Human Gene DCAF15 (ENST00000254337.11_4) from GENCODE V47lift37
  Description: DDB1 and CUL4 associated factor 15, transcript variant 1 (from RefSeq NM_138353.4)
Gencode Transcript: ENST00000254337.11_4
Gencode Gene: ENSG00000132017.11_11
Transcript (Including UTRs)
   Position: hg19 chr19:14,063,321-14,072,261 Size: 8,941 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr19:14,063,325-14,071,807 Size: 8,483 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:14,063,321-14,072,261)mRNA (may differ from genome)Protein (600 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DCA15_HUMAN
DESCRIPTION: RecName: Full=DDB1- and CUL4-associated factor 15;
FUNCTION: May be involved in ubiquitination and degradation through a DBB1-CUL4 E3 protein-ubiquitin ligase.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with DDB1 and CUL4A.
SEQUENCE CAUTION: Sequence=AAH13280.2; Type=Frameshift; Positions=89;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 49.42 RPKM in Testis
Total median expression: 690.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -190.80454-0.420 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF14939 - DDB1-and CUL4-substrate receptor 15, WD repeat

ModBase Predicted Comparative 3D Structure on Q66K64
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0016567 protein ubiquitination

Cellular Component:
GO:0032991 macromolecular complex
GO:0080008 Cul4-RING E3 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK093072 - Homo sapiens cDNA FLJ35753 fis, clone TESTI2004611.
AX747902 - Sequence 1427 from Patent EP1308459.
BC080575 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone MGC:99481 IMAGE:6580131), complete cds.
KJ895119 - Synthetic construct Homo sapiens clone ccsbBroadEn_04513 DCAF15 gene, encodes complete protein.
BC068994 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone IMAGE:6731444), partial cds.
BC002926 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone IMAGE:3953631), partial cds.
BC013280 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone IMAGE:3832598), partial cds.
BC018894 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone IMAGE:3953816).
BC018895 - Homo sapiens hypothetical protein BC002926, mRNA (cDNA clone IMAGE:3953733).
JD258238 - Sequence 239262 from Patent EP1572962.
CU680554 - Synthetic construct Homo sapiens gateway clone IMAGE:100018290 5' read LOC90379 mRNA.
KJ903545 - Synthetic construct Homo sapiens clone ccsbBroadEn_12939 DCAF15 gene, encodes complete protein.
JD203330 - Sequence 184354 from Patent EP1572962.
JD087919 - Sequence 68943 from Patent EP1572962.
JD445746 - Sequence 426770 from Patent EP1572962.
JD461501 - Sequence 442525 from Patent EP1572962.
JD124900 - Sequence 105924 from Patent EP1572962.
JD388643 - Sequence 369667 from Patent EP1572962.
LQ980374 - Sequence 20 from Patent WO2013050532.

-  Other Names for This Gene
  Alternate Gene Symbols: B3KS86, C19orf72, DCA15_HUMAN, DCAF15 , ENST00000254337.1, ENST00000254337.10, ENST00000254337.2, ENST00000254337.3, ENST00000254337.4, ENST00000254337.5, ENST00000254337.6, ENST00000254337.7, ENST00000254337.8, ENST00000254337.9, NM_138353, Q66K64, Q96DW0, Q9BU31, uc317foe.1, uc317foe.2
UCSC ID: ENST00000254337.11_4
RefSeq Accession: NM_138353.4
Protein: Q66K64 (aka DCA15_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.