ID:T2FA_HUMAN DESCRIPTION: RecName: Full=General transcription factor IIF subunit 1; AltName: Full=General transcription factor IIF 74 kDa subunit; AltName: Full=Transcription initiation factor IIF subunit alpha; Short=TFIIF-alpha; AltName: Full=Transcription initiation factor RAP74; FUNCTION: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. INTERACTION: P21675:TAF1; NbExp=3; IntAct=EBI-457886, EBI-491289; O94763:URI1; NbExp=3; IntAct=EBI-457886, EBI-357067; SUBCELLULAR LOCATION: Nucleus. INDUCTION: Up-regulated in response to enterovirus 71 (EV71) infection. PTM: Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases. SIMILARITY: Belongs to the TFIIF alpha subunit family. CAUTION: Was reported (PubMed:10428810) to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P35269
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000398 mRNA splicing, via spliceosome GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0006368 transcription elongation from RNA polymerase II promoter GO:0006370 7-methylguanosine mRNA capping GO:0008543 fibroblast growth factor receptor signaling pathway GO:0009615 response to virus GO:0016070 RNA metabolic process GO:0032091 negative regulation of protein binding GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter GO:0042795 snRNA transcription from RNA polymerase II promoter GO:0043085 positive regulation of catalytic activity GO:0045944 positive regulation of transcription from RNA polymerase II promoter
BC013007 - Homo sapiens general transcription factor IIF, polypeptide 1, 74kDa, mRNA (cDNA clone MGC:4144 IMAGE:3009685), complete cds. X64037 - H.sapiens mRNA for RNA polymerase II associated protein RAP74. X64002 - H.sapiens mRNA for RAP74. JD162202 - Sequence 143226 from Patent EP1572962. JD444361 - Sequence 425385 from Patent EP1572962. JD323080 - Sequence 304104 from Patent EP1572962. JD307197 - Sequence 288221 from Patent EP1572962. JD323081 - Sequence 304105 from Patent EP1572962. JD307198 - Sequence 288222 from Patent EP1572962. JD435039 - Sequence 416063 from Patent EP1572962. JD529796 - Sequence 510820 from Patent EP1572962. JD430524 - Sequence 411548 from Patent EP1572962. JD452211 - Sequence 433235 from Patent EP1572962. JD199419 - Sequence 180443 from Patent EP1572962. JD295914 - Sequence 276938 from Patent EP1572962. JD428562 - Sequence 409586 from Patent EP1572962. JD113364 - Sequence 94388 from Patent EP1572962. JD045143 - Sequence 26167 from Patent EP1572962. JD196814 - Sequence 177838 from Patent EP1572962. JD341460 - Sequence 322484 from Patent EP1572962. JD472160 - Sequence 453184 from Patent EP1572962. JD079621 - Sequence 60645 from Patent EP1572962. JD377725 - Sequence 358749 from Patent EP1572962. JD528680 - Sequence 509704 from Patent EP1572962. AK302178 - Homo sapiens cDNA FLJ50288 complete cds, highly similar to Transcription initiation factor IIF alpha subunit (EC 2.7.11.1). JD401316 - Sequence 382340 from Patent EP1572962. AK293124 - Homo sapiens cDNA FLJ54284 complete cds, highly similar to Transcription initiation factor IIF alpha subunit (EC 2.7.11.1). JD244997 - Sequence 226021 from Patent EP1572962. JD528560 - Sequence 509584 from Patent EP1572962. JD165140 - Sequence 146164 from Patent EP1572962. JD402828 - Sequence 383852 from Patent EP1572962. JD195447 - Sequence 176471 from Patent EP1572962. JD133185 - Sequence 114209 from Patent EP1572962. JD392468 - Sequence 373492 from Patent EP1572962. JD376274 - Sequence 357298 from Patent EP1572962. JD192353 - Sequence 173377 from Patent EP1572962. JD419360 - Sequence 400384 from Patent EP1572962. JD052867 - Sequence 33891 from Patent EP1572962. JD405428 - Sequence 386452 from Patent EP1572962. JD341680 - Sequence 322704 from Patent EP1572962. JD045108 - Sequence 26132 from Patent EP1572962. BC000120 - Homo sapiens general transcription factor IIF, polypeptide 1, 74kDa, mRNA (cDNA clone MGC:1732 IMAGE:3352627), complete cds. JD305278 - Sequence 286302 from Patent EP1572962. AK315240 - Homo sapiens cDNA, FLJ96242, highly similar to Homo sapiens general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1), mRNA. KJ891314 - Synthetic construct Homo sapiens clone ccsbBroadEn_00708 GTF2F1 gene, encodes complete protein. BT007097 - Homo sapiens general transcription factor IIF, polypeptide 1, 74kDa mRNA, complete cds. DQ890597 - Synthetic construct clone IMAGE:100003227; FLH164338.01X; RZPDo839F03162D general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1) gene, encodes complete protein. DQ893766 - Synthetic construct Homo sapiens clone IMAGE:100008226; FLH164334.01L; RZPDo839F03161D general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1) gene, encodes complete protein. KJ905767 - Synthetic construct Homo sapiens clone ccsbBroadEn_15437 GTF2F1 gene, encodes complete protein. AB385238 - Synthetic construct DNA, clone: pF1KB9482, Homo sapiens GTF2F1 gene for transcription initiation factor IIF subunit alpha, complete cds, without stop codon, in Flexi system. CR456798 - Homo sapiens full open reading frame cDNA clone RZPDo834A1121D for gene GTF2F1, general transcription factor IIF, polypeptide 1, 74kDa; complete cds, incl. stopcodon. CU680466 - Synthetic construct Homo sapiens gateway clone IMAGE:100017383 5' read GTF2F1 mRNA.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P35269 (Reactome details) participates in the following event(s):
R-HSA-6810234 General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene R-HSA-113429 Elongating transcript encounters a lesion in the template R-HSA-109638 Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex R-HSA-73946 Abortive initiation R-HSA-75856 Abortive Initiation Before Second Transition R-HSA-75891 Abortive Initiation After Second Transition R-HSA-167468 Abortive HIV-1 Initiation After Second Transition R-HSA-167474 Abortive HIV-1 Initiation Before Second Transition R-HSA-167477 Abortive HIV-1 initiation after formation of the first phosphodiester bond R-HSA-112395 Abortive termination of elongation after arrest R-HSA-167459 Abortive termination of HIV-1 elongation after arrest (Tat-containing elongation complex) R-HSA-167481 Abortive termination of HIV-1 elongation after arrest R-HSA-6810238 RNA polymerase II binds initiation factors at promoter of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-72103 Formation of pre-mRNPs R-HSA-75080 Formation of AT-AC A complex R-HSA-72095 Internal Methylation of mRNA R-HSA-75873 Addition of Nucleotides 5 through 9 on the growing Transcript R-HSA-76576 Addition of nucleotides 10 and 11 on the growing transcript: Third Transition R-HSA-111264 Addition of nucleotides between position +11 and +30 R-HSA-75864 Newly Formed Phosphodiester Bond Stabilized and PPi Released R-HSA-75866 Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP R-HSA-75095 Binding of TFIIE to the growing preinitiation complex R-HSA-75861 NTP Binds Active Site of RNA Polymerase II R-HSA-75862 Fall Back to Closed Pre-initiation Complex R-HSA-75949 RNA Polymerase II Promoter Opening: First Transition R-HSA-113430 Extrusion of 5'-end of 30 nt long transcript through the pore in Pol II complex R-HSA-77071 Phosphorylation (Ser5) of RNA pol II CTD R-HSA-167117 Addition of nucleotides 10 and 11 on the growing HIV-1 transcript: Third Transition R-HSA-167136 Addition of nucleotides 5 through 9 on the growing HIV-1 transcript R-HSA-167134 Newly formed phosphodiester bond stabilized and PPi released R-HSA-167098 Phosphorylation (Ser5) of RNA pol II CTD R-HSA-167111 Extrusion of 5'-end of 30 nt long HIV-1 transcript through the pore in Pol II complex R-HSA-167130 Nucleophillic attack by 3'-hydroxyl oxygen of nascent HIV-1 transcript on the Alpha phosphate of NTP R-HSA-167115 Addition of nucleotides between position +11 and +30 on HIV-1 transcript R-HSA-167118 NTP binds active site of RNA Polymerase II in HIV-1 open pre-initiation complex R-HSA-167484 Fall Back to Closed Pre-initiation Complex R-HSA-167097 HIV Promoter Opening: First Transition R-HSA-6803523 PTB and hnRNPA1 bind FGFR2 pre-mRNA to repress IIIb splicing and promote formation of FGFR2c mRNA R-HSA-6803527 ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and expression R-HSA-77068 Activation of GT R-HSA-77069 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-77073 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-77077 Capping complex formation R-HSA-156661 Formation of Exon Junction Complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-77095 Recognition and binding of the mRNA cap by the cap-binding complex R-HSA-77090 Methylation of GMP-cap by RNA Methyltransferase R-HSA-167089 Recognition and binding of the HIV-1 mRNA cap by the cap-binding complex R-HSA-167133 Activation of GT R-HSA-167128 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-167153 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-112379 Recruitment of elongation factors to form elongation complex R-HSA-112381 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-113411 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing R-HSA-113412 Pol II elongation complex moves on the template as transcript elongates R-HSA-113414 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest R-HSA-112385 Addition of nucleotides leads to transcript elongation R-HSA-112392 Resumption of elongation after recovery from pausing R-HSA-113413 TFIIS-mediated recovery of elongation from arrest R-HSA-112396 Separation of elongating transcript from template R-HSA-167150 Resumption of elongation of HIV-1 transcript after recovery from pausing R-HSA-167076 2-4 nt.backtracking of Pol II complex on the HIV-1 template leading to elongation pausing R-HSA-167077 Recruitment of elongation factors to form HIV-1 elongation complex R-HSA-167084 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-167282 2-4 nt.backtracking of Pol II complex on the HIV-1 template leading to elongation pausing R-HSA-167284 7-14 nt. Backtracking of Pol II complex on the HIV-1 template leading to elongation arrest R-HSA-167288 TFIIS-mediated recovery of HIV-1 elongation from arrest R-HSA-167292 Resumption of elongation of HIV-1 transcript after recovery from pausing R-HSA-167148 TFIIS-mediated recovery of elongation from arrest R-HSA-167090 7-14 nt. Backtracking of Pol II complex on the HIV-1 template leading to elongation arrest R-HSA-170706 Phosphorylation of NEFL by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167191 Hyperphosphorylation (Ser2) of RNA Pol II CTD by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167192 Pol II elongation complex moves on the HIV-1 template as transcript elongates R-HSA-167181 Addition of nucleotides leads to HIV-1 transcript elongation R-HSA-167197 Separation of elongating HIV-1 transcript from template R-HSA-170704 Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-167196 Recruitment of elongation factors to form HIV-1 elongation complex R-HSA-9012319 p-TEFb phosphorylates serine 2 in RNA polymerase II CTD R-HSA-113407 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-112383 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-167083 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-167072 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-9012315 ESR1:ESTG:P-TEFb recruited to paused RNA polymerase II on MYB gene R-HSA-6810235 RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of heptad repeats in the C-terminal domain R-HSA-6810233 CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-75081 Formation of AT-AC B Complex R-HSA-75082 ATAC spliceosome mediated Lariat formation,5' splice site cleavage R-HSA-75079 Formation of AT-AC C complex R-HSA-75083 ATAC spliceosome mediated 3' splice site cleavage, exon ligation R-HSA-109639 Formation of the closed pre-initiation complex R-HSA-77078 Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme R-HSA-77081 Formation of the CE:GMP intermediate complex R-HSA-77085 Dissociation of transcript with 5'-GMP from GT R-HSA-77083 Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA R-HSA-6797616 CCNK:CDK12 binds RNA Pol II at DNA repair genes R-HSA-113402 Formation of DSIF:NELF:early elongation complex R-HSA-167085 Formation of DSIF:NELF:HIV-1 early elongation complex R-HSA-6797606 CDK12 phosphorylates RNA Pol II CTD at DNA repair genes R-HSA-113409 Abortive termination of early transcription elongation by DSIF:NELF R-HSA-167087 Limited elongation of the HIV-1 transcript R-HSA-167478 Abortive termination of HIV-1 early transcription elongation by DSIF:NELF R-HSA-167147 Separation of abortive HIV-1 transcript from template R-HSA-6807505 RNA polymerase II transcribes snRNA genes R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167161 HIV Transcription Initiation R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-167243 Tat-mediated HIV elongation arrest and recovery R-HSA-167287 HIV elongation arrest and recovery R-HSA-73857 RNA Polymerase II Transcription R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-72165 mRNA Splicing - Minor Pathway R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167172 Transcription of the HIV genome R-HSA-6803529 FGFR2 alternative splicing R-HSA-8851708 Signaling by FGFR2 IIIa TM R-HSA-72086 mRNA Capping R-HSA-167158 Formation of the HIV-1 Early Elongation Complex R-HSA-72172 mRNA Splicing R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167290 Pausing and recovery of HIV elongation R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript R-HSA-9018519 Estrogen-dependent gene expression R-HSA-113418 Formation of the Early Elongation Complex R-HSA-74160 Gene expression (Transcription) R-HSA-8953854 Metabolism of RNA R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-5654738 Signaling by FGFR2 R-HSA-1839126 FGFR2 mutant receptor activation R-HSA-167169 HIV Transcription Elongation R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes R-HSA-8939211 ESR-mediated signaling R-HSA-162587 HIV Life Cycle R-HSA-190236 Signaling by FGFR R-HSA-5655253 Signaling by FGFR2 in disease R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-9006931 Signaling by Nuclear Receptors R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-162906 HIV Infection R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-1226099 Signaling by FGFR in disease R-HSA-212436 Generic Transcription Pathway R-HSA-162582 Signal Transduction R-HSA-5663205 Infectious disease R-HSA-5663202 Diseases of signal transduction R-HSA-1643685 Disease
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
