Human Gene SPRY2 (ENST00000377104.4_7) from GENCODE V47lift37
  Description: sprouty RTK signaling antagonist 2, transcript variant 1 (from RefSeq NM_005842.4)
Gencode Transcript: ENST00000377104.4_7
Gencode Gene: ENSG00000136158.12_10
Transcript (Including UTRs)
   Position: hg19 chr13:80,910,111-80,915,261 Size: 5,151 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr13:80,910,893-80,911,840 Size: 948 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:80,910,111-80,915,261)mRNA (may differ from genome)Protein (315 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SPY2_HUMAN
DESCRIPTION: RecName: Full=Protein sprouty homolog 2; Short=Spry-2;
FUNCTION: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
SUBUNIT: Interacts with RAF1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Note=Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).
INDUCTION: By FGF signaling.
DOMAIN: The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
SIMILARITY: Belongs to the sprouty family.
SIMILARITY: Contains 1 SPR (sprouty) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: SPRY2
Diseases sorted by gene-association score: iga nephropathy 3* (904), adrenal cortical adenocarcinoma (16), thanatophoric dysplasia, type ii (13), legius syndrome (9)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D003993 Dibutyl Phthalate
  • D013749 Tetrachlorodibenzodioxin
  • C006780 bisphenol A
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C009505 4,4'-diaminodiphenylmethane
  • C027576 4-hydroxy-2-nonenal
  • D015124 8-Bromo Cyclic Adenosine Monophosphate
  • D015127 9,10-Dimethyl-1,2-benzanthracene
  • D000079 Acetaldehyde
  • D000082 Acetaminophen
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 46.43 RPKM in Nerve - Tibial
Total median expression: 803.56 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -220.30556-0.396 Picture PostScript Text
3' UTR -169.60782-0.217 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007875 - Sprouty

Pfam Domains:
PF05210 - Sprouty protein (Spry)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3BUM - X-ray MuPIT 3OB1 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O43597
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0019901 protein kinase binding
GO:0030291 protein serine/threonine kinase inhibitor activity
GO:0043539 protein serine/threonine kinase activator activity

Biological Process:
GO:0000132 establishment of mitotic spindle orientation
GO:0007275 multicellular organism development
GO:0007605 sensory perception of sound
GO:0008285 negative regulation of cell proliferation
GO:0009966 regulation of signal transduction
GO:0010628 positive regulation of gene expression
GO:0010801 negative regulation of peptidyl-threonine phosphorylation
GO:0016525 negative regulation of angiogenesis
GO:0030324 lung development
GO:0030335 positive regulation of cell migration
GO:0031345 negative regulation of cell projection organization
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0034260 negative regulation of GTPase activity
GO:0040037 negative regulation of fibroblast growth factor receptor signaling pathway
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0042127 regulation of cell proliferation
GO:0042472 inner ear morphogenesis
GO:0043066 negative regulation of apoptotic process
GO:0043407 negative regulation of MAP kinase activity
GO:0045165 cell fate commitment
GO:0045595 regulation of cell differentiation
GO:0046580 negative regulation of Ras protein signal transduction
GO:0048754 branching morphogenesis of an epithelial tube
GO:0051387 negative regulation of neurotrophin TRK receptor signaling pathway
GO:0051897 positive regulation of protein kinase B signaling
GO:0060425 lung morphogenesis
GO:0060437 lung growth
GO:0060449 bud elongation involved in lung branching
GO:0060541 respiratory system development
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071902 positive regulation of protein serine/threonine kinase activity
GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0005886 plasma membrane
GO:0015630 microtubule cytoskeleton
GO:0016020 membrane
GO:0032587 ruffle membrane
GO:0042995 cell projection


-  Descriptions from all associated GenBank mRNAs
  AL713749 - Homo sapiens mRNA; cDNA DKFZp547P1515 (from clone DKFZp547P1515).
LP882408 - Sequence 3 from Patent WO2017180581.
BC015745 - Homo sapiens sprouty homolog 2 (Drosophila), mRNA (cDNA clone MGC:23039 IMAGE:4863701), complete cds.
AF039843 - Homo sapiens Sprouty 2 (SPRY2) mRNA, complete cds.
BX648582 - Homo sapiens mRNA; cDNA DKFZp686P12172 (from clone DKFZp686P12172).
BC004205 - Homo sapiens sprouty homolog 2 (Drosophila), mRNA (cDNA clone MGC:4026 IMAGE:3533337), complete cds.
JD299965 - Sequence 280989 from Patent EP1572962.
JD567120 - Sequence 548144 from Patent EP1572962.
KJ902080 - Synthetic construct Homo sapiens clone ccsbBroadEn_11474 SPRY2 gene, encodes complete protein.
JD091340 - Sequence 72364 from Patent EP1572962.
AK313810 - Homo sapiens cDNA, FLJ94428, highly similar to Homo sapiens sprouty homolog 2 (Drosophila) (SPRY2), mRNA.
KJ892979 - Synthetic construct Homo sapiens clone ccsbBroadEn_02373 SPRY2 gene, encodes complete protein.
KR710544 - Synthetic construct Homo sapiens clone CCSBHm_00013976 SPRY2 (SPRY2) mRNA, encodes complete protein.
KR710545 - Synthetic construct Homo sapiens clone CCSBHm_00013977 SPRY2 (SPRY2) mRNA, encodes complete protein.
AB464712 - Synthetic construct DNA, clone: pF1KB9824, Homo sapiens SPRY2 gene for sprouty homolog 2, without stop codon, in Flexi system.
AM392994 - Synthetic construct Homo sapiens clone IMAGE:100001840 for hypothetical protein (SPRY2 gene).
AM393598 - Synthetic construct Homo sapiens clone IMAGE:100001848 for hypothetical protein (SPRY2 gene).
CU678614 - Synthetic construct Homo sapiens gateway clone IMAGE:100022438 5' read SPRY2 mRNA.
MF125228 - Homo sapiens bio-material MLL_11932 case PVT1/SPRY2 fusion mRNA, partial sequence.
JD158260 - Sequence 139284 from Patent EP1572962.
JD434668 - Sequence 415692 from Patent EP1572962.
JD215473 - Sequence 196497 from Patent EP1572962.
LY623319 - KR 1020180134347-A/3: DIAGNOSTIC AND THERAPEUTIC METHODS FOR CANCER.
MB416862 - JP 2019518426-A/2: DIAGNOSTIC AND THERAPEUTIC METHODS FOR CANCER.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_spryPathway - Sprouty regulation of tyrosine kinase signals

Reactome (by CSHL, EBI, and GO)

Protein O43597 (Reactome details) participates in the following event(s):

R-HSA-1295604 B-RAF dissociates from S111/S120 p-SPRY2
R-HSA-934559 SPRY2 is phosphorylated by phosphorylated MNK1
R-HSA-1295609 SRC phosphorylates SPRY2 on Y55 and Y227
R-HSA-1295599 SPRY2 translocates to the plasma membrane
R-HSA-182990 Sprouty sequesters CBL away from active EGFR
R-HSA-1295632 PPA2A dephosphorylates SPRY2
R-HSA-183089 CBL binds and ubiquitinates phosphorylated Sprouty
R-HSA-1295621 CBL dissociates from ubiquitinated p-SPRY2
R-HSA-934604 Phosphorylated SPRY2 is ubiquitinated by CBL
R-HSA-1295622 SPRY2 binds CBL
R-HSA-1295613 SPRY2 binds GRB2
R-HSA-1549564 PPTN11 dephosphorylates SPRY2
R-HSA-183051 CBL ubiquitinates Sprouty
R-HSA-182971 EGFR downregulation
R-HSA-1295596 Spry regulation of FGF signaling
R-HSA-177929 Signaling by EGFR
R-HSA-5654726 Negative regulation of FGFR1 signaling
R-HSA-5654727 Negative regulation of FGFR2 signaling
R-HSA-5654732 Negative regulation of FGFR3 signaling
R-HSA-5654733 Negative regulation of FGFR4 signaling
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654738 Signaling by FGFR2
R-HSA-5654741 Signaling by FGFR3
R-HSA-5654743 Signaling by FGFR4
R-HSA-162582 Signal Transduction
R-HSA-190236 Signaling by FGFR

-  Other Names for This Gene
  Alternate Gene Symbols: B2R9J9, ENST00000377104.1, ENST00000377104.2, ENST00000377104.3, NM_005842, O43597, Q5T6Z7, SPY2_HUMAN, uc318nkd.1, uc318nkd.2
UCSC ID: ENST00000377104.4_7
RefSeq Accession: NM_005842.4
Protein: O43597 (aka SPY2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.