ID:DYR_HUMAN DESCRIPTION: RecName: Full=Dihydrofolate reductase; EC=1.5.1.3; FUNCTION: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8- dihydrofolate + NADPH. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.7 uM for dihydrofolate; KM=4.0 uM for NADPH; PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. SUBUNIT: Homodimer. TISSUE SPECIFICITY: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. DISEASE: Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD) [MIM:613839]. DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. SIMILARITY: Belongs to the dihydrofolate reductase family. SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain. WEB RESOURCE: Name=Wikipedia; Note=Dihydrofolate reductase entry; URL="http://en.wikipedia.org/wiki/Dihydrofolate_reductase";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P00374
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0006729 tetrahydrobiopterin biosynthetic process GO:0006730 one-carbon metabolic process GO:0017148 negative regulation of translation GO:0031103 axon regeneration GO:0031427 response to methotrexate GO:0046452 dihydrofolate metabolic process GO:0046653 tetrahydrofolate metabolic process GO:0046654 tetrahydrofolate biosynthetic process GO:0046655 folic acid metabolic process GO:0051000 positive regulation of nitric-oxide synthase activity GO:0055114 oxidation-reduction process GO:2000121 regulation of removal of superoxide radicals
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
