2018138019-A/185485: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K8U5, B2RDW2, E1P543, ENST00000369798.1, ENST00000369798.2, ENST00000369798.3, ENST00000369798.4, ENST00000369798.5, ENST00000369798.6, MPS1, MPS1L1, NM_003318, P33981, Q15272, Q5TCS0, Q9BW51, Q9NTM0, TTK_HUMAN, uc318hwb.1, uc318hwb.2
UCSC ID: ENST00000369798.7_7
RefSeq Accession: NM_003318.5
Protein: P33981 (aka TTK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.