Human Gene MKI67 (ENST00000368654.8_9) from GENCODE V47lift37
  Description: marker of proliferation Ki-67, transcript variant 1 (from RefSeq NM_002417.5)
Gencode Transcript: ENST00000368654.8_9
Gencode Gene: ENSG00000148773.14_12
Transcript (Including UTRs)
   Position: hg19 chr10:129,894,923-129,924,687 Size: 29,765 Total Exon Count: 15 Strand: -
Coding Region
   Position: hg19 chr10:129,897,454-129,923,931 Size: 26,478 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:129,894,923-129,924,687)mRNA (may differ from genome)Protein (3256 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KI67_HUMAN
DESCRIPTION: RecName: Full=Antigen KI-67;
FUNCTION: Thought to be required for maintaining cell proliferation.
SUBUNIT: Interacts with KIF15. Binds through the FHA domain to MKI67IP.
SUBCELLULAR LOCATION: Nucleus. Nucleus, nucleolus. Chromosome. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
SIMILARITY: Contains 1 FHA domain.
WEB RESOURCE: Name=Wikipedia; Note=Ki-67 entry; URL="http://en.wikipedia.org/wiki/Ki-67_%28Biology%29";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MKI67
Diseases sorted by gene-association score: cervical intraepithelial neoplasia (18), pancreatic neuroendocrine tumor (18), bowen's disease (18), ameloblastic carcinoma (18), brain ependymoma (17), oral leukoplakia (17), acinar cell cystadenocarcinoma (16), pancreatic cystadenocarcinoma (15), leukoplakia (14), inverted papilloma (14), squamous cell carcinoma of the oral tongue (13), larynx cancer (11), keratoacanthoma (11), cavernous hemangioma (11), keratocystic odontogenic tumor (11), benign meningioma (11), necrotizing sialometaplasia (10), spitz nevus (10), sinonasal undifferentiated carcinoma (10), pulmonary vein stenosis (10), cervical squamous cell carcinoma (10), phyllode tumor (10), salivary gland adenoid cystic carcinoma (9), bladder adenocarcinoma (9), meningioma, familial (8), soft tissue sarcoma (8), endometrial adenocarcinoma (7), polymorphous low-grade adenocarcinoma (7), epithelioid trophoblastic tumor (7), urinary bladder cancer (7), hidradenocarcinoma (7), leiomyoma (7), craniopharyngioma (7), adenosarcoma (7), myxofibrosarcoma (7), mucoepidermoid carcinoma (7), embryonal sarcoma (6), bladder cancer, somatic (6), breast cancer (6), endometrial mucinous adenocarcinoma (6), ependymoma (6), benign breast phyllodes tumor (6), squamous cell carcinoma (4), breast fibroadenoma (4), inflammatory myofibroblastic tumor (4), juvenile glaucoma (4), gastrointestinal stromal tumor (3), endometrial cancer (2), prostate cancer (2), colorectal cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.64 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 68.88 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -199.20414-0.481 Picture PostScript Text
3' UTR -817.502531-0.323 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000253 - FHA_dom
IPR012568 - K167R
IPR008984 - SMAD_FHA_domain

Pfam Domains:
PF00498 - FHA domain
PF08065 - KI67R (NUC007) repeat
PF15276 - Protein phosphatase 1 binding

SCOP Domains:
49879 - SMAD/FHA domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1R21 - NMR MuPIT 2AFF - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P46013
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008022 protein C-terminus binding

Biological Process:
GO:0007049 cell cycle
GO:0007088 regulation of mitotic nuclear division
GO:0008283 cell proliferation
GO:0051983 regulation of chromosome segregation
GO:1902275 regulation of chromatin organization

Cellular Component:
GO:0005634 nucleus
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0016020 membrane
GO:0016604 nuclear body
GO:0000793 condensed chromosome


-  Descriptions from all associated GenBank mRNAs
  X65550 - H.sapiens mki67a mRNA (long type) for antigen of monoclonal antibody Ki-67.
X65551 - H.sapiens mki67a mRNA (short type) for antigen of monoclonal antibody Ki-67.
JD281003 - Sequence 262027 from Patent EP1572962.
JD315187 - Sequence 296211 from Patent EP1572962.
JD236010 - Sequence 217034 from Patent EP1572962.
JD489704 - Sequence 470728 from Patent EP1572962.
JD547397 - Sequence 528421 from Patent EP1572962.
JD194997 - Sequence 176021 from Patent EP1572962.
JD354363 - Sequence 335387 from Patent EP1572962.
JD365656 - Sequence 346680 from Patent EP1572962.
JD131468 - Sequence 112492 from Patent EP1572962.
JD463464 - Sequence 444488 from Patent EP1572962.
JD152881 - Sequence 133905 from Patent EP1572962.
JD545861 - Sequence 526885 from Patent EP1572962.
JD433542 - Sequence 414566 from Patent EP1572962.
JD545390 - Sequence 526414 from Patent EP1572962.
JD265685 - Sequence 246709 from Patent EP1572962.
JD130794 - Sequence 111818 from Patent EP1572962.
JD545470 - Sequence 526494 from Patent EP1572962.
JD272249 - Sequence 253273 from Patent EP1572962.
JD037616 - Sequence 18640 from Patent EP1572962.
JD549624 - Sequence 530648 from Patent EP1572962.
JD318420 - Sequence 299444 from Patent EP1572962.
JD445828 - Sequence 426852 from Patent EP1572962.
JD045631 - Sequence 26655 from Patent EP1572962.
JD214740 - Sequence 195764 from Patent EP1572962.
JD424491 - Sequence 405515 from Patent EP1572962.
JD199040 - Sequence 180064 from Patent EP1572962.
JD502970 - Sequence 483994 from Patent EP1572962.
JD078903 - Sequence 59927 from Patent EP1572962.
JD384584 - Sequence 365608 from Patent EP1572962.
JD358057 - Sequence 339081 from Patent EP1572962.
JD393653 - Sequence 374677 from Patent EP1572962.
JD165841 - Sequence 146865 from Patent EP1572962.
JD103135 - Sequence 84159 from Patent EP1572962.
JD526062 - Sequence 507086 from Patent EP1572962.
JD100310 - Sequence 81334 from Patent EP1572962.
JD465477 - Sequence 446501 from Patent EP1572962.
JD159822 - Sequence 140846 from Patent EP1572962.
JD110987 - Sequence 92011 from Patent EP1572962.
JD147727 - Sequence 128751 from Patent EP1572962.
JD174272 - Sequence 155296 from Patent EP1572962.
AK299254 - Homo sapiens cDNA FLJ56929 complete cds, highly similar to Antigen KI-67.
JD355305 - Sequence 336329 from Patent EP1572962.
JD094704 - Sequence 75728 from Patent EP1572962.
JD436987 - Sequence 418011 from Patent EP1572962.
JD293640 - Sequence 274664 from Patent EP1572962.
JD400479 - Sequence 381503 from Patent EP1572962.
JD205449 - Sequence 186473 from Patent EP1572962.
AJ567756 - Homo sapiens partial mRNA for KI67 Antigen (MKI67 gene), variant lacking exons 3,4 and 7.
AJ567757 - Homo sapiens partial mRNA for KI67 Antigen (MKI67 gene), variant lacking exons 3-12.
AJ567755 - Homo sapiens partial mRNA for KI67 Antigen (MKI67 gene), variant lacking part of exon 7.
JD552702 - Sequence 533726 from Patent EP1572962.
MP015152 - Sequence 355 from Patent WO2019016252.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000368654.1, ENST00000368654.2, ENST00000368654.3, ENST00000368654.4, ENST00000368654.5, ENST00000368654.6, ENST00000368654.7, KI67_HUMAN, MKI67 , NM_002417, P46013, Q5VWH2, uc318gxv.1, uc318gxv.2
UCSC ID: ENST00000368654.8_9
RefSeq Accession: NM_002417.5
Protein: P46013 (aka KI67_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.