Human Gene DUS4L (ENST00000265720.8_4) from GENCODE V47lift37
  Description: dihydrouridine synthase 4 like, transcript variant 3 (from RefSeq NR_073002.2)
Gencode Transcript: ENST00000265720.8_4
Gencode Gene: ENSG00000105865.11_8
Transcript (Including UTRs)
   Position: hg19 chr7:107,204,416-107,218,968 Size: 14,553 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr7:107,207,516-107,218,005 Size: 10,490 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:107,204,416-107,218,968)mRNA (may differ from genome)Protein (317 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DUS4L_HUMAN
DESCRIPTION: RecName: Full=tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like; EC=1.3.1.-; AltName: Full=pp35; AltName: Full=tRNA-dihydrouridine synthase 4-like;
FUNCTION: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (By similarity).
COFACTOR: FAD (By similarity).
SIMILARITY: Belongs to the dus family. Dus4 subfamily.
SEQUENCE CAUTION: Sequence=AAD00100.1; Type=Frameshift; Positions=14;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.52 RPKM in Testis
Total median expression: 106.15 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -141.80349-0.406 Picture PostScript Text
3' UTR -189.10963-0.196 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013785 - Aldolase_TIM
IPR001269 - tRNA_hU_synthase
IPR018517 - tRNA_hU_synthase_CS

Pfam Domains:
PF01207 - Dihydrouridine synthase (Dus)

SCOP Domains:
51569 - Aldolase
51604 - Enolase C-terminal domain-like
51621 - Phosphoenolpyruvate/pyruvate domain
51658 - Xylose isomerase-like
51366 - Ribulose-phoshate binding barrel
51717 - Dihydropteroate synthetase-like
51391 - Thiamin phosphate synthase
110395 - CutC-like
110399 - ThiG-like
51395 - FMN-linked oxidoreductases
51412 - Inosine monophosphate dehydrogenase (IMPDH)
53383 - PLP-dependent transferases

ModBase Predicted Comparative 3D Structure on O95620
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0016491 oxidoreductase activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding

Biological Process:
GO:0002943 tRNA dihydrouridine synthesis
GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process


-  Descriptions from all associated GenBank mRNAs
  AK294095 - Homo sapiens cDNA FLJ60471 complete cds, highly similar to tRNA-dihydrouridine synthase 4-like (EC 1.-.-.-).
AK297195 - Homo sapiens cDNA FLJ59220 complete cds, highly similar to tRNA-dihydrouridine synthase 4-like (EC1.-.-.-).
BC111774 - Homo sapiens dihydrouridine synthase 4-like (S. cerevisiae), mRNA (cDNA clone MGC:133233 IMAGE:40033792), complete cds.
U62767 - Human PP35 mRNA, complete cds.
AK127802 - Homo sapiens cDNA FLJ45903 fis, clone OCBBF3026088.
AK308472 - Homo sapiens cDNA, FLJ98513.
KJ902194 - Synthetic construct Homo sapiens clone ccsbBroadEn_11588 DUS4L gene, encodes complete protein.
JD194451 - Sequence 175475 from Patent EP1572962.
JD486378 - Sequence 467402 from Patent EP1572962.
JD192885 - Sequence 173909 from Patent EP1572962.
JD144037 - Sequence 125061 from Patent EP1572962.
JD466905 - Sequence 447929 from Patent EP1572962.
JD323365 - Sequence 304389 from Patent EP1572962.
JD301372 - Sequence 282396 from Patent EP1572962.
JD051922 - Sequence 32946 from Patent EP1572962.
JD496360 - Sequence 477384 from Patent EP1572962.
JD049157 - Sequence 30181 from Patent EP1572962.
JD037736 - Sequence 18760 from Patent EP1572962.
JD305880 - Sequence 286904 from Patent EP1572962.
JD120290 - Sequence 101314 from Patent EP1572962.
JD299062 - Sequence 280086 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B4DLX0, DUS4L_HUMAN, ENST00000265720.1, ENST00000265720.2, ENST00000265720.3, ENST00000265720.4, ENST00000265720.5, ENST00000265720.6, ENST00000265720.7, NR_073002, O95620, Q2NKK1, uc317idn.1, uc317idn.2
UCSC ID: ENST00000265720.8_4
RefSeq Accession: NM_181581.3
Protein: O95620 (aka DUS4L_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.