Human Gene TKFC (ENST00000394900.8_5) from GENCODE V47lift37
  Description: triokinase and FMN cyclase, transcript variant 2 (from RefSeq NM_015533.4)
Gencode Transcript: ENST00000394900.8_5
Gencode Gene: ENSG00000149476.16_12
Transcript (Including UTRs)
   Position: hg19 chr11:61,100,700-61,116,714 Size: 16,015 Total Exon Count: 18 Strand: +
Coding Region
   Position: hg19 chr11:61,102,201-61,113,975 Size: 11,775 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:61,100,700-61,116,714)mRNA (may differ from genome)Protein (575 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DHAK_HUMAN
DESCRIPTION: RecName: Full=Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); Includes: RecName: Full=ATP-dependent dihydroxyacetone kinase; Short=DHA kinase; EC=2.7.1.28; EC=2.7.1.29; AltName: Full=Glycerone kinase; AltName: Full=Triokinase; AltName: Full=Triose kinase; Includes: RecName: Full=FAD-AMP lyase (cyclizing); EC=4.6.1.15; AltName: Full=FAD-AMP lyase (cyclic FMN forming); AltName: Full=FMN cyclase;
FUNCTION: Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
CATALYTIC ACTIVITY: ATP + glycerone = ADP + glycerone phosphate.
CATALYTIC ACTIVITY: ATP + D-glyceraldehyde = ADP + D- glyceraldehyde 3-phosphate.
CATALYTIC ACTIVITY: FAD = AMP + riboflavin cyclic-4',5'-phosphate.
COFACTOR: Magnesium (By similarity).
COFACTOR: Manganese or cobalt; for FAD-AMP lyase activity (By similarity).
ENZYME REGULATION: Each activity is inhibited by the substrate(s) of the other.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.5 uM for dihydroxyacetone; KM=11 uM for glyceraldehyde; pH dependence: Optimum pH is 6.6;
SUBUNIT: Homodimer (By similarity).
TISSUE SPECIFICITY: Detected in erythrocytes (at protein level).
SIMILARITY: Belongs to the dihydroxyacetone kinase (DAK) family.
SIMILARITY: Contains 1 DhaK domain.
SIMILARITY: Contains 1 DhaL domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 51.58 RPKM in Small Intestine - Terminal Ileum
Total median expression: 430.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -86.70211-0.411 Picture PostScript Text
3' UTR -957.702739-0.350 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004006 - Dak1
IPR004007 - Dak2
IPR012734 - DhaK_ATP

Pfam Domains:
PF02733 - Dak1 domain
PF02734 - DAK2 domain

SCOP Domains:
101473 - DhaL-like
82549 - DAK1/DegV-like

ModBase Predicted Comparative 3D Structure on Q3LXA3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details  Gene DetailsGene Details
Gene SorterGene Sorter  Gene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003824 catalytic activity
GO:0004371 glycerone kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016829 lyase activity
GO:0034012 FAD-AMP lyase (cyclizing) activity
GO:0046872 metal ion binding
GO:0050354 triokinase activity

Biological Process:
GO:0006071 glycerol metabolic process
GO:0008152 metabolic process
GO:0016310 phosphorylation
GO:0039534 negative regulation of MDA-5 signaling pathway
GO:0044262 cellular carbohydrate metabolic process
GO:0045087 innate immune response
GO:0045088 regulation of innate immune response
GO:0046835 carbohydrate phosphorylation
GO:0061624 fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate

Cellular Component:
GO:0005634 nucleus
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK223580 - Homo sapiens mRNA for DKFZP586B1621 protein variant, clone: FCC130A04.
AK023915 - Homo sapiens cDNA FLJ13853 fis, clone THYRO1000951, weakly similar to DIHYDROXYACETONE KINASE 2 (EC 2.7.1.29).
AK308553 - Homo sapiens cDNA, FLJ98594.
BC001341 - Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae), mRNA (cDNA clone MGC:5621 IMAGE:3462452), complete cds.
JD533916 - Sequence 514940 from Patent EP1572962.
JD187352 - Sequence 168376 from Patent EP1572962.
HM005364 - Homo sapiens clone HTL-T-51 testis tissue sperm-binding protein Li 84P mRNA, complete cds.
KJ904707 - Synthetic construct Homo sapiens clone ccsbBroadEn_14101 DAK gene, encodes complete protein.
KJ905448 - Synthetic construct Homo sapiens clone ccsbBroadEn_15031 DAK gene, encodes complete protein.
DQ138290 - Homo sapiens Dha kinase/FMN cyclase mRNA, complete cds.
DQ344550 - Homo sapiens Dha kinase/FMN cyclase splice variant (DAK) mRNA, complete cds, alternatively spliced.
BC120929 - Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40114844), partial cds.
AL110260 - Homo sapiens mRNA; cDNA DKFZp586B1621 (from clone DKFZp586B1621).
JD494532 - Sequence 475556 from Patent EP1572962.
JD494533 - Sequence 475557 from Patent EP1572962.
JD431478 - Sequence 412502 from Patent EP1572962.
JD292665 - Sequence 273689 from Patent EP1572962.
JD233380 - Sequence 214404 from Patent EP1572962.
JD403290 - Sequence 384314 from Patent EP1572962.
JD131764 - Sequence 112788 from Patent EP1572962.
JD222822 - Sequence 203846 from Patent EP1572962.
JD392041 - Sequence 373065 from Patent EP1572962.
JD203056 - Sequence 184080 from Patent EP1572962.
JD468082 - Sequence 449106 from Patent EP1572962.
JD103093 - Sequence 84117 from Patent EP1572962.
JD067678 - Sequence 48702 from Patent EP1572962.
JD451773 - Sequence 432797 from Patent EP1572962.
JD544813 - Sequence 525837 from Patent EP1572962.
JD297751 - Sequence 278775 from Patent EP1572962.
JD175480 - Sequence 156504 from Patent EP1572962.
JD239250 - Sequence 220274 from Patent EP1572962.
JD436626 - Sequence 417650 from Patent EP1572962.
JD484644 - Sequence 465668 from Patent EP1572962.
JD377405 - Sequence 358429 from Patent EP1572962.
JD264330 - Sequence 245354 from Patent EP1572962.
JD322456 - Sequence 303480 from Patent EP1572962.
JD126336 - Sequence 107360 from Patent EP1572962.
JD439490 - Sequence 420514 from Patent EP1572962.
JD122551 - Sequence 103575 from Patent EP1572962.
AK094516 - Homo sapiens cDNA FLJ37197 fis, clone BRALZ2005950.
JD168180 - Sequence 149204 from Patent EP1572962.
JD301425 - Sequence 282449 from Patent EP1572962.
JD211151 - Sequence 192175 from Patent EP1572962.
JD285309 - Sequence 266333 from Patent EP1572962.
JD520914 - Sequence 501938 from Patent EP1572962.
JD267024 - Sequence 248048 from Patent EP1572962.
JD178772 - Sequence 159796 from Patent EP1572962.
JD276567 - Sequence 257591 from Patent EP1572962.
JD201782 - Sequence 182806 from Patent EP1572962.
JD165475 - Sequence 146499 from Patent EP1572962.
JD294410 - Sequence 275434 from Patent EP1572962.
JD278031 - Sequence 259055 from Patent EP1572962.
JD216521 - Sequence 197545 from Patent EP1572962.
JD445176 - Sequence 426200 from Patent EP1572962.
JD110019 - Sequence 91043 from Patent EP1572962.
JD082736 - Sequence 63760 from Patent EP1572962.
JD237026 - Sequence 218050 from Patent EP1572962.
JD309844 - Sequence 290868 from Patent EP1572962.
JD333878 - Sequence 314902 from Patent EP1572962.
JD454999 - Sequence 436023 from Patent EP1572962.
JD377957 - Sequence 358981 from Patent EP1572962.
JD353992 - Sequence 335016 from Patent EP1572962.
JD240818 - Sequence 221842 from Patent EP1572962.
JD401895 - Sequence 382919 from Patent EP1572962.
JD172513 - Sequence 153537 from Patent EP1572962.
JD305038 - Sequence 286062 from Patent EP1572962.
JD555867 - Sequence 536891 from Patent EP1572962.
JD532716 - Sequence 513740 from Patent EP1572962.
JD097355 - Sequence 78379 from Patent EP1572962.
JD460449 - Sequence 441473 from Patent EP1572962.
JD103734 - Sequence 84758 from Patent EP1572962.
JD246747 - Sequence 227771 from Patent EP1572962.
JD090185 - Sequence 71209 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY66-373 - sucrose degradation

Reactome (by CSHL, EBI, and GO)

Protein Q3LXA3 (Reactome details) participates in the following event(s):

R-HSA-913725 viral dsRNA binds IFIH1:DAK
R-HSA-70349 DAK dimer phosphorylates D-glyceraldehyde to form D-glyceraldehyde 3-phosphate
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-168249 Innate Immune System
R-HSA-70350 Fructose catabolism
R-HSA-168256 Immune System
R-HSA-5652084 Fructose metabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: DAK , ENST00000394900.1, ENST00000394900.2, ENST00000394900.3, ENST00000394900.4, ENST00000394900.5, ENST00000394900.6, ENST00000394900.7, NM_015533, Q2L9C1, Q3LXA3, Q53EQ9, Q9BVA7, Q9H895, TKFC , TKFC_HUMAN, uc318wzu.1, uc318wzu.2
UCSC ID: ENST00000394900.8_5
RefSeq Accession: NM_015533.4
Protein: Q3LXA3 (aka DHAK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.