ID:DHX58_HUMAN DESCRIPTION: RecName: Full=Probable ATP-dependent RNA helicase DHX58; EC=3.6.4.13; AltName: Full=Probable ATP-dependent helicase LGP2; AltName: Full=Protein D11Lgp2 homolog; AltName: Full=RIG-I-like receptor 3; Short=RLR-3; AltName: Full=RIG-I-like receptor LGP2; Short=RLR; FUNCTION: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. COFACTOR: Zinc. SUBUNIT: Monomer in the absence of dsRNA. Homodimer in the presence of dsRNA. Interacts with DDX58/RIG-I (via CARD domain), MAVS/IPS1 and DDX60. Found in a complex with DDX58/RIG-I and IFIH1/MDA5. Interacts (via helicase C-terminal domain) with non- structural protein V of Human parainfluenza 2 virus, Human parainfluenza 5 virus, measles virus, mumps virus, hendra virus and nipah virus. INTERACTION: Q7L2E3:DHX30; NbExp=2; IntAct=EBI-744193, EBI-1211456; Q9UPY3:DICER1; NbExp=2; IntAct=EBI-744193, EBI-395506; P19525:EIF2AK2; NbExp=2; IntAct=EBI-744193, EBI-640775; Q9UKV8:EIF2C2; NbExp=2; IntAct=EBI-744193, EBI-528269; P56537:EIF6; NbExp=2; IntAct=EBI-744193, EBI-372243; O15226:NKRF; NbExp=2; IntAct=EBI-744193, EBI-766011; Q9NUL3:STAU2; NbExp=2; IntAct=EBI-744193, EBI-722938; SUBCELLULAR LOCATION: Cytoplasm. INDUCTION: By interferon (IFN), virus infection, or intracellular dsRNA. DOMAIN: The repressor domain is capable of inhibiting dimerization and signaling of DDX58/RIG-I and also facilitates binding of dsRNA. SIMILARITY: Belongs to the helicase family. RLR subfamily. SIMILARITY: Contains 1 helicase ATP-binding domain. SIMILARITY: Contains 1 helicase C-terminal domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96C10
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0002376 immune system process GO:0009615 response to virus GO:0016032 viral process GO:0032480 negative regulation of type I interferon production GO:0032481 positive regulation of type I interferon production GO:0039534 negative regulation of MDA-5 signaling pathway GO:0039536 negative regulation of RIG-I signaling pathway GO:0045087 innate immune response GO:0045088 regulation of innate immune response GO:0045824 negative regulation of innate immune response GO:0051607 defense response to virus GO:1900245 positive regulation of MDA-5 signaling pathway GO:1900246 positive regulation of RIG-I signaling pathway
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
