Human Gene OBI1 (ENST00000282003.7_7) from GENCODE V47lift37
  Description: ORC ubiquitin ligase 1 (from RefSeq NM_024546.4)
Gencode Transcript: ENST00000282003.7_7
Gencode Gene: ENSG00000152193.8_10
Transcript (Including UTRs)
   Position: hg19 chr13:79,188,424-79,233,290 Size: 44,867 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr13:79,189,715-79,233,255 Size: 43,541 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:79,188,424-79,233,290)mRNA (may differ from genome)Protein (726 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RN219_HUMAN
DESCRIPTION: RecName: Full=RING finger protein 219;
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Contains 1 RING-type zinc finger.
SEQUENCE CAUTION: Sequence=BAB14594.1; Type=Erroneous initiation; Sequence=BC028586; Type=Frameshift; Positions=614; Sequence=CAB66586.3; Type=Frameshift; Positions=614;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.22 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 205.41 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.4035-0.269 Picture PostScript Text
3' UTR -309.501291-0.240 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD

Pfam Domains:
PF13923 - Zinc finger, C3HC4 type (RING finger)

SCOP Domains:
46579 - Prefoldin
57850 - RING/U-box
58026 - Delta-sleep-inducing peptide immunoreactive peptide
57959 - Leucine zipper domain

ModBase Predicted Comparative 3D Structure on Q5W0B1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0046872 metal ion binding


-  Descriptions from all associated GenBank mRNAs
  CR936764 - Homo sapiens mRNA; cDNA DKFZp686N15250 (from clone DKFZp686N15250).
AK023511 - Homo sapiens cDNA FLJ13449 fis, clone PLACE1003025.
AL136651 - Homo sapiens mRNA; cDNA DKFZp564N1062 (from clone DKFZp564N1062).
CR936752 - Homo sapiens mRNA; cDNA DKFZp686A01276 (from clone DKFZp686A01276).
CR936754 - Homo sapiens mRNA; cDNA DKFZp686O03173 (from clone DKFZp686O03173).
BC028586 - Homo sapiens cDNA clone IMAGE:4838051, containing frame-shift errors.
JD043940 - Sequence 24964 from Patent EP1572962.
JD428927 - Sequence 409951 from Patent EP1572962.
JD089232 - Sequence 70256 from Patent EP1572962.
JD431550 - Sequence 412574 from Patent EP1572962.
JD267441 - Sequence 248465 from Patent EP1572962.
JD090785 - Sequence 71809 from Patent EP1572962.
JD377684 - Sequence 358708 from Patent EP1572962.
JD156394 - Sequence 137418 from Patent EP1572962.
BC136764 - Homo sapiens ring finger protein 219, mRNA (cDNA clone MGC:168377 IMAGE:9020754), complete cds.
BC136765 - Homo sapiens ring finger protein 219, mRNA (cDNA clone MGC:168378 IMAGE:9020755), complete cds.
JD245550 - Sequence 226574 from Patent EP1572962.
JD297347 - Sequence 278371 from Patent EP1572962.
JD549717 - Sequence 530741 from Patent EP1572962.
JD084057 - Sequence 65081 from Patent EP1572962.
AK098640 - Homo sapiens cDNA FLJ25774 fis, clone TST06518.
JD106263 - Sequence 87287 from Patent EP1572962.
JD246062 - Sequence 227086 from Patent EP1572962.
JD058706 - Sequence 39730 from Patent EP1572962.
KJ903177 - Synthetic construct Homo sapiens clone ccsbBroadEn_12571 RNF219 gene, encodes complete protein.
JD194983 - Sequence 176007 from Patent EP1572962.
JD247212 - Sequence 228236 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2RN99, C13orf7, ENST00000282003.1, ENST00000282003.2, ENST00000282003.3, ENST00000282003.4, ENST00000282003.5, ENST00000282003.6, NM_024546, OBI1 , OBI1_HUMAN, Q5W0B1, Q8TBY2, Q9H0T2, Q9H8M0, RNF219, uc317jxw.1, uc317jxw.2
UCSC ID: ENST00000282003.7_7
RefSeq Accession: NM_024546.4
Protein: Q5W0B1 (aka RN219_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.