Human Gene AKR7A3 (ENST00000361640.5_7) from GENCODE V47lift37
  Description: aldo-keto reductase family 7 member A3 (from RefSeq NM_012067.3)
Gencode Transcript: ENST00000361640.5_7
Gencode Gene: ENSG00000162482.5_9
Transcript (Including UTRs)
   Position: hg19 chr1:19,609,067-19,615,264 Size: 6,198 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr1:19,609,225-19,615,203 Size: 5,979 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:19,609,067-19,615,264)mRNA (may differ from genome)Protein (331 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ARK73_HUMAN
DESCRIPTION: RecName: Full=Aflatoxin B1 aldehyde reductase member 3; EC=1.-.-.-; AltName: Full=AFB1 aldehyde reductase 2; Short=AFB1-AR 2;
FUNCTION: Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.
SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: AKR7A3
Diseases sorted by gene-association score: pancreas adenocarcinoma (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • C005399 9,10-phenanthrenequinone
  • D016604 Aflatoxin B1
  • C492600 (2,3,5,6-tetrafluoro-4-methoxymethylphenyl)methyl-2,2-dimethyl-3-(1-propenyl)cyclopropanecarboxylate
  • C012277 1,2-diamino-4-nitrobenzene
  • C049325 1,2-dithiol-3-thione
  • C576882 1-(2-trifluoromethoxyphenyl)-2-nitroethanone
  • C035314 1-nitropropane
  • C029141 2,2,5,7,8-pentamethyl-1-hydroxychroman
  • C010914 2,4-diaminotoluene
  • C016403 2,4-dinitrotoluene
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 149.54 RPKM in Stomach
Total median expression: 510.22 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.1061-0.346 Picture PostScript Text
3' UTR -28.00158-0.177 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001395 - Aldo/ket_red
IPR023210 - NADP_OxRdtase_dom

Pfam Domains:
PF00248 - Aldo/keto reductase family

SCOP Domains:
51430 - NAD(P)-linked oxidoreductase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2CLP - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O95154
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details   Gene Details
Gene SorterGene Sorter   Gene Sorter
 RGD   SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004033 aldo-keto reductase (NADP) activity
GO:0005515 protein binding
GO:0009055 electron carrier activity
GO:0016491 oxidoreductase activity
GO:0042802 identical protein binding

Biological Process:
GO:0006081 cellular aldehyde metabolic process
GO:0006805 xenobiotic metabolic process
GO:0022900 electron transport chain
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  KJ898428 - Synthetic construct Homo sapiens clone ccsbBroadEn_07822 AKR7A3 gene, encodes complete protein.
KR710653 - Synthetic construct Homo sapiens clone CCSBHm_00015213 AKR7A3 (AKR7A3) mRNA, encodes complete protein.
BC042420 - Homo sapiens aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase), mRNA (cDNA clone MGC:34818 IMAGE:5186889), complete cds.
GQ891370 - Homo sapiens clone HEL-S-158l epididymis secretory sperm binding protein mRNA, complete cds.
AJ271799 - Homo sapiens mRNA for aflatoxin B1 aldehyde reductase 2 (AFAR2 gene).
BC025709 - Homo sapiens aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase), mRNA (cDNA clone MGC:34394 IMAGE:5224626), complete cds.
AF040639 - Homo sapiens aflatoxin B1-aldehyde reductase mRNA, complete cds.
BC031562 - Homo sapiens aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase), mRNA (cDNA clone MGC:34393 IMAGE:5184829), complete cds.
JD308653 - Sequence 289677 from Patent EP1572962.
JD165059 - Sequence 146083 from Patent EP1572962.
JD289913 - Sequence 270937 from Patent EP1572962.
JD352138 - Sequence 333162 from Patent EP1572962.
EU176235 - Synthetic construct Homo sapiens clone IMAGE:100006584; FLH263911.01X; RZPDo839B05246D aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) (AKR7A3) gene, encodes complete protein.
EU176541 - Synthetic construct Homo sapiens clone IMAGE:100011358; FLH167825.01L; RZPDo839E08253D aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) (AKR7A3) gene, encodes complete protein.
GQ891371 - Homo sapiens clone HEL-S-159l epididymis secretory sperm binding protein mRNA, complete cds.
KJ898429 - Synthetic construct Homo sapiens clone ccsbBroadEn_07823 AKR7A3 gene, encodes complete protein.
EU831806 - Synthetic construct Homo sapiens clone HAIB:100066835; DKFZo008F0221 aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) protein (AKR7A3) gene, encodes complete protein.
EU831883 - Synthetic construct Homo sapiens clone HAIB:100066912; DKFZo004F0222 aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) protein (AKR7A3) gene, encodes complete protein.
CU693022 - Synthetic construct Homo sapiens gateway clone IMAGE:100016720 5' read AKR7A3 mRNA.
JD555212 - Sequence 536236 from Patent EP1572962.
JD394388 - Sequence 375412 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O95154 (Reactome details) participates in the following event(s):

R-HSA-5423637 AKR dimers reduce AFBDHO to AFBDOH
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: AFAR2, ARK73_HUMAN, ENST00000361640.1, ENST00000361640.2, ENST00000361640.3, ENST00000361640.4, NM_012067, O95154, Q86SR4, Q8IVN6, Q8N5V6, Q8TAX1, Q9NUC3, uc318cfk.1, uc318cfk.2
UCSC ID: ENST00000361640.5_7
RefSeq Accession: NM_012067.3
Protein: O95154 (aka ARK73_HUMAN or AR73_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.