Human Gene PRCP (ENST00000313010.8_14) from GENCODE V47lift37
  Description: prolylcarboxypeptidase, transcript variant 1 (from RefSeq NM_005040.4)
Gencode Transcript: ENST00000313010.8_14
Gencode Gene: ENSG00000137509.12_19
Transcript (Including UTRs)
   Position: hg19 chr11:82,533,978-82,611,472 Size: 77,495 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr11:82,535,948-82,611,444 Size: 75,497 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:82,533,978-82,611,472)mRNA (may differ from genome)Protein (496 aa)
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-  Comments and Description Text from UniProtKB
  ID: PCP_HUMAN
DESCRIPTION: RecName: Full=Lysosomal Pro-X carboxypeptidase; EC=3.4.16.2; AltName: Full=Angiotensinase C; AltName: Full=Lysosomal carboxypeptidase C; AltName: Full=Proline carboxypeptidase; AltName: Full=Prolylcarboxypeptidase; Short=PRCP; Flags: Precursor;
FUNCTION: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
CATALYTIC ACTIVITY: Cleavage of a -Pro-|-Xaa bond to release a C- terminal amino acid.
SUBUNIT: Homodimer.
INTERACTION: O60883:GPR37L1; NbExp=2; IntAct=EBI-2803892, EBI-2927498;
SUBCELLULAR LOCATION: Lysosome.
TISSUE SPECIFICITY: Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
SIMILARITY: Belongs to the peptidase S28 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 36.26 RPKM in Cells - Cultured fibroblasts
Total median expression: 830.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -1.5028-0.054 Picture PostScript Text
3' UTR -485.801970-0.247 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008758 - Peptidase_S28

Pfam Domains:
PF05576 - PS-10 peptidase S37
PF05577 - Serine carboxypeptidase S28

SCOP Domains:
52343 - Ferredoxin reductase-like, C-terminal NADP-linked domain
53474 - alpha/beta-Hydrolases
55681 - Class II aaRS and biotin synthetases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3N2Z - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P42785
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
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 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004180 carboxypeptidase activity
GO:0004185 serine-type carboxypeptidase activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0002155 regulation of thyroid hormone mediated signaling pathway
GO:0002353 plasma kallikrein-kinin cascade
GO:0003085 negative regulation of systemic arterial blood pressure
GO:0006508 proteolysis
GO:0007597 blood coagulation, intrinsic pathway
GO:0042593 glucose homeostasis
GO:0043312 neutrophil degranulation
GO:0043535 regulation of blood vessel endothelial cell migration
GO:0060055 angiogenesis involved in wound healing
GO:0097009 energy homeostasis
GO:2000377 regulation of reactive oxygen species metabolic process

Cellular Component:
GO:0005764 lysosome
GO:0005886 plasma membrane
GO:0035577 azurophil granule membrane
GO:0045178 basal part of cell
GO:0070062 extracellular exosome
GO:0101003 ficolin-1-rich granule membrane


-  Descriptions from all associated GenBank mRNAs
  BC001500 - Homo sapiens prolylcarboxypeptidase (angiotensinase C), mRNA (cDNA clone MGC:2202 IMAGE:3510081), complete cds.
AK091786 - Homo sapiens cDNA FLJ34467 fis, clone HLUNG2003246, highly similar to Lysosomal Pro-X carboxypeptidase precursor (EC3.4.16.2).
AX747184 - Sequence 709 from Patent EP1308459.
L13977 - Human prolylcarboxypeptidase mRNA, complete cds.
AK302386 - Homo sapiens cDNA FLJ57232 complete cds, highly similar to Lysosomal Pro-X carboxypeptidase precursor (EC3.4.16.2).
AK312919 - Homo sapiens cDNA, FLJ93368, highly similar to Homo sapiens prolylcarboxypeptidase (angiotensinase C) (PRCP),mRNA.
AB451270 - Homo sapiens PRCP mRNA for prolylcarboxypeptidase isoform 1 preproprotein, complete cds, clone: FLJ08097AAAN.
DQ893068 - Synthetic construct clone IMAGE:100005698; FLH193262.01X; RZPDo839E0578D prolylcarboxypeptidase (angiotensinase C) (PRCP) gene, encodes complete protein.
DQ896321 - Synthetic construct Homo sapiens clone IMAGE:100010781; FLH193258.01L; RZPDo839E0568D prolylcarboxypeptidase (angiotensinase C) (PRCP) gene, encodes complete protein.
AB451397 - Homo sapiens PRCP mRNA for prolylcarboxypeptidase isoform 1 preproprotein, partial cds, clone: FLJ08097AAAF.
BX537480 - Homo sapiens mRNA; cDNA DKFZp686M162 (from clone DKFZp686M162).
AK090847 - Homo sapiens cDNA FLJ33528 fis, clone BRAMY2007110, highly similar to Lysosomal Pro-X carboxypeptidase precursor (EC 3.4.16.2).
JD020659 - Sequence 1683 from Patent EP1572962.
JD027046 - Sequence 8070 from Patent EP1572962.
JD028538 - Sequence 9562 from Patent EP1572962.
JD031197 - Sequence 12221 from Patent EP1572962.
JD121287 - Sequence 102311 from Patent EP1572962.
JD244251 - Sequence 225275 from Patent EP1572962.
JD299674 - Sequence 280698 from Patent EP1572962.
JD557015 - Sequence 538039 from Patent EP1572962.
JD019978 - Sequence 1002 from Patent EP1572962.
JD033691 - Sequence 14715 from Patent EP1572962.
JD453909 - Sequence 434933 from Patent EP1572962.
JD185601 - Sequence 166625 from Patent EP1572962.
JD128409 - Sequence 109433 from Patent EP1572962.
JD391799 - Sequence 372823 from Patent EP1572962.
JD365821 - Sequence 346845 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P42785 (Reactome details) participates in the following event(s):

R-HSA-6798739 Exocytosis of azurophil granule membrane proteins
R-HSA-6800426 Exocytosis of ficolin-rich granule membrane proteins
R-HSA-158251 prekallikrein:kininogen:C1q binding protein tetramer -> kallikrein:kininogen:C1q binding protein tetramer
R-HSA-6798695 Neutrophil degranulation
R-HSA-140837 Intrinsic Pathway of Fibrin Clot Formation
R-HSA-168249 Innate Immune System
R-HSA-140877 Formation of Fibrin Clot (Clotting Cascade)
R-HSA-168256 Immune System
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: A8MU24, B2R7B7, B3KRK5, B5BU34, ENST00000313010.1, ENST00000313010.2, ENST00000313010.3, ENST00000313010.4, ENST00000313010.5, ENST00000313010.6, ENST00000313010.7, NM_005040, P42785, PCP, PCP_HUMAN, uc317pia.1, uc317pia.2
UCSC ID: ENST00000313010.8_14
RefSeq Accession: NM_005040.4
Protein: P42785 (aka PCP_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.