Human Gene HCAR3 (ENST00000528880.3_6) from GENCODE V47lift37
  Description: hydroxycarboxylic acid receptor 3 (from RefSeq NM_006018.3)
Gencode Transcript: ENST00000528880.3_6
Gencode Gene: ENSG00000255398.3_10
Transcript (Including UTRs)
   Position: hg19 chr12:123,199,303-123,201,358 Size: 2,056 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr12:123,200,121-123,201,284 Size: 1,164 Coding Exon Count: 1 

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Gene AllelesRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:123,199,303-123,201,358)mRNA (may differ from genome)Protein (387 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HCAR3_HUMAN
DESCRIPTION: RecName: Full=Hydroxycarboxylic acid receptor 3; AltName: Full=G-protein coupled receptor 109B; AltName: Full=G-protein coupled receptor HM74; AltName: Full=G-protein coupled receptor HM74B; AltName: Full=Niacin receptor 2; AltName: Full=Nicotinic acid receptor 2;
FUNCTION: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expression largely restricted to adipose tissue and spleen.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/gpr109b/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: HCAR3
Diseases sorted by gene-association score: distal hereditary motor neuropathy, type ii (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -12.3074-0.166 Picture PostScript Text
3' UTR -267.70818-0.327 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR002286 - P2_purnocptor

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
53098 - Ribonuclease H-like
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P49019
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030054 cell junction


-  Descriptions from all associated GenBank mRNAs
  BC047891 - Homo sapiens niacin receptor 2, mRNA (cDNA clone MGC:57666 IMAGE:5750043), complete cds.
D10923 - Homo sapiens mRNA for HM74, complete cds.
LP895274 - Sequence 138 from Patent EP3253886.
BC038955 - Homo sapiens G protein-coupled receptor 109B, mRNA (cDNA clone IMAGE:5756984).
JD545109 - Sequence 526133 from Patent EP1572962.
JD343386 - Sequence 324410 from Patent EP1572962.
AK290930 - Homo sapiens cDNA FLJ77985 complete cds.
JD261006 - Sequence 242030 from Patent EP1572962.
JD252029 - Sequence 233053 from Patent EP1572962.
JD165168 - Sequence 146192 from Patent EP1572962.
JD234629 - Sequence 215653 from Patent EP1572962.
JD454787 - Sequence 435811 from Patent EP1572962.
JD146159 - Sequence 127183 from Patent EP1572962.
JD497032 - Sequence 478056 from Patent EP1572962.
JD202810 - Sequence 183834 from Patent EP1572962.
JD268541 - Sequence 249565 from Patent EP1572962.
JD424750 - Sequence 405774 from Patent EP1572962.
AK313212 - Homo sapiens cDNA, FLJ93713, highly similar to Homo sapiens G protein-coupled receptor 109B (GPR109B), mRNA.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P49019 (Reactome details) participates in the following event(s):

R-HSA-3296309 HCAR3 binds HCAR3 ligands
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-3296197 Hydroxycarboxylic acid-binding receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K4G5, B2R830, E9PI97, ENST00000528880.1, ENST00000528880.2, GPR109B, HCA3, HCAR3_HUMAN, HM74B, NIACR2, NM_006018, P49019, Q8NGE4, uc324fhd.1, uc324fhd.2
UCSC ID: ENST00000528880.3_6
RefSeq Accession: NM_006018.3
Protein: P49019 (aka HCAR3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.